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EN
Aerobic and anaerobic bacterial flora of post-operative incisions with drainege were exam­ined. From each of 28 patients three specimens were taken; during operation (smear from peritoneal cavity), liquid from drain (taken at 3-th day after operation) and smear from drain taken at the end of drainage. Enterococci, Enterobacteriaceae spp. and anaerobes, especially Bactericides spp. were most often isolated from specimens taken during operation. Enterococci and caogulasenegative Staphylococci - often resistant to methicillin, were most often isolated from specimens taken at the end of drainage.
PL
Przeprowadzono szczegółową identyfikację flory bakteryjnej ran pooperacyjnych u pacjentów poddanych chirurgicznemu leczeniu raka krtani oraz oceniono jej wpływ na proces gojenia. W analizie uwzględniono rozległość procesu nowotworowego, stan jamy ustnej i uzębienia oraz zastosowaną antybiotykoterapię pooperacyjną. Na podstawie uzyskanych wyników stwierdzono, że występowanie powikłań ropnych wiąże się z kolonizacją ran egzogenną florą Gram-dodatnią.
EN
Finding of the a etiologic factors and participation of bacteria flora in wound healing in laryngeal cancer treatment was the purpose of our study. Investigations were performed in 27 patients. Swabs were taken from the postoperative wounds. Detiled identifications of the bacteria flora and antibiotic susceptibility of bacteria were performed. Wound healing was estimated according to extension of the carcinoma, applied antibiotics, stale of the oral cavity, the kind of bacteriological flora isolated after surgical treatment from postoperative wounds. It was found that wound healing depended on the extension of carcinoma, as well as, type of isolated bacteria and antibiotic therapy used. The proper healing of postoperative wounds was not dependent on the state of the oral cavity and the dentition. The main cause of postoperative complication of wounds was methicillin-resistant Staphylococcus aureus (MRSA).
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tom 62
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nr 10
1116-1119
EN
Similar bacterial floras are present in the vagina and uterus of healthy bitches. The most common of these bacteria are b-hemolytic streptococci, Pasteurella multocida and E. coli and Staphylococcus intermedius. Bacterial flora varies during the estrous cycle with the highest bacterial count occurring during heat. E. coli was most frequently isolated from bitches with pyometra. No specific bacterial species are associated with vaginitis and infertility.
EN
Neutral mine drainage is the less frequent subject of the interest than acid mine drainage but it can have adverse environmental effects caused mainly by precipitation of dissolved Fe. The aim of the study was to characterize the composition of bacterial population in environment with high concentration of iron and sulfur compounds represented by neutral mine drainage water of Elizabeth’s shaft, Slovinky (Slovakia). The pH value of drainage water decreased from 7.1 to 6.5 during the years 2008–2014. Direct microscopic observations, cultivation methods, and 454 pyrosequencing of the 16S rRNA gene amplicons were used to examine the bacterial population. Microscopic observations identified iron–oxidizing Proteobacteria of the genera Gallionella and Leptothrix which occurrence was not changed during the years 2008–2014. Using 454 pyrosequencing, there were identified members of 204 bacterial genera that belonged to 25 phyla. Proteobacteria (69.55%), followed by Chloroflexi (10.31%) and Actinobacteria (4.24%) dominated the bacterial community. Genera Azotobacter (24.52%) and Pseudomonas (14.15%), followed by iron–oxidizing Proteobacteria Dechloromonas (11%) and Methyloversatilis (8.53%) were most abundant within bacterial community. Typical sulfur bacteria were detected with lower frequency, e.g., Desulfobacteraceae (0.25%), Desulfovibrionaceae (0.16%), or Desulfobulbaceae (0.11%). Our data indicate that the composition of bacterial community of the Elizabeth’s shaft drainage water reflects observed neutral pH, high level of iron and sulfur ions in this aquatic habitat.
PL
Odciek kopalniany o odczynie obojętnym jest rzadszym przedmiotem zainteresowania niż odciek kopalniany kwaśny, ale może mieć niekorzystne skutki środowiskowe spowodowane głównie przez wytrącanie rozpuszczonego Fe. Celem artykułu jest scharakteryzowanie składu bakterii w środowisku o wysokim stężeniu związków żelaza i siarki reprezentowanych przez obojętne wody drenażowe kopalni szybu Elizabeth, Slovinky (Słowacja). Wartość pH wody drenażowej spadła z 7,1 do 6,5 w latach 2008–2014. Bezpośrednie obserwacje mikroskopowe, metody hodowli i pirosekwencjonowanie amplikonów genu 16S rRNA wykorzystano do zbadania populacji bakterii. Obserwacje mikroskopowe zidentyfikowały proteobakterie utleniające żelazo z rodzajów Gallionella i Leptothrix, których występowanie nie uległo zmianie w latach 2008–2014. Przy użyciu pirosekwencjonowania 454 zidentyfikowano 204 rodzajów bakterii należących do 25 typów. Proteobakterie (69,55%), a następnie Chloroflexi (10,31%) i aktynobakte rie (4,24%) zdominowały społeczność bakteryjną. Rodzaje Azotobacter (24,52%) i Pseudomonas (14,15%), a następnie proeto-bakterie żelazo utleniające Dechloromonas (11%) i Methyloversatilis (8,53%) były najbardziej rozpowszechnione w społeczności bakteryjnej. Typowe bakterie siarkowe wykryto z mniejszą częstotliwością, np. Desulfobacteraceae (0,25%), Desulfovibrionaceae (0,16%) lub Desulfobulbaceae (0,11%). Uzyskane dane wskazują, że skład flory bakteryjnej wody drenażowej szybu Elżbieta odzwierciedla obserwowane neutralne pH, wysoki poziom zawartości jonów żelaza i siarki w środowisku wodnym.
EN
After slaughter or surgically there were taken oviducts from 16 cows, 28 ewes and 7 does to assess their contents bacteriologically. It was found that the oviducts taken from cows and does were free from bacterial flora in 56, 58 and 100 per cent, respectively. Streptococcus spp was found in 6 cases and Staphylococcus spp in 7 cases in cows. In ewes Streptococcus spp was noted in 5, Staphylococcus in 13 and Pseudomonas spp in one cause. There was no growth of opportunistic bacteria in the rinsings of the oviduct of does.
PL
Ciało człowieka i innych zwierząt zamieszkuje bogata endogenna mikro-biota, w większości bardzo slabo poznana, odgrywająca kluczową rolę w prawidłowym ich funkcjonowaniu. Większość zwierząt odżywiających się celulozą, krwią, sokami roślinnymi, mających zdolność bioluminescencji, korzysta z usług niewidocznych sojuszników.
EN
The body of man or other animals is inhabited by the rich endogenous microbiota, most of them very poorly understood and fulfills a key role in their proper functioning. Most of the animals that feed on cellulose, blood, plant juices, have the ability to bioluminesce based on the services of invisible allies.
EN
The aim of this study was to compare the bacterial flora of canine vagina and uterus and assess its composition during the estrus cycle. From 28 healthy bitches, vaginal smears and samples of uterine secretion by means of endoscopy were collected during the estrus cycle (anestrus, proestrus, estrus, metestrus) for bacteriological investigation. Similar bacterial flora were present in the vagina and uterus of bitches. Lactobacillus sp.,α-hemolytic streptococci, Staphylococcus intermedius, Pasteurella multocida and E. coli were most frequently found. The average number of isolates was 2.2 per vaginal sample and 1.4 per uterine sample, the uterine samples also showed a lower bacterial count. The composition of vaginal and uterine bacterial flora and amounts of bacteria varied during the estrus cycle. The highest bacterial count occurred during proestrus.
EN
The aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. Aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). Mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). The isolated aerobic bacteria were as follows: streptococci from the species Streptococcus salivarius, Streptococcus anginosus, Streptococcus group C, Streptococcus sanguis, Staphylococcus aureus, Gram-negative rods from the genus Haemophilus and rods from the Enterobacteriaceae family, and strains of Moraxella catarrhalis. The isolated anaerobic microorganisms Gram-negative rods from the genus Prevotella, Bacteroides, Fusobacterium, Gram-positive cocci from the genus Peptostreptococcus. Other organisms from the genus Vailonella, Eubacterium and Actinomyces were isolated less frequently. In 15 patients only one isolate was recovered, in 15 patients isolated bacteria were mixed with other microorganisms.
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