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Attempt at regeneration of Pharbitis nil from fragments of vegetative organs

100%

Trejgell A. , Tretyn A. , Nicos D.

Acta Physiologiae Plantarum

1998

tom 20

nr 2

The object of study was the regeneration of Pharbitis nil by direct and indirect organogenesis. From fragments of roots, cotyledons, hypocotyls and epicotyls on Murashige and Skoog nutrient solution (MS) supplemented with naphtalenacetic acid (NAA) or indolylacetic acid (IAA; both at 0.1 mg·dm⁻³ concentration) in the presence of benzylaminopurine (BAP), zeatin or kinetin (all at 5 mg·dm⁻³ concentration) only root organogenesis was obtained. Likewise, when using the two-step method (2 or 5 days exposure to NAA or IAA at 2 mg·dm⁻³ concentration followed by exposure to BAP or zeatin at 1 or 2 mg·dm⁻³ concentration) root organogenesis was observed in all types of explants. Moreover, shoot buds were formed on fragments of epicotyl exposed vertically in relation to the medium. However, attempts at regenerating complete plants from them failed, so did the regeneration of P. nil from callus. The roots were formed in callus cultures only.

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

84%

Huang B. , Chu C. , Chen S. , Juan H. , Chen Y.

Cellular and Molecular Biology Letters

2006

tom 11

nr 2

Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.