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Amino acid 106 in human cytosolic thymidine kinase is a valine, and is important for the oligomerisation and enzymatic properties

100%

Berenstein D. , Christensen J. F. , Frederiksen H. , Kristensen T. , Munch-Petersen B.

Cellular and Molecular Biology Letters

1999

tom 04

nr 3

Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases

84%

Wlodawer A. , Li M. , Gustchina A. , Oyama H. , Dunn B. M. , Oda K.

nr 1

Sedolisins (serine-carboxyl peptidases) are proteolytic enzymes whose fold resembles that of subtilisin; however, they are considerably larger, with the mature catalytic domains containing approximately 375 amino acids. The defining features of these enzymes are a unique catalytic triad, Ser-Glu-Asp, as well as the presence of an aspar- tic acid residue in the oxyanion hole. High-resolution crystal structures have now been solved for sedolisin from Pseudomonas sp. 101, as well as for kumamolisin from a thermophilic bacterium, Bacillus novo sp. MN-32. The availability of these crystal structures enabled us to model the structure of mammalian CLN2, an enzyme which, when mutated in humans, leads to a fatal neurodegenerative disease. This review compares the structural and enzymatic properties of this newly defined MEROPS family of peptidases, S53, and introduces their new nomenclature.

Soil lipolytic activity under the cultivation of Salix viminalis L. enriched with sewage sludge. Part I

67%

Furczak J. , Joniec J. , Baran S.

nr 1

Studies performed in a field indicate that podzolic soil combined with sewage sludge under Salix viminalis L. cultivation exhibited an increase in lipolytic bacteria and fungi numbers as well as a clear stimulation of lipase activity at level A. These changes increased in accordance with the increase of sludge concentration in the soil, and remained in the second and the third year of the experiment. However, a decrease of lipase activity was observed in subjects with a higher sludge concentration in the third year. Disturbances of microbial and enzymatic soil properties after the addition of sewage sludge occurred less intensively at the 20-40 cm than the 0-20 cm layer. In general, a slight stimulation of lipolytic bacteria and fungi and a stronger stimulation of lipase activity was found with higher doses of sludge. As at level A, these changes remained active for two years, showing a decline of lipase activity in the third year.

Selected enzymatic properties of bacterioplankton in lakes of various degrees of eutrophication

59%

Wcislo R. , Chrost R. J.

nr 2