Wyniki wyszukiwania - Biblioteka Nauki

1

Genetic relationships between some of Malva species as determined with ISSR and ISJ markers

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Celka Z. , Szczecinska M. , Sawicki J.

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tom 19

2

An analysis of the genetic relationships and inbreeding in Tatra Shepherd Dogs depending on the type of breeding

100%

Sweklej E. , Horoszewicz E. , Niedziolka R.

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tom 68

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nr 4

3

Polymorphism of internal transcribed spacer 2 [ITS-2] sequences and genetic relationships between Fasciola hepatica and F.gigantica

100%

Semyenova S. K. , Morozova E. V. , Vasilyev V. A. , Gorokhov V. V. , Moskvin A. S. , Movsessyan S. O. , Ryskov A. P.

Acta Parasitologica

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2005

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tom 50

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nr 3

EN

Nucleotide sequences of internal transcribed spacer 2 (ITS-2) of nuclear DNA were obtained from 58 adult worms Fasciola hepatica and F. gigantica of naturally infected cattle and sheep in Russia, Ukraine, Belarus, Armenia, Uzbekistan, Turkmenistan and Tajikistan. No variation was observed between 43 liver flukes F. hepatica from Russia, Belarus, Ukraine, Armenia and Turkmenistan. Only one specimen from Armenia had a single unique transversion C-G (0.3% variation). F. gigantica from Turkmenistan, Tajikistan and Uzbekistan differed at four nucleotide transitions (1.1% variations). For comparative purpose, the ITS-2 sequences of two species from Europe, Africa, Asia, America, Australia and Oceania were used and evolution history of ITS-2 sequences of Fasciola species was reconstructed with statistical parsimony network (SPN) method. The relationships between F. hepatica and F. gigantica from different regions were discussed.

4

Genetic relationship between cultured populations of Pacific oyster revealed by RAPD analysis

100%

Aranishi F. , Okimoto T.

Journal of Applied Genetics

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2004

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tom 45

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nr 4

5

Genetic relationship of Salmonella isolates found in subcutaneous abscesses in leopard geckos (Eublepharis macularius)

86%

Zajac M. , Maluta A. , Wasyl D. , Skarzynska M. , Lalak A. , Samcik I. , Kwit R. , Szulowski K.

Journal of Veterinary Research

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2020

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tom 64

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nr 3

6

Preliminary result of a genetic polymorphism of β-lactoglobulin gene and the phylogenetic study of ten balkan and central european indigenous sheep breeds

86%

Kusza S. , Sziszkosz N. , Nagy K. , Masala A. , Kukovics S. , András J.

Acta Biochimica Polonica

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2015

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tom 62

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nr 1

109-112

EN

Genetic polymorphism at the β-lactoglobulin (β-LG) loci in indigenous sheep breeds (Tsigai, Racka, Pramenka) was determined. Altogether 904 sheep were genotyped for the presence of the A, B and C alleles of β-lactoglobulin by PCR-RFLP. The AB genotype was the most common and the β-lactoglobulin A was the most frequent in the Cokanski Tsigai (54%), while the B allele was the most common in the Rusty and the Zomborski Tsigai (59%, 60%). The C allele was found only in one individual from Serbian Cokanski flock. These results differ from those that refer to other native sheep breeds. In the Cokanski Tsigai, deviation from the Hardy-Weinberg equilibrium was detected. Genetic relationship based on β-lactoglobulin polymorphism was the closest between the Rusty and the Cokanski Tsigai among the studied populations and between sheep and goat among the other ruminants. Part of the promoter region (254 bp) of β-LG in studied sheep breeds were sequenced in order to identify polymorphisms, analyze haplotypes, and phylogenetic relationship among them. Sequencing analysis and alignment of the obtained sequences showed one haplotype. Analysis of more samples and longer parts of the promoter region of β-LG are needed to reconstruct a phylogenetic tree.

7

Genetic relationships within colonies suggest genetic monogamy in the Eurasian beaver (Castor fiber)

86%

Syruckova A. , Saveljev A. P. , Frosch C. , Durka W. , Savelyev A. A. , Munclinger P.

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nr 2

EN

Social monogamy, considered rare in mammals, has been described in two species of beaver, the Eurasion beaver (Castor fiber), and the North American beaver (Castor Canadensis). Social monogamy, however, does not necessarily imply genetic monogamy. For example, in group living mammals, females may engage in extra-pair copulations as a result of increased female mate choice opportunities. Recently, following genetic analysis, a wide range of genetic relationships among colony members have been documented in the North American beaver, including extra-pair paternity. Here, we used microsatellite loci to provide parentage estimates from colonies of the Eurasian beaver in the Kirov region, Russia. No evidence for the presence of any extra-pair young was detected. However, in two cases, we found a pair of unrelated males inhabiting a single colony. Our results suggest that while colonies may comprise both related and unrelated individuals, the genetic mating system appears to match that of the previously inferred social monogamy.

8

Comparative studies of sugar and protein kinases: genetic relationships and consensus sequences

86%

Zdyb A. , Leluk J.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2A

9

Insertion element IS3 PCR-based method for molecular analysis of enterotoxigenic Escherichia coli isolated from suckling piglets with diarrhoea

86%

Weiner M. , Dacko J. , Osek J.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

10

Evaluation of genetic diversity and genetic relationships among female Lithuanian accessions of kolomikta kiwi

86%

Cesoniene L. , Daubaras R. , Gelvonauskis B.

Journal of Fruit and Ornamental Plant Research

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2007

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tom 15

11

Genetic similarity of Pseudomonas syringae pv.tomato strains showing various virulence

72%

Kozik E. U. , Pulawska J. , Sobiczewski P.

Journal of Plant Protection Research

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2006

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tom 46

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nr 4

325-334

EN

Bacterial speck of tomato caused by Pseudomonas syringae pv. tomato appeared to be recently the most important disease on tomato in Poland. The genetic relationships among four Polish strains of race 0 P. syringae pv. tomato of different origin, isolated from tomato plants, were examined by RAPD and PCR-RFLP techniques. Amplification of bacterial DNA using 33 primers with RAPD technique showed, that similarity of strains expressed by the Nei-Li coefficient was very high (above 0.8). Next, the restriction analysis of amplified region ITS with the use of 5 endonucleases revealed, that profiles obtained from electrophoretic separation of DNA fragments were also very similar. On the basis of those analyses it was concluded that all strains tested constituted a closely related group. However, they showed various level of virulence as was demonstrated on the inoculated leaves of tomato plants growing in the greenhouse.

PL

Bakteryjna cętkowatość pomidora powodowana przez Pseudomonas syringae pv. tomato wyrządza straty o znaczeniu ekonomicznym na plantacjach pomidora w Polsce, zwłaszcza w uprawie polowej. Genetyczne relacje między 4 szczepami P. syringae pv. tomato wyizolowanymi z roślin pomidora w Polsce i zaklasyfikowanymi do rasy 0, badano metodami RAPD i PCR-RFLP. Amplifikacja bakteryjnego DNA metodą RAPD z wykorzystaniem 33 starterów wykazała, że podobieństwo między szczepami wyrażone współczynnikiem Nei-Li było bardzo wysokie (powyżej 0,8). Również analiza restrykcyjna amplifikowanego regionu ITS z użyciem 5 endonukleaz ujawniła, że profile po elektroforetycznym rozdziale fragmentów DNA były bardzo podobne. Na podstawie tych analiz stwierdzono, że badane szczepy tworzyły bardzo spokrewnioną grupę. Szczepy te wykazały jednak zróżnicowaną wirulencję na zainokulowanych liściach roślin pomidora rosnących w szklarni.

12

Genetic relationships among time of egg formation, clutch traits and traditional selection traits in laying hens

72%

Wolc A. , Bednarczyk M. , Lisowski M. , Szwaczkowski T.

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2010

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tom 19

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nr 3

EN

In a population of Rhode Island White hens heritability of egg formation, clutch characters and traditional selection traits as well as the genetic and phenotypic correlations between them were estimated via multitrait animal model. Over 1300 birds and about 4000 birds were recorded in two consecutive generations for oviposition time and traditional traits, respectively. The heritability estimates obtained for age at first egg (h2=0.42), egg weight (h2=0.50) and body weight (h2=0.42) were considerably higher than those for initial egg production (h2=0.22), clutch traits (h2 between 0.11 and 0.23) and oviposition time (h2 between 0.13 and 0.19). Both genetic and phenotypic correlations between clutch traits and traditional selection traits were low, except for initial egg production and maximal clutch length (rg=0.40 and rp=0.38). As expected, negative correlations were registered for number of clutches and average clutch length. It indicates an opportunity of selection aimed at improvement of egg production persistence by an increase in the average clutch size. Oviposition time was favourably correlated with traditional selection criteria.

13

Genetic relationship of conformation traits with lactose percentage and urea concentration in milk of Polish Holstein‑Friesian cows

72%

Satola A. , Ptak E. , Jagusiak W. , Otwinowska-Mindur A.

Animal Science Papers and Reports

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2017

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tom 35

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nr 3

EN

The objective of this study was to estimate genetic correlations of lactose percentage and urea concentration in milk with conformation traits related to udder and legs of Polish Holstein-Friesian cows. Data consisted of 5,813 test-day records and type scores of 791 primiparous cows. The analysis involved two descriptive traits (udder, feet and legs, scored from 50 to 100) and 11 linearly scored traits (describing udder: fore udder height, rear udder height, central ligament, udder depth, udder width, fore teat placement, teat length, rear teat placement; describing legs: rear legs - side view, foot angle, rear legs - rear view; on a scale of 1 to 9). Genetic correlations were calculated based on (co)variances estimated using the Bayesian method via Gibbs sampling and the multitrait animal model. Genetic correlations between lactose content and conformation traits ranged from -0.18 to 0.23, while those between milk urea concentration and conformation traits ranged between -0.02 and 0.43, respectively. Absolute values of average genetic correlations with daily lactose percentage exceeded 0.15 only for udder (descriptive trait) and several linearly scored traits, i.e. central ligament, udder depth, rear teat placement, and rear legs - rear view. Milk urea content was weakly or moderately genetically correlated with six type traits: udder, and five linearly scored traits: fore udder height, central ligament, udder width, teat length, and rear legs - side view. Absolute values of genetic correlations between these traits exceeded 0.15. Our results showed that type traits connected with udder were more highly genetically correlated with both lactose and milk urea contents than type traits describing legs. It meant that an increase in both lactose percentage and urea concentration in milk might be expected as an indirect response to selection for better udder, whereas selection for improvement of legs would not affect lactose percentage and milk urea content.

14

Assessment of genetic relationships among cultivated and wild pistachios (Pistacia vera L.) using molecular markers

72%

Pourian M. A. , Bakhshi D. , Aalami A. , Hokmabadi H.

Journal of Horticultural Research

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2019

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tom 27

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nr 1

15

Notes on genetic differentiation within the endemic Proasellus species group from Lake Ohrid [Balkan Peninsula]

72%

Kilikowska A. , Wysocka A. , Sell J. , Zietara M. , Sywula T.

Biological Letters

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2006

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tom 43

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nr 1

16

New European stands of Paramecium pentaurelia, Paramecium septaurelia, and Paramecium dodecaurelia, genetic and molecular studies

72%

Przybos E. , Prajer M. , Greczek-Stachura M. , Fokin S. I. , Rautian M. , Potekhin A.

Folia Biologica

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2005

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tom 53

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nr 3-4

17

RAPD marker analysis of polymorphism among Botrytis tulipae isolates obtained from plantations with different plant protection variants

72%

Piwoni A.

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nr 2

EN

A total of 15 isolates of B. tulipae collected from home grown tulips without chemical protection and two commercial tulip plantations were examined by RAPD fingerprint analysis. The first tulip plantation was protected by bulb treatment and foliage spraying with fungicides in the growing period and the second plantation - only by the application of fungicides in the growing period. In the previous study, a set of isolates obtained from a plantation with an extensive use of fungicides demonstrated a higher pathogenicity level measured by the inhibition of plant growth, the percentage of bulb and root necrosis in flower pot tests on forced tulips, and by the necrosis size in tests on leaf disks. The relationships between the groups and among isolates were determined by cluster analysis of mean character differences using UPGMA and NJ methods. Similarity index values ranged from 0.872 to 1; on average, the index value was 0.933. A mean similarity of genotypes indicated the highest genotype uniformity of isolates obtained from a plantation with the extensive use of fungicides. 3 groups of clusters, could be observed in the obtained dendrograms. The first cluster contains exclusively genotypes of isolates obtained from a plantation with an extensive use of fungicides, the second one only genotypes of isolates obtained from a plantation protected only by the application of fungicides in the growing period and the third - one genotype of previous group of isolates and four genotypes of isolates obtained from home grown tulips without chemical protection. The most distinct differentiation between the groups of isolates was observed by the amplification using primers G4, H20 and J13. The results of this study revealed genetic similarity between isolates which were obtained from chemically protected plantations and demonstrated a higher degree of pathogenicity in comparison to the isolates which were obtained from unprotected plants and showed a lower degree of pathogenicity.

PL

Celem badań było określenie przy użyciu markerów RAPD, zróżnicowania pomiędzy 15 izolatami B. tulipae należącymi do trzech grup izolatów uzyskanych: z plantacji tulipana, gdzie cebule zaprawiano chemicznie przed sadzeniem, a rośliny opryskiwano fungicydami w okresie wegetacji, z plantacji gdzie rośliny jedynie opryskiwano fungicydami w okresie wegetacji oraz z ogródka przydomowego, gdzie rośliny nie były poddane zabiegom ochrony. W poprzedzających badaniach, grupa izolatów uzyskanych z plantacji intensywnie chronionej fungicydami wykazała podwyższony stopień patogeniczności mierzony zahamowaniem wzrostu roślin, odsetkiem znekrotyzowania bulw i korzeni w testach patogeniczności w warunkach pędzenia oraz wielkością nekroz w testach na krążkach z liści. W przeprowadzonych badaniach, wartość indeksów podobieństwa genetycznego pomiędzy izolatami wahała się od 0,872 do 1, średnio wynosiła 0,933. Średnie podobieństwo genotypów w obrębie grup wskazuje na największe wyrównanie grupy genotypów izolatów uzyskanych z plantacji intensywnie chronionej chemicznie. Na uzyskanych dendrogramach wyróżniono 3 grupy skupień. Pierwsza z nich obejmuje wyłącznie genotypy izolatów uzyskanych z plantacji intensywnie chronionej chemicznie poprzez zaprawianie cebul i opryskiwanie roślin, druga - genotypy izolatów uzyskanych z plantacji jedynie opryskiwanej fungicydami w okresie wegetacji, natomiast w skład trzeciej, wchodzi jeden genotyp izolatu uzyskanego z w/w plantacji oraz cztery genotypy izolatów pochodzących z tulipanów uprawianych w ogródku przydomowym. W przeprowadzonych badaniach zaobserwowano zróżnicowanie genetyczne zarówno pomiędzy badanymi grupami izolatów jak również w obrębie grup. Średnie podobieństwo genotypów w obrębie grup wskazuje na największe wyrównanie genotypów izolatów uzyskanych z plantacji gdzie stosowano zaprawianie cebul oraz opryskiwanie fungicydami w okresie wegetacji i największe zróżnicowanie genotypów izolatów uzyskanych z plantacji opryskiwanej fungicydami w okresie wegetacji. Najbardziej wyraźne zróżnicowanie zaobserwowano pomiędzy grupami izolatów przy amplifikacji starterami G4, H20 oraz J13. Wyniki badań wskazują na genetyczne podobieństwo pomiędzy izolatami uzyskanymi z plantacji chronionych fungicydami, w odróżnieniu od grupy izolatów uzyskanych z roślin nie chronionych chemicznie i wykazujących niższy stopień patogeniczności.

18

Molecular analysis of enterotoxigenic, shigatoxigenic and enteroaggregative Escherichia coli strains isolated from suckling piglets with diarrhoea by the use of pulsed-field electrophoresis

72%

Weiner M. , Dacko J. , Osek J.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

19

Morphological and genetic relationships of Myosotis laxa ssp. baltica and ssp. caespitosa, and typification of M. laxa ssp. baltica

58%

Pihu S. , Opik M. , Kook E. , Reier U.

Acta Societatis Botanicorum Poloniae

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2009

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tom 78

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nr 1

37-49

EN

Intraspecific taxonomy of Myosotis laxa has been unclear for a long time. M. laxa ssp. baltica has been treated as a microendemic taxon of the Baltic Sea region, which has evolved in the Aland Islands and has spread northwards; the spread to southeast has been declared doubtful. Morphologically intermediate individuals between M. l. ssp. caespitosa and M. l. ssp. baltica exist; these have sometimes been classified as M. l. ssp. laxa. The aim of this paper is to clarify phylogenetic relationships between subspecies of M. laxa s.l. Here, M. laxa ssp. baltica is lectotypified. We proved that typical M. l. ssp. baltica does occur in the south-eastern Baltic region, namely in Estonia, using herbarium and freshly collected material. A group of plants were identified as typical M. l. ssp. baltica, but many specimens showed intermediate characters between M. laxa ssp. baltica and ssp. caespitosa. The two subspecies could be clearly differentiated neither by morphological characteristics nor by ITS sequences. M. laxa s. l. appeared to be monophyletic according to the ITS phylogeny. We propose that Myosotis laxa ssp. baltica is a coastal ecotype of Myosotis laxa, which has adapted to the fluctuating conditions of coastal habitats. Genetically, it has not yet evolved into a separate species and therefore it would be reasonable to assign it a rank of variety. However, further investigation including wider taxon and geographical sampling is needed to finally clarify the position of all subspecies.

20

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

58%

Sawicki J. , Szczecinska M.

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2011

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tom 80

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nr 3

EN

RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.