Wyniki wyszukiwania - Biblioteka Nauki

1

Myrmicinosporidium durum in Poland: a new location for this fungal ant endoparasite and updated world distribution

100%

Peral G. T. , Rutkowski T. , Wojtaszyn G. , Espadaler X.

Acta Parasitologica

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2017

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tom 62

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nr 4

2

Preparation and optimization of medicated cold cream using Caralluma adscendens var. attenuata for the treatment of Candida skin infection

100%

Sundar M. , Suresh S. , Lingakumar K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2022

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tom 103

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nr 3

3

Glucosamine-6-phosphate synthase, a novel target for antifungal agents. Molecular modelling studies in drug design

100%

Wojciechowski M. , Milewski S. , Mazerski J. , Borowski E.

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nr 3

EN

Fungal infections are a growing problem in contemporary medicine, yet only a few antifungal agents are used in clinical practice. In our laboratory we proposed the enzyme L-glutamine: D-fructose-6-phosphate amidotransferase (EC 2.6.1.16) as a new target for antifungals. The structure of this enzyme consists of two domains, N-terminal and C-terminal ones, catalysing glutamine hydrolysis and sugar-phosphate isomerisation, respectively. In our laboratory a series of potent selective inhibitors of GlcN-6-P synthase have been designed and synthesised. One group of these compounds, including the most studied N3-(4-methoxyfumaroyl)-l-2,3-diaminopropanoic acid (FMDP), behave like glutamine analogs acting as active-site-directed inactivators, blocking the N-terminal, glutamine-binding domain of the enzyme. The second group of GlcN-6-P synthase inhibitors mimic the transition state of the reaction taking place in the C-terminal sugar isomerising domain. Surprisingly, in spite of the fact that glutamine is the source of nitrogen for a number of enzymes it turned out that the glutamine analogue FMDP and its derivatives are selective against GlcN-6-P synthase and they do not block other enzymes, even belonging to the same family of glutamine amidotransferases. Our molecular modelling studies of this phenomenon revealed that even within the family of related enzymes substantial differences may exist in the geometry of the active site. In the case of the glutamine amidotransferase family the glutamine binding site of GlcN-6-P synthase fits a different region of the glutamine conformational space than other amidotransferases. Detailed analysis of the interaction pattern for the best known, so far, inhibitor of the sugar isomerising domain, namely 2-amino-2-deoxy-d-glucitol-6-phosphate (ADGP), allowed us to suggest changes in the structure of the inhibitor that should improve the interaction pattern. The novel ligand was designed and synthesised. Biological experiments confirmed our predictions. The new compound named ADMP is a much better inhibitor of glucosamine-6-phosphate synthase than ADGP.

4

The search for a genetic defect in Polish patients with chronic granulomatous disease

100%

Jurkowska M. , Kurenko-Deptuch M. , Bal J. , Roos D.

Archivum Immunologiae et Therapiae Experimentalis

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2004

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tom 52

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nr 6

5

Dermatophyte infections in persons returning from The Tropics

100%

Jeske J. , Kamerys J. , Lupa S. , Seneczko F. , Mamos K.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

6

The influence of Fusarium species on the ultrastructure of cells of potato tuber parenchyma. II. Cell organelles

100%

Mazurkiewicz-Zapalowicz K.

Hodowla Roślin, Aklimatyzacja i Nasiennictwo

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1993

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tom 37

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nr 1-2

7

Organization of antibiotic amphotericin B in model lipid membranes. A mini review

100%

Gruszecki W. I. , Gagos M. , Herec M. , Kernen P.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 1

EN

Amphotericin B (AmB) is a polyene antibiotic frequently applied in the treatment of fungal infections. According to the general understanding, the mode of action of AmB is directly related to the molecular organization of the drug in the lipid environment, in particular to the formation of pore-like molecular aggregates. Electronic absorption and fluorescence techniques were applied to investigate formation of molecular aggregates of AmB in the lipid environment of liposomes and monomolecular layers formed at the argon-water interface. It appears that AmB dimers, stabilized by van der Waals interactions, are present in the membrane environment along with the aggregates formed by a greater number of molecules. Linear dichroism measurements reveal that AmB is distributed between two fractions of molecules, differently oriented with respect to the bilayer. Molecules in one fraction remain parallel to the plane of the membrane and molecules in the other one are perpendicular. Scanning Force Microscopy imaging of the surface topography of the monolayers formed with AmB in the presence of lipids reveals formation of pore-like structures characterized by the external diameter close to 17 Å and the internal diameter close to 6 Å. All the findings are discussed in terms of importance of the molecular organization of AmB in the pharmacological action, as well as of the toxic side effects of the drug.

8

The aggregation of amphotericin B in lipid membranes - the Monte Carlo simulation

100%

Okulski W. , Gawda H.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

9

Scanning electron microscopy of Fusarium culmorum-infected kernels of ancient wheat species

84%

Packa D. , Kulik T. , Hoscik M.

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tom 63

EN

Kernels of ancient hulled wheat species, Triticum monococcum, T. dicoccon, T. spelta, and threshable wheat species, T. polonicum, T. turanicum ('Kamut®'), were studied. Fusarium culmorum-infected kernels, classified as FDKs (Fusarium damaged kernels), were smaller, shrunken, wrinkled and lighter in colour than healthy kernels. The seed coats of the former were usually damaged, and white or pinkish-white mycelium formed on their surface. FDKs showed different fungal infection levels. Characteristic structural changes were noted in endosperm cells, including loosely arranged starch granules, a partial or even complete absence of the protein matrix enveloping starch granules, the disappearance of small starch granules and the presence of damaged large starch granules. The lowest degree of damage was observed in emmer and spelt kernels.

PL

Badano ziarniaki dawnych pszenic oplewionych: Triticum monococcum, T. dicoccon, T. spelta i wymłacalnych: T. polonicum, T. turanicum ('Kamut®')- Ziarniaki porażone przez Fusarium culmorum kwalifikowane jako FDK (Fusarium damaged kernels) były mniejsze, drobniejsze, pomarszczone, jaśniejszej barwy niż ziarniaki zdrowe, zwykle z uszkodzoną okrywą owocowo-nasienną i białą lub biało-różową grzybnią na powierzchni. Poszczególne ziarniaki w obrębie frakcji FDK wykazywały zróżnicowany stopień inwazji grzybowej. W komórkach bielma obserwowano charakterystyczne zmiany strukturalne, jak: luźny układ ziaren skrobiowych, częściowy lub całkowity brak białkowej matrycy spajającej ziarna skrobi, zanik małych ziaren skrobi oraz uszkodzenia dużych ziaren skrobi. Najmniejsze uszkodzenia komórek bielma obserwowano w ziarniakach pszenicy płaskurki i orkiszu.

10

Mechanisms of Galleria mellonella cellular immune response after infection with entomopathogenic fungus Conidiobolus coronatus

84%

Ligeza-Zuber M.

Annals of Parasitology

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2012

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tom 58

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nr 4

11

Fifty Clinical Days of Medical Parasitology

84%

Wojcik A. , Blaszkowska J.

Wiadomości Parazytologiczne

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2011

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tom 57

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nr 4

12

Covid-19 and mucormycosis (Black Fungus): An epidemic within the pandemic

84%

Gambhir R. S. , Aggarwal A. , Bhardwaj A. , Kaur A. , Sohi R. K. , Mehta S.

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tom 72

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nr 3

13

Factors leading to dermatophytosis

84%

Herizchi Qadim H. , Golforoushan F. , Azimi H. , Goldust M.

Annals of Parasitology

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2013

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tom 59

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nr 2

EN

Tinea or dermatophytoses are of skin superficial and fungous infections affecting keratinized tissues such as hair, nail, and superficial layer of epidermis. This study aimed at evaluating some predisposing factors for tinea corporis, because elimination or treatment of them not only ceases spreading of the lesion but also prevents reinfection. In this descriptive cross- sectional study patients who were visited in Sina Hospital in Tabriz and had confirmed tinea corporis with direct ungal smear were selected. Other regarding were age, sex, occupation and predisposing factors. Of 76 confirmed cases, 46(60.5%) were males and 30(30.5%) were females. Tinea corporis was common in the third decade. The main predisposing factor was dry skin. Diabetes was found only in 4(5.2%) patients. According to the results of the present research, xerosis was the most common factor leading to tinea corporis in these patients rather than diabetes or lymphoma that it’s diagnosis, treatment and some simple educations may inhence improvement of tinea corporis and prevents other superficial infections too.

14

The fungi present in the sand and soil of recreational areas as possible factors of fungal infection among children: hydrolytic enzymes - a determinant of pathogenicity

84%

Kurnatowski P. , Wojcik A. , Blaszkowska J. , Goralska K.

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tom 62

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nr Suppl.

15

The response of horse erythrocytes to polyene antibiotics

84%

Knopik-Skrocka A. , Buldanczyk A.

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nr 1

16

Fungal infection of stem base of winter wheat cultivated after oats and its mixtures

84%

Lemanczyk G. , Jeske M.

Phytopathologia Polonica

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2001

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tom 22

17

Parasitism of eggs and larvae in cysts as a factor reducing population of sugar beet nematode [Heterodera schachtii] in Wielkopolska Region

84%

Dobosz R.

Phytopathologia Polonica

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2003

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tom 27

18

Targeting the fungal plasma membrane proton pump

84%

Monk B. C. , Mason A. B. , Kardos T. B. , Perlin D. S.

Acta Biochimica Polonica

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1995

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tom 42

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nr 4

EN

The need for new mechanistic classes of broad spectrum antifungal agents has prompted development of the membrane sector and ectodomain of the plasma membrane proton pumping ATPase as an antifungal target. The fungal proton pump is a highly abundant, essential enzyme in Saccharomyces cerevisiae. It belongs to the family of P-type ATPases, a class of enzymes that includes the Na+,K+-ATPase and the gastric H+,K+-ATPase. These enzymes are cell surface therapeutic targets for the cardiac glycosides and several anti-ulcer drugs, respectively. The effects of acid- -activated omeprazole show that extensive inhibition of the S. cerevisiae ATPase is fungicidal. Fungal proton pumps possess elements within their transmembrane loops that distinguish them from other P-type ATPases. These loops, such as the conformationally sensitive transmembrane loop 1+2, can attenuate the activity of the enzyme. Expression in S. cerevisiae of fully functional chimeric ATPases that contain a foreign target comprising transmembrane loops 1+2 and/or 3+4 from the fungal pathogen Candida albicans suggests that these loops operate as a domain. The chimera containing C. albicans transmembrane loops 1+2 and 3+4 provides a prototype for mutational analysis of the target region and the screening of inhibitors directed against opportunistic fungal pathogens. Panels of mutants with modified ATPase regulation or with altered cell surface cysteine residues are also described. Information about the ATPase membrane sector and ectodomain has been integrated into a model of this region.

19

Assembling the SARS-CoV genome - new method based on graph theoretical approach

84%

Blazewicz J. , Figlerowicz M. , Formanowicz P. , Kasprzak M. , Nowierski B. , Styszynski R. , Szajkowski L. , Widera P. , Wiktorczyk M.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

EN

Nowadays, scientists may learn a lot about the organisms studied just by analyzing their genetic material. This requires the development of methods of reading genomes with high accuracy. It has become clear that the knowledge of the changes occuring within a viral genome is indispensable for effective fighting of the pathogen. A good example is SARS-CoV, which was a cause of death of many people and frightened the entire world with its fast and hard to prevent propagation. Rapid development of sequencing methods, like shotgun sequencing or sequencing by hybridization (SBH), gives scientists a good tool for reading genomes. However, since sequencing methods can read fragments of up to 1000 bp only, methods for sequence assembling are required in order to read whole genomes. In this paper a new assembling method, based on graph theoretical approach, is presented. The method was tested on SARS-CoV and the results were compared to the outcome of other widely known methods.

20

Fungal glycoproteins and their biosynthetic pathway as potential targets for antifungal agents

84%

Tanner W. , Gentzsch M. , Immervoll T. , Scheinost A. , Strahl-Bolsinger S.

Acta Biochimica Polonica

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1995

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tom 42

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nr 4

EN

The yeast cell wall as a good antifungal target is discussed in general. More specifically the reaction, catalyzed by Dol-P-Man: protein O-D-mannosyltransferase is proposed as a new potential target. Six genes responsible for this endoplasmic reticulum-localized reaction have been cloned and characterized so far. Triple disruptions of these genes are either lethal or the corresponding cells have to be osmotically stabilized to survive. No inhibitors of this reaction are as yet known.