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  1. 15 Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
  2. 10 Polish Journal of Food and Nutrition Sciences
  3. 9 Przemysł Chemiczny
  4. 7 Chemical Engineering and Equipment
  5. 7 Inżynieria i Aparatura Chemiczna
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  1. 3 2023
  2. 8 2021
  3. 8 2020
  4. 3 2019
  5. 4 2018
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  1. 14 Zawadzki J.
  2. 13 Antczak A.
  3. 8 Akus-Szylberg F.
  4. 8 Marchwicka M.
  5. 8 Radomski A.
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1
Content available Hydrothermal pretreatment of poplar (Populus trichocarpa) wood and its impact on chemical composition and enzymatic hydrolysis yield
100%
Akus-Szylberg F. , Antczak A. , Zawadzki J.
Drewno : prace naukowe, doniesienia, komunikaty
|
2020
|
tom vol. 63, nr 206
5--18
EN
This paper focuses on the effect of liquid hot water pretreatment of fast-growing poplar wood in the context of bioethanol production. The milled Populus trichocarpa wood with a particle size of 0.43-1.02 mm was pretreated with liquid hot water method at temperatures range from 160ºC to 205ºC and then was subjected to enzymatic hydrolysis. The glucose and xylose content in the hydrolyzates were analyzed with high performance liquid chromatography. On the basis of results it was concluded, that increase of temperature in the hydrothermal pretreatment resulted in an increase of glucose and decrease of xylose content. However, increased temperature of the process led to inhibitor formation.
2
Synthesis of P-chiral, non-racemic phosphinylacetates via enzymatic resolution of racemates
100%
Kiełbasiński P. , Żurawiński R. , Pietrusiewicz K. M. , Zabłocka M. , Mikołajczyk M.
Polish Journal of Chemistry
|
1998
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tom Vol. 72, nr 3
564-572
EN
A series of racemic methyl phosphinylacetates was hydrolyzed in the presence of porcine liver esterase (PLE) under the kinetic resolution conditions to give the corresponding P-chiral phosphinylacetic acids and recovered esters in high enantiomeric purity (72-100%ee). The Jones' active site model was applied to explain the enantioselectivity of the reaction.
3
Content available Change in hydrolytic enzyme efficiency over time
100%
Szadkowska D.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
|
2020
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tom No. 112
79--84
EN
Change in hydrolytic enzyme efficiency over time. The purpose of this study was to determine the action of hydrolytic enzymes (by Dyadic Cellulase CP CONC, and the Dyadic Xylanase 2 XP CONC) over time. Chromatographic analysis of holocellulose samples subjected to enzymatic hydrolysis was performed. The following hydrolysis parameters were used: time 48h, temperature 45 ⁰C, acetate buffer pH 5.4, commercial enzymes Dyadic. Holocellulose extracted by the sodium chlorite method from white poplar wood (Populus alba L.) was used. The final yield of enzymatic hydrolysis was determined. The results of hydrolysis performed at intervals were compared. The results obtained show that the hydrolysis yield of holocellulose after five months decreased by 40 p.p. for glucose yield and by 25 p.p. for xylose yield. The yield for glucose after two and a half years decreases by 68 p.p. and 62 p.p. for xylose compared to the initial yield.
PL
Zmiana wydajności enzymów hydrolitycznych w czasie Celem pracy było określenie działania enzymów hydrolitycznych w czasie. Przeprowadzono analizę chromatograficzną próbek holocelulozy poddanych hydrolizie enzymatycznej. Zastosowano następujące parametry hydrolizy: czas 48h, temperatura 45 ⁰C, bufor octanowy pH 5,4, enzymy komercyjne Dyadic. Zastosowano holocelulozę pozyskaną metodą chlorynu sodowego z drewna topoli bialej (Populs alba L.). Określono końcową wydajność hydrolizy enzymatycznej. Porównano wyniki hydrolizy przeprowadzonej w odstępach czasu. Uzyskane wyniki wskazują, że wydajność hydrolizy holocelulozy po pięciu miesiącach zmniejszyła się o 40 p.p. dla wydajności glukozy i o 25 p.p. dla wydajności ksylozy. Wydajność glukozy po dwóch i pół roku obniża się o 68 p.p., a ksylozy o 62 p.p. w stosunku do wydajności początkowej.
4
Content available remote Determination of the aglycon moieties of glycosidically bound compounds in Flos Chrysanthemi by GC × GC–TOFMS
88%
Zhou Y. , Huang G. , Li X. , Chen F. , Liu H. , Yang Y. , Fan Z. , Jiang J. , Yang J.
Acta Chromatographica
|
2018
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tom Vol. 30, no. 3
195--199
EN
A credible method for determination of the aglycon moieties of glycosidically bound aroma compounds in Flos Chrysanthemi by comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) has been proposed. The aglycon moieties of glycosidically bound aroma compounds were isolated using methyl-tert-butyl ether (MTBE) extraction following enzymatic hydrolysis. The GC × GC–TOFMS analysis was performed to comprehensively identify different forms of the released aroma components in Flos Chrysanthemi. The result shows that the limit of detection of the released aglycon moieties ranged from 0.3 to 3.1 ng/mL, the recovery of the released 1-octanol was better than 98.3%, and the intra-day and inter-day precisions of this method were 0.2 to 8.9% and 1.3 to 9.1%, respectively. The proposed method was applied to the analysis of four types of Flos Chrysanthemi (Chuju, Boju, Hangju, and Gongju). A total of 60 aglycon moieties of interest were identified in the four types of Flos Chrysanthemi. These aglycones mainly consisted of aliphatic, aromatic, monoterpene, C13-norisoprenoids, and miscellaneous compounds.
5
Content available The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production
88%
Smuga-Kogut M. , Bugajski S. , Piskier T. , Walendzik B.
Rocznik Ochrona Środowiska
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2023
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tom Tom 25
413--422
EN
The study aimed to obtain bioethanol from biomass using chemical treatment and enzymatic hydrolysis. Different concentrations of sodium hydroxide (5 and 10%) were used for the delignification process, and enzymatic hydrolysis was carried out using three commercial cellulolytic preparations (Cellic® CTec2, cellulase from Trichoderma reesei and cellulase from Aspergillus species). The final step involved an alcoholic fermentation process using Saccharomyces cerevisiae TYPE II yeast. After enzymatic hydrolysis, the content of reducing sugars was determined in the samples, and the fermentation yield was controlled by determining the ethanol content by pycnometry. Using chemical pretreatment increased the yield of the whole process by at least 50%. The content of reducing sugars after hydrolysis depended on the type of enzyme preparation used for hydrolysis and the use of NaOH in pretreatment. The highest reducing sugars content (45.8 g/dm3) was achieved in a sample of material purified with 5% NaOH, and enzymatic hydrolysis was carried out using Cellic® CTec2. It means the efficiency of the enzymatic hydrolysis process equals 94.69%. The concentration of bioethanol after alcoholic fermentation in this sample was 0.509 g/l.
6
Content available Application of x-ray fluorescence technique for determination of heavy metals uptake by different species of poplar
88%
Zielenkiewicz T. , Szadkowski J. , Drożdżek M. , Zielenkiewicz A. , Kłosińska T. , Antczak A. , Zawadzki J. , Gawron J.
Drewno : prace naukowe, doniesienia, komunikaty
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2016
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tom vol. 59, nr 197
113--126
EN
X-ray fluorescence (XRF) analysis of six metals: chromium, manganese, iron, nickel, copper and zinc (possible inhibitors of enzymatic hydrolysis of wood) in samples of two poplar species, Populus trichocarpa and Populus maximowiczii, was performed in order to check which of them collect more of the metallic inhibitors during tree growth and steam explosion pretreatment. The XRF point scan (for solid and ashed wood) and mapping (for stem cross-sections) options were used. Samples of the different parts – stem, branches, leaves and bark were studied. Steam explosion at 130°C, 160°C and 190°C was performed on both species and the influence of steam on the chosen metals content was analysed. On the basis of the results, P. trichocarpa is the species which accumulates a higher amount of the metals during tree growth and P. maximowiczii – during steam explosion.
7
Content available Alkaline Pre-Treatment and Enzymatic Hydrolysis of Waste Papers to Fermentable Sugar
88%
Ojewumi M. E. , Obielue B. I. , Emetere M. E. , Awolu O. O. , Ojewumi E. O.
Journal of Ecological Engineering
|
2018
|
tom Vol. 19, nr 1
211--217
EN
Waste paper is known to be the major component of organic solid waste. In this research, waste paper was used as a feedstock for the production of fermentable sugar with the aid of two (2) microorganisms. The waste papers used included newspaper, office paper and foolscap paper. Enzymatic hydrolysis was carried out on the waste papers after the alkaline treatment using Aspergillus niger and Pseudomonas aeruginosa at different temperatures of 25°C, 37°C and 42°C. The highest yield was obtained from the foolscap paper, which produced reducing sugar at a maximum concentration of 486.66 mg/L after two weeks using Pseudomonas aeruginosa at 37°C. On the other hand, hydrolysing using Aspergillus niger, produced reducing sugar at a maximum concentration of 365 mg/L at an optimum temperature of 25°C with office paper.
8
Content available The comparison of acid and enzymatic hydrolysis of pulp obtained from poplar wood (Populus sp.) by the Kraft method
88%
Antczak A. , Świerkosz R. , Szeniawski M. , Marchwicka M. , Akus-Szylberg F. , Przybysz P. , Zawadzki J.
Drewno : prace naukowe, doniesienia, komunikaty
|
2019
|
tom vol. 62, nr 203
53--66
EN
This paper compares the acid and enzymatic hydrolysis in relation to bioethanol production. The pulp obtained from wood of the fast-growing poplar species (Populus deltoides x maximowiczii and Populus trichocarpa Torr. & A. Gray ex Hook) was used as a feedstock. The delignification process by the Kraft method was carried out with 19% and 26% of active alkali (NaOH and Na2S). The obtained sugars (xylose and glucose) were analyzed by a high-performance liquid chromatography. The results concluded that the enzymatic hydrolysis process is better than acid hydrolysis because a higher content of sugars (especially xylose) was obtained. Additionally, after the acid and enzymatic hydrolysis process from Populus trichocarpa pulp, more sugars were obtained than from the pulp of Populus deltoides × maximowiczii. The Kraft pulp from the wood of fast-growing poplar species is a good raw material for the production of glucose. However, in order to obtain a higher xylose content and finally increase the profitability of bioethanol production, a new method should be developed, or the Kraft method should be optimized.
9
Content available Production of ethanol from wheat straw
88%
Smuga-Kogut M. , Wnuk A. D. , Zgórska K. , Kubiak M. S. , Wojdalski J. , Kupczyk A. , Szlachta J. , Luberański A.
Polish Journal of Chemical Technology
|
2015
|
tom Vol. 17, nr 3
89--94
EN
This study proposes a method for the production of ethanol from wheat straw lignocellulose where the raw material is chemically processed before hydrolysis and fermentation. The usefulness of wheat straw delignification was evaluated with the use of a 4:1 mixture of 95% ethanol and 65% HNO3 (V). Chemically processed lignocellulose was subjected to enzymatic hydrolysis to produce reducing sugars, which were converted to ethanol in the process of alcoholic fermentation. Chemical processing damages the molecular structure of wheat straw, thus improving ethanol yield. The removal of lignin from straw improves fermentation by eliminating lignin’s negative influence on the growth and viability of yeast cells. Straw pretreatment facilitates enzymatic hydrolysis by increasing the content of reducing sugars and ethanol per g in comparison with untreated wheat straw.
10
Content available Bioconversion of Waste Foolscap and Newspaper to Fermentable Sugar
88%
Ojewumi M. E. , Kolawole O. E. , Oyekunle D. T. , Taiwo O. S. , Adeyemi A. O.
Journal of Ecological Engineering
|
2019
|
tom Vol. 20, nr 4
35--41
EN
The aim of this project work was to evaluate the effect of bacteria – Serratia in the enzymatic hydrolysis of Foolscap [FS] and Newspaper [NP] into fermentable or reducing sugars. The effect of temperature and hydrolysis time (number of days) on the extent of concentration of reducing sugar yield were assessed and quantified using Dinitrosalicylic acid test method (DNS). A proximate analysis was carried out on the substrate before hydrolysis. Alkaline pre-treatment using sodium hydroxide and deinking process for the removal of paper with ink for higher susceptibility of the substrate before the enzymatic hydrolysis were carried out. The temperature effect on the waste sample were analysed at 37°C, 40°C and 45°C for 7 days, it was observed that at 37°C, saccharification was higher than at 40°C and 45°C. The general observation was in that Serratia had a very good effect on the waste paper samples. A 3-D surface plot revealed that the yield of sugar increased along with the hydrolysis time (number of days), with Foolscap having the highest yield of about 21mg/ml. The comparative analysis shows that the highest yield was obtained at the temperature of 37°C, for both substrates used.
11
Content available Effect of hydrothermal depolymerization and enzymatic hydrolysis of Miscanthus giganteus biomass on the yield of methane fermentation
88%
Zieliński M. , Dębowski M. , Krzemieniewski M. , Kupczyk K. , Rokicka M. , Grala A. , Hajduk A.
Technical Sciences / University of Warmia and Mazury in Olsztyn
|
2014
|
tom nr 17(3)
209--218
EN
The present study determined the effect of preliminary hydrothermal depolymerization and enzymatic hydrolysis of Miscanthus giganteus biomass on the yield of methane fermentation in terms of the quantity and composition of biogas produced. Enzymatic hydrolysis of the substrate led to an increase in the volume of biogas produced from 0.12 dm3/g substrate in the samples without enzymes to 0.17 dm3/g substrate in variant I, as well as a significant increase in methane. In addition, there were noticeable decreases in dry matter content in all variants to which the enzymatic multicomplex had been added.
12
Content available Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover
88%
Akus-Szylberg F. , Antczak A. , Zawadzki J.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
|
2021
|
tom No. 115
29--36
EN
Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover. The aim of this research was to investigate the effect of applying two different temperatures of the soaking aqueous ammonia treatment on the chemical composition and enzymatic hydrolysis yield of the corn stover. Native corn stover as well as solid fractions after 20 h of alkali pretreatment performed at 15% ammonia solution and at 50 °C or 90 °C were analysed in terms of cellulose, holocellulose, lignin and extractives content. Both untreated and treated samples were subjected to the enzymatic hydrolysis and hydrolysates were examined with a high performance liquid chromatography (HPLC). Results indicated a significant development of enzymatic digestibility of the SAA treated biomass. Furthermore, a 38.7% and a 68.9% delignification levels in the biomass treated with ammonia at respectively 50 °C and 90 °C process comparing to the raw material were achieved.
PL
Wpływ wstępnej obróbki słomy kukurydzianej w wodnym roztworze amoniaku (SAA) na jej skład chemiczny i hydrolizę enzymatyczną. Celem badań było zbadanie wpływu zastosowania dwóch rożnych temperatur podczas obróbki wstępnej wodnym roztworem amoniaku słomy kukurydzianej na jej skład chemiczny oraz wydajność hydrolizy enzymatycznej. Próbki słomy natywnej, jak i słomy po 20 godzinach obróbki w 15% roztworze amoniaku i temperaturze 50 °C lub 90 °C zostały przebadane pod kątem zawartości celulozy, hemiceluloz, ligniny i substancji ekstrakcyjnych. Zarówno materiał natywny, jak i po obróbce został podany również hydrolizie enzymatycznej, a hydrolizaty zostały przebadane przy pomocy wysokosprawnej chromatografii cieczowej (HPLC). Uzyskane wyniki pozwoliły stwierdzić wysoce zwiększoną zawartość cukrów w hydrolizatach biomasy po obróbce SAA. Ponadto, osiągnięto 38.7% oraz 68.9% poziom delignifikacji odpowiednio po procedurze SAA przeprowadzonej w 50 °C i 90 °C.
13
Content available remote The study of chemical composition and enzymatic hydrolysis efficiency of poplar wood (Populus deltoides x maximowiczii) after steam explosion pretreatment
88%
Antczak A. , Szadkowski J. , Marchwicka M. , Akus-Szylberg F. , Bytner O. , Zawadzki J.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
|
2018
|
tom No. 104
139--146
EN
A beneficial influence of the steam explosion pretreatment on simple sugars yield after enzymatic hydrolysis was observed. The highest average glucose content (25.8%) in the hydrolysate after steam explosion pretreatment at 190 oC was achieved. In turn, an application of steam explosion pretreatment at 205 oC resulted in a decrease of enzymatic hydrolysis efficiency and 21.8% of glucose was obtained only. In case of xylose, a similar correlations were observed. The highest average xylose content (4.0%) at 190 oC was obtained and the decrease of xylose content (to the level of 2.4%) after enzymatic hydrolysis of biomass pretreated at 205 oC was observed. Probably, the decrease of the sugars content was caused by a condensation reactions of lignin and polysaccharides. Other explanation of the obtained results may be formation of inhibitors, which could hinder enzymatic hydrolysis. This reason may be especially important, because in these studies to enzymatic hydrolysis process unwashed solid fraction was used. Obtained results were correlated to the chemical composition of the studied wood. The partial hemicelluloses degradation and dissolution led to decrease in its content and at the same time increased the content of extractives. Lignin content stayed roughly the same for both untreated material and steam explosion pretreated at 160 oC and 175 oC. However, its content increased rapidly after steam explosion pretreatment at 190 oC and 205 oC. Cellulose content was not a subject to significant changes, although its apparent increase, when compared to cellulose content in untreated wood, was probably due to decline in hemicelluloses content.
14
Content available Preparation of nanocellulose by hydrolysis with ionic liquids and two-step hydrolysis with ionic liquids and enzymes
88%
Babicka M. , Wozniak M. , Szentner K. , Borysiak S. , Dwiecki K. , Ratajczak I.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
|
2021
|
tom 116
15
Content available Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover
88%
Akus-Szylberg F. , Antczak A. , Zawadzki J.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
|
2021
|
tom 115
16
Content available remote Production of ethanol from wheat straw
88%
Smuga-Kogut M. , Wnuk A. D. , Zgórska K. , Kubiak M. S. , Wojdalski J. , Kupczyk A. , Szlachta J. , Luberański A.
Polish Journal of Chemical Technology
|
2015
|
tom 17
|
nr 3
89-94
EN
This study proposes a method for the production of ethanol from wheat straw lignocellulose where the raw material is chemically processed before hydrolysis and fermentation. The usefulness of wheat straw delignification was evaluated with the use of a 4:1 mixture of 95% ethanol and 65% HNO3 (V). Chemically processed lignocellulose was subjected to enzymatic hydrolysis to produce reducing sugars, which were converted to ethanol in the process of alcoholic fermentation. Chemical processing damages the molecular structure of wheat straw, thus improving ethanol yield. The removal of lignin from straw improves fermentation by eliminating lignin’s negative influence on the growth and viability of yeast cells. Straw pretreatment facilitates enzymatic hydrolysis by increasing the content of reducing sugars and ethanol per g in comparison with untreated wheat straw.
17
Intracellular polysaccharides in the mycelium of Aspergillus giganteus Wehm. mut. alba Zurz. I. TCA-soluble glucan
75%
Zurzycka A. , Pasiut H. , Nowak G.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1987
|
tom 35
|
nr 10-12
18
Content available remote Wpływ wstępnej obróbki słomy kukurydzianej gorącą wodą na jej skład chemiczny i hydrolizę enzymatyczną
75%
Akus-Szylberg F. , Antczak A. , Bytner O. , Radomski A. , Krajewski K. , Zawadzki J.
Przemysł Chemiczny
|
2018
|
tom T. 97, nr 11
1866--1869
PL
Przedstawiono wyniki badania składu chemicznego i hydrolizy enzymatycznej przeprowadzonej dla słomy kukurydzianej poddanej obróbce wstępnej metodą LHW w różnych temperaturach. Uzyskane wyniki pozwoliły stwierdzić, że wraz z rosnącą temperaturą procesu wzrastała zawartość otrzymywanej w hydrolizie enzymatycznej glukozy. Z kolei w przypadku ksylozy jej zawartość stopniowo malała. Stwierdzono również, że proces obróbki wstępnej metodą LHW miał korzystny wpływ na skład chemiczny słomy kukurydzianej jako potencjalnego substratu do produkcji bioetanolu.
EN
The shredded corn stover (chip size 0.43-1.0 mm) was treated with liq. hot H2O at 160-205°C and then treated with a com. enzyme in an aq. environment. The glucose and xylose contents in the hydrolyzates were detd. with high performance liq. chromatog. An increase of temp. in the thermal treatment resulted in an increase in glucose and decrease in xylose contents.
19
Kinetyka wielostopniowej hydrolizy enzymatycznej substratu białkowego
75%
Balejko J. A.
LAB Laboratoria, Aparatura, Badania
|
2004
|
tom R. 9, nr 6
13-14
20
Content available Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 50 °C
75%
Marchwicka M.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
|
2021
|
tom No. 114
52--59
EN
Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 50 °C. Cellulose obtained by the Kürschner-Hoffer method from the wood of 3-year-old poplar (Populus trichocarpa) was subjected to enzymatic hydrolysis. Cellic® CTec2 enzymes (Novozymes, Denmark) were used. The enzymatic hydrolysis was tested within the conditions recommended by the manufacturer and the literature. The process was carried out at 50 °C at various pH – 4.8, 5.0, 5.5 and enzymes doses - 25, 50 and 100 mg per 100 mg of the dry mass of cellulose. The process was ended after 24 h. The hydrolysates were analysed by high-performance liquid chromatography (HPLC) to determine the glucose content, and then the highest glucose yield. The highest glucose yield was obtained for pH 4.8 and 100 mg of enzymes per 100 mg of the dry mass of cellulose – 72 %.
PL
Wpływ pH i ilości enzymu Cellic® CTec2 na wydajnośc glukozy po hydrolizie enzymatycznej celulozy w 50 °C. Celulozę otrzymaną metodą Kürschnera-Hoffera z drewna 3-letniej topoli Populus trichocarpa poddano hydrolizie enzymatycznej. Zastosowano enzymy Cellic® CTec2 (Novozymes, Dania). Hydrolizę enzymatyczną badano w warunkach zalecanych przez producenta i literaturę. Proces prowadzono w temperaturze 50 °C przy różnym pH - 4,8, 5,0, 5,5 i dawkach enzymu - 25, 50 i 100 mg na 100 mg suchej masy celulozy. Proces został zakończony po 24 godzinach. Hydrolizaty analizowano metodą wysokosprawnej chromatografii cieczowej (HPLC) w celu określenia zawartości glukozy, a następnie największej wydajności glukozy. Największą wydajność uzyskano dla pH 4,8 i 100 mg enzymu na 100 mg suchej masy celulozy - 72%.
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