Wyniki wyszukiwania - Biblioteka Nauki

1

Preliminary evaluation of selected biologic properties of TiO2 and SiO2 layers on metallic substrates

100%

Urbański W. , Dragan S. , Gębarowska E. , Dzięgiel P. , Krzak-Roś J. , Miller M. , Pezowicz C. , Będziński R.

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2010

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tom Vol. 13, no. 96-98

129--133

EN

Despite of applying modern biomaterials during constructing long term orthopaedic implants, in clinical practice there are still present wide range of complications, particularly concerning matter of implant - tissue interactions. Since interaction between implant and living tissue depends mainly on biomaterial surface features, we decided to modify orthopaedic alloys to improve their biological properties. The object of this experiment was in vitro evaluation of selected biological properties, particularly cytotoxicity of titanium alloy and 316L stainless steel substrates coated with SiO2 or TiO2 thin films. The coatings were synthesized by sol-gel method. Each samples was placed into mouse fibroblast culture. The cultures in presence of tested materials were maintained for three days. We found no distinct toxic effect of tested biomaterials. We noticed increase of fibroblast proliferation in cultures with uncoated titanium and particularly SiO2 coated titanium plates.

2

Nichrome Capacitors on Polycarbonate Substrate for Monitoring Cell Culture Using Impedance Sensing Technique

88%

Kociubiński A. , Zarzeczny D. , Prendecka M. , Pigoń D. , Małecka-Massalska T.

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2020

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tom Vol. 65, iss. 1

493--496

EN

The aim of this work was to present a method of tissue culture research by measuring the impedance of cells cultured in the presence of nichrome. For this purpose, the Electric Cell-substrate Impedance Sensing system was used with a prototype substrate containing comb capacitors made of nichrome. Magnetron sputtering, photolithography and etching processes were used to produce the thin-film electrodes. In the experimental part, cells of mouse fibroblast cell line L929 were cultured according to the instruction manual in complete medium, under controlled growth conditions. Inoculation of arrays was carried out by 300 microliters per well of cell suspension at ~1.2×105 cells/ml. The results of the monitoring cells behavior in tissue culture indicate good cell viability and proliferative potential.

3

Morphological and biochemical changes in human fibroblast lines induced by anthracyclines during apoptosis

88%

Kania K. , Dragojew S. , Jozwiak Z.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

Involvement of epithelial-mesenchymal transition-inducing transcription factors in the mechanism of Helicobacter pylori-induced fibroblasts activation

75%

Krzysiek-Maczka G. , Targosz A. , Szczyrk U. , Strzalka M. , Brzozowski T. , Ptak-Belowska A.

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nr 5

5

The usefulness of ultrastructure investigations for the evaluation of the vitality of aortal homografts

75%

Czarnowska E. , Maruszewski B. , Fedorowicz M.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 4

6

Nichrome Capacitors on Polycarbonate Substrate for Monitoring Cell Culture Using Impedance Sensing Technique

75%

Kociubiński A. , Zarzeczny D. , Prendecka M. , Pigoń D. , Małecka-Massalska T. , Kociubiński A. , Zarzeczny D. , Prendecka M. , Pigoń D. , Małecka-Massalska T.

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7

The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

75%

Galanty A. , Michalik M. , Sedek L. , Podolak I.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 4

585-598

EN

We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.

8

Influence of vitamin D3 analogues in combination with budesonid R on proliferation of nasal polyp fibroblasts

75%

Rostkowska-Nadolska B. , Frączek M. , Gawron W. , Latocha M.

Acta Biochimica Polonica

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2009

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tom 56

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nr 2

235-242

EN

Vitamin D (VD) and its different analogues, besides their classic role as regulators of calcium and phosphor homeostasis, have emerged as a large family of antiproliferative agents. Such properties suggested VD potential as a therapy for chronic inflammatory diseases, including nasal polyposis (NP). NP growth involves both an inflammatory process and the proliferation of fibroblast as an important factor inducing aberrations in the phenotype of the epithelium. The aim of this study was to investigate the possible influence of 1α,25-dihydroxyvitamin D3 (calcitriol) and 1α,24(R)-dihydroxyvitamin D3 (tacalcitol) in monotherapy and in combination with budesonid R (BR) on NP fibroblast proliferation. Material and methods: The study involved 26 samples of NP. NP cells were cultured on 96-well plates beginning with a concentration of 5 × 103 cells per well with RPMI 1640 medium supplemented with antibiotics and 10% foetal bovine serum. After the fourth to sixth passage the medium was replaced with a nutrient medium with calcitriol or tacalcitol in a defined concentration (from 10-9 M to 10-3 M) alone or in combination with BR in 1:1, 1:3 or 3:1 ratios, each at concentrations from 10-5 M to 10-3 M. Results: Growth inhibition of nasal fibroblasts exposed to calcitriol or tacalcitol was noted. Significant antiproliferating activity was observed at calcitriol concentrations of 10-4 M and 10-3 M after 48 h, and at a concentration of 10-3 M after 72 h with the percentage of proliferating cells reduced to 30% compared to the control samples (P < 0.05). In cells treated with tacalcitol the maximal effect was seen at 10-4 M after 48 h and at 10-3M after 72 h with a 60% inhibition with respect to the control (P < 0.05). The inhibition of fibroblast proliferation reached the maximal level when they were exposed to calcitriol: BR (1 : 1) or tacalcitol: BR (1 : 1), each at a concentration of 10-4 M, after 72 h (82% and 69%, respectively). Conclusions: The antiproliferative activity of calcitriol and tacalcitol in NP cultures was confirmed. Because of its lower toxicity and higher activity tacalcitol seems to be the more promising agent in NP therapy, both as a single medication and in treatment protocols with BR.

9

Insulin influence on glycosaminoglycan synthesis in diabetic [IDDM] fibroblasts

75%

Yevdokimova N.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

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nr 2

10

F-actin disrupting agents affect the fluid-phase endocytosis of human skin fibroblasts in culture

75%

Michalik M. , Wianecka-Skoczen M. , Korohoda W.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

11

Influence of kinetin [6-furfurylo-amino-purine] on human fibroblasts in the cell culture

75%

Kowalska E.

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tom 51

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nr 2

12

High density lipoprotein and low density lipoprotein-mediated changes in cultured human skin fibroblasts

75%

Jozwiak Z. , Szosland D. , Rozga B.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 2

EN

We investigated the effect of native and modified high density lipoprotein (HDL) and low density lipoprotein (LDL) on the viability and the membrane fluidity of normal and diabetic fibroblasts. Under experimental conditions, HDL did not affect the cultured normal cells. Incubation of HDL with diabetic fibroblasts resulted in a negligible decrease in the cell viability and induced an increase in the lipid fluidity at surface of the cell membranes. LDL at the higher concentration reduced the viability both normal and diabetic fibroblasts and also enhanced the fluidity of membrane lipids. The above changes in the fluidity of fibroblast membranes were observed mainly in the presence of native and oxidized lipoproteins and are connected with the differences in the structure of cell membranes and nature of the cytotoxicity of LDL and HDL.

13

The effect of melatonin on antioxidant enzymes in human diabetic skin fibroblasts

75%

Kilanczyk E. , Bryszewska M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

14

Uterine myofibroblasts in mares

75%

Sabeckiene J. , Van Ederen A. M. , Zeinstra E. , Aniuliene A. , Gruys E.

Medycyna Weterynaryjna

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2005

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tom 61

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nr 12

15

Spreading-independent growth of normal fibroblasts in three-dimensional cultures

75%

Czyz J. , Rajwa B. , Bzowska M. , Dobrucki J. , Korohoda W.

Folia Biologica

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2004

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tom 52

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nr 1-2

16

The use of flow cytometry for the investigation of viability of heart valve - derived fibroblasts

75%

Wilczek P. , Szydlowska I. , Lotysz D. , Religa Z.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

17

The modulation of transferrin receptors level on mouse macrophages and fibroblasts by Toxoplasma gondii

75%

Dziadek B. , Dytnerska-Dzitko K. , Dlugonska H.

Polish Journal of Microbiology

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2004

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tom 53

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nr Suppl.

EN

Macrophage-mediated early nonspecific immunological response is an important part of the immunity against intracellular parasite Toxoplasma gondii. The immunological functions of macrophages are closely connected with iron metabolism and acquiring of iron mainly from transferrin by the receptor-mediated endocytosis. The level of specific transferrin receptors can be modulated by different soluble exogenous and endogenous factors and also by microbial pathogens. The goal of our study was to determine the influence of T. gondii infection and toxoplasma lysate antigen (TLA) on the expression level of transferrin receptors (TfRs) on mouse macrophages and fibroblasts which can serve as host cells for the parasite replication. The level of TfRs was measured using CELISA assay. Strong down-regulation of the receptors level, started about 18 hours after infection of macrophages with a high number of freshly harvested tachyzoites T. gondii. Stimulation of the mouse cells with TLA antigen did not cause any changes in TfRs expression. In our studies we did not observe any differences in the TfRs level on mouse fibroblasts even after incubation with high concentrations of TLA antigen or inoculation with a high number of tachyzoites.

18

Mitochondrial heat shock response induced by ectromelia virus is accompanied by reduced apoptotic potential in murine L929 fibroblasts

63%

Wyzewski Z. , Gregorczyk-Zboroch K. P. , Mielcarska M. B. , Bossowska-Nowicka M. , Struzik J. , Szczepanowska J. , Toka F. N. , Niemialtowski M. G. , Szulc-Dabrowska L.

Archivum Immunologiae et Therapiae Experimentalis

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2019

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tom 67

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nr 6

19

Inhibition of fibroblast apoptosis by Borrelia afzelii, Coxiella burnetii and Bartonella henselae

63%

Chmielewski T. , Tylewska-Wierzbanowska S.

Polish Journal of Microbiology

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2011

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tom 60

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nr 3

EN

Apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis. The aim of this study was to investigate the influence of Borrelia afzelii, Coxiella burnetii, and Bartonella henselae bacteria on apoptosis measured as the level of caspase 3 activity in human fibroblast cells HEL-299. Our findings show that C. burnetii bacteria may inhibit the process of apoptosis in the host cells for a long time. This can permit intracellular survival in the host and mediatingthe development of chronic disease.

20

The induction of apoptosis by daunorubicin and idarubicin in human trisomic and diabetic fibroblasts

63%

Dragojew S. , Marczak A. , Maszewski J. , Ilnicki K. , Jozwiak Z.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 2

182-194

EN

In this study, we investigated apoptosis induced in human trisomic and diabetic fibroblasts by daunorubicin (DNR) and its derivative, idarubicin (IDA). The cells were incubated with DNR or IDA for 2 h and then cultured in a drug-free medium for a further 2–48 h. The apoptosis in the cultured cell lines was assessed by biochemical analysis. We found that both drugs induced a timedependent loss of mitochondrial membrane potential, and a significant increase in intracellular calcium and caspase-3 activity. Mitochondrial polarization and changes in the level of intracellular calcium were observed during the first 2–6 h after drug treatment. Caspase-3 activation occurred in the late stages of the apoptotic pathway. Our findings also demonstrated that idarubicin was more cytotoxic and more effective than daunorubicin in inducing apoptosis in trisomic and diabetic fibroblasts.