Wyniki wyszukiwania - Biblioteka Nauki

1

Gene mapping as a method for verifying sequences localization based on interspecific chromosome painting (ZOO-FISH)

100%

Bugno-Poniewierska M. , Slota B. , Pawlina K. , Potocki L. , Gurgul A. , Slota E. , Klukowska-Rotzler J.

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2014

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tom 62

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nr 1

2

Prognoza kosztów zastosowania metody FISH w badaniach składu mikrobiologicznego osadu czynnego

86%

Domańska M. , Łomotowski J.

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tom nr 12

81--83, 99

PL

Wraz ze wzrostem wymagań stawianych ściekom oczyszczonym zachodzi potrzeba wdrożenia do praktyki nowych szybkich metod pozwalających rozpoznać skład morfologiczny i ilościowy osadu czynnego, podstawowego procesu stosowanego do usuwania związków biogennych. Badania wykorzystujące metody molekularne należą do jednych z najszybciej rozwijających się metod identyfikacji mikroorganizmów. W Polsce panuje pogląd, że są to metody kosztowne o złożonych metodykach analitycznych. W artykule przedstawiono charakterystykę metody FISH (fluorescence in situ hibridization), o dużych walorach poznawczych mikrobiologicznego składu osadu wraz z prognozą kosztów badań. Wykazano zasadność wdrożenia metody FISH do monitoringu laboratoryjnego zmian składu biocenozy osadu czynnego w czasie eksploatacji oczyszczalni ścieków.

EN

Due to the increasing requirements imposed on the wastewater the need arises to put into practice new rapid methods of identifying morphological and quantitative composition of the activated sludge. The research using molecular methods is one of the rapidly growing methods for identification of microbial composition. In Poland, these methods are believed to be costly and have complex analytical methodology. The article presents the characteristics of the highly cognitive FISH (fluorescence in situ hybridization) method, along with the cost estimation of using this method. It has been demonstrated that it is justified to implement the FISH method for laboratory monitoring in the composition changes of activated sludge during operation of wastewater treatment plants.

3

Application of FISH method for preimplantation genetic diagnostics of reciprocal and Robertsonian translocations

86%

Liss J. , Kiewisz J. , Zabielska J. , Kulwikowska P. , Lukaszuk K.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 2

4

FISH mapping in cattle (Bos taurus L.) is not yet out of fashion

86%

De Lorenzi L. , Molteni L. , Parma P.

Journal of Applied Genetics

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2010

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tom 51

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nr 4

EN

Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.

5

In vitro cultures of animal and human cells

86%

Slomski R. , Nowak A. , Hryhorowicz M.

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2015

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tom 96

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nr 1

6

Infertility and marker chromosomes: Application of molecular cytogenetic techniques in a case of inv dupp.89-91,fig.,ref.

86%

Vulcani-Freitas T. M. , Gil-da-Silva-Lopes V. L. , Varella-Garcia M. , Maciel-Guerra A. T.

Journal of Applied Genetics

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2006

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tom 47

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nr 1

EN

We report on a phenotypically normal man with infertility, whose 47,XY,+mar karyotype was studied by spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) using a chromosome-15-specific probe (LSI SNRPN). By these techniques, the marker chromosome was identified as a small inv dup (15). Possible causes for male infertility in this case are discussed.

7

Fluorescence in situ hybridization [FISH] - application in research and diagnostics

86%

Budny B. , Kanik M. , Latos-Bielenska A.

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2002

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tom 40

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nr 2

8

A brief overview of the applicability of FISH methods in environmental engineering

72%

Piórczyk J. , Jasionowski B. , Stańczyk E.

Structure and Environment

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2013

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tom Vol. 5, no. 1

42--45

EN

Fluorescent in situ hybridization (FISH) is a commonly used method for the detection of microorganisms in heterogeneous environment. It is primarily used for quantitative analysis of microorganisms. FISH gives possibilities of evaluating activities of some bacteria and their physiological state in the environment. In the recent years, ftuorescent in situ hybridization has been improved and adapted for the research on environmental samples.

9

BAC-FISH assays delineate complex chromosomal rearrangements in a case of post-Chernobyl childhood thyroid cancer

72%

Kwan J. , Baumgartner A. , LU C.-M. , Wang M. , Weier J. F. , Zitzelsberger H. F. , Weier H.-U. G.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 2

135-144

10

Review of imaging solutions for integrated quantitative mmunohistochemistry in the pathology daily practice

72%

Rojo M. G. , Bueno G. , Slodkowska J.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 3

11

Prokaryotes from different phylogenetic groups in surface microlayer and subsurface water in a eutrophic lake

72%

Burkowska A. , Walczak M.

Polish Journal of Environmental Studies

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2013

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tom 22

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nr 4

EN

Prokaryotes from different phylogenetic groups were studied in surface microlayer (SM, up to 100 μm) and subsurface water (SW – 20 cm) in a eutrophic lake over three months (July, August, and October). The abundance of prokaryotes was determined by epifluorescence microscopy after DAPI staining, and phylogenetic diversity was determined by fluorescence in situ hybridization (FISH) with group-specific, fluorescently labeled oligonucleotide probes. In SW bacteria made up most of the entire community of DAPI-stained microorganisms (54-69%) and in SM bacteria made up only 33-44% of DAPI-stained microorganisms. Archaea corresponded to a small fraction of both bacterioneuston and bacterioplankton. The counts of Archaea and bacteria were significantly higher in SW than in SM. Among all proteobacteria included in the research, γ-proteobacteria represented the most abundant fraction: 42-72% in SM and 39-61% in SW. Statistical analysis revealed that the abundance of γ-proteobacteria is positively correlated with temperature and with dissolved oxygen. β-proteobacteria were the least abundant fraction.

12

Cross-species hybridizations in situ of genes associated with meat production traits in the wild pig genome

72%

Kozubska-Sobocinska A. , Danielak-Czech B. , Babicz M.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

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2015

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tom 33

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nr 3

13

Polymorphism of cytogenetic markers in wild and farm red fox (Vulpes vulpes) populations

72%

Bugno-Poniewierska M. , Solek P. , Potocki L. , Pawlina K. , Wnuk M. , Jezewska-Witkowska G. , Slota E.

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nr 3-4

14

Hepatic tissue changes in rats due to chronic invasion of Babesia microti

72%

Okla H. , Jasik K. P. , Slodki J. , Rozwadowska B. , Slodki A. , Jurzak M. , Pierzchala E.

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2014

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tom 62

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nr 4

15

Application of IGF2 - specific identifier probe for cytogenetic study of somatic and sperm cells in horses

72%

Potocki L. , Bugno-Poniewierska M. , Tischner M. , Wnuk M.

Folia Biologica

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2011

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tom 59

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nr 3-4

16

Conventional and molecular chromosome study in the European genus Parnassiana Zeuner, 1941 (Orthoptera, Tettigoniinae, Platycleidini)

72%

Grzywacz B. , Heller K.-G. , Chobanov D. P. , Warchalowska-Sliwa E.

Folia Biologica

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2017

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tom 65

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nr 1

17

Subtelomeric rearrangements in Polish subjects with intellectual disability and dysmorphic features

72%

Bogdanowicz J. , Pawlowska B. , Ilnicka A. , Gawlik-Zawislak S. , Jozwiak A. , Sobiczewska B. , Zdzienicka E. , Korniszewski L. , Zaremba J.

Journal of Applied Genetics

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2010

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tom 51

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nr 2

215-217

EN

Fluorescent in situ hybridization (FISH) was performed in 76 patients referred to our department because of intellectual disability and dysmorphic features that can be related to subtelomeric microaberrations. In all the patients, conventional cytogenetic methods revealed normal karyotype. Four (5.3%) subtelomeric rearrangements were detected by FISH: 2 subtelomeric 1p36 deletions, an unbalanced translocation involving chromosomes 1 and 12 with 1p36 deletion, and a de novo balanced translocation involving chromosomes 19 and 22. Thus, 3 cases of 1p36 subtelomeric deletion were found (3.95%). To confirm subtelomeric rearrangements in 2 patients, comparative genomic hybridization (CGH) was applied. Moreover, 3 cases of polymorphism without phenotypic effects were found: in 2 patients, the polymorphism involved the long arm of chromosome 2 (maternal derivative in both patients), while in the third patient, a polymorphism of the long arm of chromosome 7 was diagnosed. The latter polymorphism was also found in the patient's mother and grandfather.

18

FISH and GISH analysis of Brassica genomes

72%

Hasterok R. , Ksiazczyk T. , Wolny E. , Maluszynska J.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

EN

Fluorescence and genomic in situ hybridization (FISH and GISH) methods were used for discrimination of Brassica genomes. The three diploid and three allotetraploid species of Brassica, known as the "U-triangle," represent an attractive model for molecular and cytologieal analysis of genome changes during phylogeny in the genus Brassica. The use of genomic DNA probes enabled unambiguous discrimination of the ancestral genomes in B. juncea and B. carinata, and was only partially successful in B. napus. GISH signals in all genomes were localized predominantly in pericentromeric regions of chromosomes. Simultaneous application of genomic and ribosomal DNA probes in multicolor GISH and FISH allowed identification of a significant number of chromosomes in the B. juncea complement. The study also revealed that species of Brassica possess Arabidopsis-type telomeric repeats which in all genomes occupied exclusively terminal, that is, telomeric, locations of chromosomes.

19

Mucosal flora in Crohn’s disease and ulcerative colitis - an overview

58%

Swidsinski A. , Loening-Baucke V. , Herber A.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 6

20

Polymorphism of the rDNA chromosomal regions in the weatherfish Misgurnus fossilis (Teleostei: Cobitidae)

58%

Spoz A. , Boron A. , Ocalewicz K. , Kirtiklis L.

Folia Biologica

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2017

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tom 65

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nr 1