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Genetic variation and phylogenetic analysis of rabbit haemorrhagic disease virus (rhdv) strains

100%

Hukowska-Szematowicz B. , Tokarz-Deptuła B. , Deptuła W.

Acta Biochimica Polonica

2012

tom 59

nr 4

459-465

Rabbit haemorrhagic disease virus (RHDV) belongs to the family Caliciviridae and is the etiological agent of the haemorrhagic disease, also known as rabbit plague. Its genome is a linear single-stranded (ss) RNA of 7437 nucleotides and the capsid is built from a single structural protein VP60. In connection with the discovery of new RHDV strains, there is a constant need to investigate the genetic variation of this virus and perform phylogenetic analyses which may show the evolutionary relationships among the RHDV strains. Studies on the divergence of RHDV have shown that it is genetically quite stable, although recent observations indicate that some new RHDV strains, significantly different from the original RHDV subtype and the new RHDVa subtype, are appearing. These latest findings suggest that a new group of RHDV strains has evolved. The present review summarizes the current knowledge on the genetic variation and the latest achievements in phylogenetic analyses of RHDV strains isolated in various countries.

Evaluation of Neisseria meningitidis carriage with the analysis of serogroups, genogroups and clonal complexes among Polish soldiers

63%

Korzeniewski K. , Konior M.

Polish Journal of Microbiology

2018

tom 67

nr 4

Neisseria meningitidis is an etiological factor of invasive meningococcal disease (IMD). This Gram-negative diplococcus is transmitted from person to person via droplets or through a direct physical contact with secretions of infected patients or asymptomatic carriers. The latter account for 5–10% of the general population. The aim of the study was to estimate the actual N. meningitidis carriage rate in the military environment with identification of serogroups, genogroups, sequence types and clonal complexes of the isolates detected among Polish soldiers. The study was conducted during winter seasons of 2015 and 2016 and involved 883 professional soldiers from the Armoured Brigade in Świętoszów, Poland. The material for testing were nasopharyngeal swabs obtained from study participants. The samples were tested using standard microbiological methods (culture, incubation, microscopy, biochemical and automated identification). N.meningitidis isolates were subjected to slide agglutination test (identification of serogroups), the bacterial DNA was extracted and allowed to determine genogroups, clonal complexes and sequence types. 76 soldiers were found to be carriers of N. meningitidis, they accounted for 8.6% of the study group. The meningococcal isolates mostly belonged to serogroup B. Sequence types ST-11439, ST-136, ST-1136 and the clonal complex 41/44CC were found to be predominant. Clonal complexes responsible for IMD were detected in 15.8% of carriers and 1.4% of the whole study participants. Carriage rates of N. meningitidis among Polish soldiers were found to be similar to those reported in the general population.

Phylogenetic analysis of selected strains of rabbit haemorrhagic disease virus on the basis of N-terminal fragment of the gene encoding structural protein VP60

63%

Pawlikowska M. , Hukowska-Szematowicz B. , Deptula W.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 2

129-133

The purpose of the study was the phylogenetic analysis of 16 European strains of the rabbit haemorrhagic disease (RHD) virus identified in 1989-2004, on the basis of N-terminal fragment of the gene encoding structural protein VP60. The obtained sequences of the strains were compared to the sequences of 30 strains of the virus received from the GenBank gene database. As a result of phylogenetic analysis of all the strains, they were divided into six genogroups, which were principally formed on the basis of the time of their identification, and the place of isolation of the RHD virus, which confirms the present hypothesis of genogroups creation among this virus. Furthermore, in our studies, a clear and visible difference was obtained between the classic strains in genogroups 1-5, and the strains referred to as antigen variants - genogroup 6.

Charakterystyka genetyczna niemieckich szczepów wirusa RHD (rabbit haemorrhagic disease)

63%

Pawlikowska M. , Niedźwiedzka P. , Deptula W.

Ekologia i Technika

2008

tom R. 16, nr 6

289-293

Celem obecnej pracy była charakterystyka genetyczna pięciu niemieckich szczepów wirusa RHD (Eisenhuttenstadt, Erfurt, Frankfurt, Rossi, Wika), w oparciu o wyniki analiz molekularnych fragmentu o długości 510 nukleotydów, kodującego N-końcową część białka strukturalnego VP60. Analiza genetyczna na podstawie wygenerowanych drzew filogenetycznego i homologii wskazuje na stosunkowo niewielką zmienność szczepów w obrębię badanej sekwencji, która wynosiła 1-8%. Zaznaczyć jednak należy, że pomiędzy grupą I tworzoną przez szczepy Rossi i Wika), a grupą drugą, w której znajdują się pozostałe analizowane szczepy niemieckie (Eisenhuttenstadt, Erfurt, Frankfurt) widać dystans genetyczny.

The aim of the study was genetic characteristic of five German strains of RHD virus (Eisenhuttenstadt, Erfurt, Frankfurt, Rossi, Wika) on the basis on molecular analysis of a 510 bp fragment, coding N-terminal part of structural VP60. The analysis was conducted to lead to determine the phylogenetic dependence of chosen RHD strains. The analysis of five German strains of RHD virus showed that the changeability of those strains is Low, ranging from l to 8%. Nevertheless there is a need to emphasize, that between the first group consisting of Rossi and Wika and the second group with all others analyzed strains from German (Eisenhuttenstadt, Erfurt, Frankfurt) the genetic distance is visible.