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Content available remote Genetic background of urinary incontinence - state-of-the-art and perspectives
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The paper presents an overview of the current studies attempting to determine the genetic background of urinary incontinence (UI) problems. The overview referred to the adaptations of biomedical literature from Medline, life science journals, and online books were searched from the earliest date possible to March 2015. Genetic variability studies (mostly with the use of single nucleotide polymorphism) in the context of UI are discussed. The authors indicate the variability of polymorphic forms of proteins, whose modified function may be related to the observed phenotypic symptoms: UI. The paper includes a discussion of the varied level of gene expression and the issue of defective process of the development of the urinary system in the context of UI disorders. The literature does not present any general, unequivocal description of the relationship between the genetic background and phenotype as UI. It is suggested that the methods of integration of data from various techniques (e.g. high throughputs) in reference to social, demographic, and other data may be the way of the approach used for the effective description of the relationship between genetic background and observed UI.
EN
Rabbit haemorrhagic disease virus (RHDV) belongs to the family Caliciviridae and is the etiological agent of the haemorrhagic disease, also known as rabbit plague. Its genome is a linear single-stranded (ss) RNA of 7437 nucleotides and the capsid is built from a single structural protein VP60. In connection with the discovery of new RHDV strains, there is a constant need to investigate the genetic variation of this virus and perform phylogenetic analyses which may show the evolutionary relationships among the RHDV strains. Studies on the divergence of RHDV have shown that it is genetically quite stable, although recent observations indicate that some new RHDV strains, significantly different from the original RHDV subtype and the new RHDVa subtype, are appearing. These latest findings suggest that a new group of RHDV strains has evolved. The present review summarizes the current knowledge on the genetic variation and the latest achievements in phylogenetic analyses of RHDV strains isolated in various countries.
EN
This research was carried out to investigate the genetic structure of the 20 F1s rapeseed hybrids established from five female moderate maturity lines and four early maturity male testers, to determine parents showing general combining ability (GCA) and detect crosses showing specific combining ability (SCA). Significant variance of parents and crosses for all the traits indicated the existence of significant genetic variation among the parents and their F1 cross combinations. Significant variance of parents vs. crosses revealed significant average heterosis for all the traits except first pod height and seeds per pod. High narrow-sense heritability estimates for number of branches and pods length, indicate the importance of additive genetic effects for these traits. Hybrid performance was generally better than parental performance for all the traits except number of branches and also the genetic variation of lines × testers for all the traits were more than lines and testers. Most of the crosses with high mean value of pods per plant were yielded from the parental lines with high mean value of this trait. The crosses including L41×Foma2, Zafar×R42 and L22B×R38 with significant positive SCA effects of seed yield had also high mean values (3400, 3311.3 and 2904.2 kg ha-1 , respectively) of this trait.
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Zwackhia viridis is a crustose lichen being found in forests throughout Poland. It belongs to rare species being labelled primeval forest lichens which are associated with large forest complexes. The eastern populations of Z. viridis were examined using RAPD technique in order to check whether geographic distance affects genetic diversity of the populations under study. The analysis of seven populations of that species showed large intraspecific diversity. The greatest genetic similarity occurred between populations from the north-eastern area of the country, about 300 km away from each other. In dendrograms, only these populations have simple matching distance greater than 50%.
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The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
EN
Breeding tactics and social structure are among the primary determinants of the level and distribution of genetic variation in a population, giving rise to genetic structure. However the effects of such behaviors are neither intuitively obvious nor predictive. Interpretations of genetic interactions are hampered by a lack of suitable underlying models and the resulting weak empirical data base means that we are presently unable to answer fundamental questions such as whether the social structure exhibited by a species has any necessary or consistent relationship with the extent of genetic population structure in the species. In this review I present a brief overview of recent theoretical models, and summarise results of the two most common empirical approaches; namely, genetic comparison of identified social groups, and studies of arbitrarily selected samples. Some recommendations are made with respect to future empirical investigations. Increased sophistication in classifying social complexity will be necessary to elucidate the effects of social structure and breeding tactics on partitioning of genetic variation, and to determine the true correlation between social level and genetic structure.
EN
Genomic DNA from 14 representative animals of 3 maternal lines of Bison bonasus (Linnaeus, 1758) was used for amplification of a 1026-bp fragment of mtDNA D-loop. Analysis of this mitochondria! control region demonstrated only four variable sites in the studied B. bonasus population. Nucleotide substitutions in the fragment studied were very unstable, suggesting that intralineage sequence variation can occur in B. bonasus. To estimate phylogenetic relationships within the Bouinae subfamily mtDNA control region was analysed. The phylogenetic analysis separated two species of Bison, and placed Bison bison most closely to Bos grunniens. The rate sequence divergence of the hypervariable region of the D-loop between B. bonasus and B. bison was calculated as 78.5% per Myr.
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