Wyniki wyszukiwania - Biblioteka Nauki

1

Effects of Insulin and Embryonic Stem Cells loaded PLGA Nanoparticles on Pancreatic Beta TC Cells

100%

Yücel Ç. , Aktaş Y. , Değim Z. , Yılmaz Ş. , Arsoy T. , Altıntaş L. , Çokçalışkan C. , Sözmen M.

Acta Poloniae Pharmaceutica - Drug Research

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2018

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tom 75

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nr 6

1377-1389

EN

In The present study, the aims was to investigate and compare the effect of insulin and embryonic stem cells (ESC) loaded nanoparticle formulations (NPs) on pancreatic-beta-TC-cell regeneration. Characterization studies of NPs were performed. Permeability of insulin and the effect of ESC on pancreatic beta cells were investigated. by the determination of Insulin or glucose levels were determined and histologic investigations were also performed. ESC encapsulation efficiency was calculated by western blot analysis. The particle sizes of insulin and ESC-loaded-NPs were determined as 0.665±0.202 µm and 0.650 ±0.310 µm. The mean zeta potentials of insulin and ESC-loaded-NPs nanoparticles were found as 6.88±0.729 mV, 5.13±0.631 mV. The polydispersity index of insulin and ESC nanoparticles were 0.660±0.175, 0.620±0.205 respectively. Encapsulation efficiency of insulin and ESC-loaded-NPs were found to be 50±1.53% and 51%. Insulin release from nanoparticles was found to be 72.8% over 48h. The gGlucose concentrations wasere decreased to 201 and 202.7 mg/dl from 250 mg/dl in streptozocin (STZ) induced diabetic mice group after insulin and ESC-loaded-NPs administration. Insulin and ESC-loaded-NPs improved the blood insulin levels in all experimental groups. These NPs may be used for repairing of pancreatic cells. Healing or some degree of regeneration was observed when insulin and ESC-loaded-NPs were administered to the mice ip. ESC-loaded-NPs can be a potential source for cell replacement therapy in the treatment of diabetes.

2

NKT cells: from totipotency to regenerative medicine

100%

Wakao H.

Archivum Immunologiae et Therapiae Experimentalis

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2009

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tom 57

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nr 2

117-128

3

Stem cells in nephrology: present status and future

86%

Watorek E. , Klinger M.

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tom 54

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nr 1

4

Myogenic stem cells

72%

Burdzinska A. , Gala K. , Paczek L.

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2008

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tom 46

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nr 4

401-412

5

Second-phase validation study of an alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies

72%

Lee J.-H. , Park S. Y. , Ahn C. , Yoo Y.-M. , Kim C.-W. , Kim J.-E. , Jo N. R. , Kang H. Y. , Jung E.-M. , Kim K.-S. , Choi K.-C. , Lee S. D. , Jeung E.-B.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 2

6

Techniques of human embryonic stem cell and induced pluripotent stem cell derivation

72%

Lewandowski J. , Kurpisz M.

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2016

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tom 64

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nr 5

7

Gene trapping: An antibody-dependent approach for verifying integration in your favorite gene

72%

Gorelik A. , Sapir T. , Reiner O.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 4

614-620

EN

Gene trapping is used to introduce genome-wide insertional mutations in embryonic stem cells. Determining the integration site is based on highthroughput PCR, which has inevitable possibilities for mistakes, thus necessitating clone verification prior to the generation of mutant mice. Here, we propose a rapid method to validate gene identity based on the fact that many high throughput gene-trapping integrations result in fusion proteins encompassing the N-terminal portion of the gene of interest and LacZ being expressed in embryonic stem cells. Our method utilizes an immunoprecipitation assay using a specific N-terminal-directed antibody to the protein product of the gene of interest followed by a color LacZ assay of the immunoprecipitate, strongly supporting the formation of a fusion protein when the color develops.

8

Inhibitory effect of octyl-phenol and bisphenol A on calcium signaling in cardiomyocyte differentiation of mouse embryonic stem cells

72%

Lee J.-H. , Yoo Y.-M. , Jung E.-M. , Ahn C. , Jeung E.-B.

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tom 70

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nr 3

9

Stem cells and their outstanding concerns

72%

Lukomska B.

Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii

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2012

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nr 1

EN

Stem cells have captured considerable scientific and clinic interest because of their potential to renew themselves and to differentiate into one or more adult cell types. Thus stem cells have been recognized as a potential tool for the development of innovative therapeutic strategies in different disease disorders. Stem cells can be discriminating based on their differentiated potential as totipotent, pluripotent, multipotent or unipotent cells. There are in general three types of stem cells: embryonic, fetal and adult stem cells. While embryonic stem cell therapy has a lot of ethical concerns due to their obtaining but also unlimited proliferation and uncontrolled differentiation, fetal and adult stem cells have been used in the treatment of different diseases. The bone marrow, peripheral blood and umbilical cord blood are ideal sources of adult stem cells because there are easily accessible and contain two types of stem cells: hematopoietic stem cells giving rise to all blood cell types and mesenchymal stem cells differentiating into cells of mesodermal lineage. This review describes the general characteristics of these stem cell populations and their current applications in regenerative medicine. Additionally induced pluripotent stem cells generated through the reprogramming of differentiated adult cells are described.

PL

Możliwość wspomagania regeneracji nieodwracalnie uszkodzonych tkanek i narządów za pomocą transplantacji komórek macierzystych skupia uwagę środowisk medycznych i opinii publicznej. Komórki macierzyste mają zdolność do samo-odnowy oraz do różnicowania się w dojrzałe komórki struktur całego organizmu. Na określonych etapach rozwoju osobniczego wykazują różny stopień ograniczenia potencjału do dalszego różnicowania, od komórek totipotencjalnych (zygota), poprzez pluripotencjalne (komórki zarodkowe) do multipotencjalnych lub unipotencjalnych (komórki somatyczne). Zarodkowe komórki macierzyste, z uwagi na nieograniczone zdolności namnażania i pluripotencjalność wydają się najbardziej optymalne do zastosowania w terapiach regeneracyjnych, lecz zastrzeżenia natury etyczno-moralnej pozyskiwania tych komórek, a także możliwość ich niekontrolowanej proliferacji po przeszczepieniu w organizmie biorcy skłoniły badaczy do poszukiwania alternatywnych źródeł komórek macierzystych, jakimi są tkanki dojrzałego organizmu. Badania ostatnich lat wykazały, iż w dojrzałych tkankach somatycznych znajdują się pluripotencjalne komórki macierzyste, co więcej odkrycia Takahashi i Yamanaka w roku 2006 r udokumentowały możliwość przekształcenia zróżnicowanych komórek izolowanych od osobników dorosłych w komórki pluripotencjalne. Zainicjowanie nowatorskich metod badawczych, których celem będzie opracowanie skutecznych sposobów przeszczepiania somatycznych komórek macierzystych w celach regeneracyjnych jest nadzieją w terapii wielu nieuleczalnych schorzeń.

10

Genetic manipulation of human embryonic stem cells

72%

McWhir J. , Didomenico A. , Hewitt Z. , Priddle H. , Thomson A.

Animal Science Papers and Reports. Supplement

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2004

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tom 22

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nr 1

11

Stem cells as therapy for cardiac disease — a review

58%

Jezierska-Wozniak K. , Mystkowska D. , Tutas A. , Jurkowski M. K.

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tom 49

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nr 1

12

Behavior, morphology and morphometry of equine adipose – derived mesenchymal stem cells in culture

58%

Marycz K. , Grzesiak J.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2011

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tom 14

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nr 2

13

Hybrid cells differentiate to hepatic lineage cells and repair oxidative damage

58%

Xu D. , Wang F. , Gu H. , Wang J. , Guo Q. , Zhang Y. , Wang Z.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 3

451-472

EN

Hybrid cells derived from stem cells play an important role in organogenesis, tissue regeneration and cancer formation. However, the fate of hybrid cells and their range of function are poorly understood. Fusing stem cells and somatic cells induces somatic cell reprogramming, and the resulting hybrid cells are embryonic stem cell-like cells. Therefore, we hypothesize that fusion-induced hybrid cells may behave like ES cells in certain microenvironments. In this study, human hepatic cells were induced to apoptosis with H2O2, and then co-cultured with hybrid cells that had been derived from mouse ES cells and human hepatic cells using a transwell. After co-culturing, the degree of apoptosis was evaluated using Annexin-V/PI double-staining analysis, flow cytometry and Western-blot. We observed that H2O2-induced cell apoptosis was inhibited by co-culture. In addition, the activity of injury-related enzymes (GSH-Px, LDH and SOD) and the level of albumin release in the co-culture system trended toward the level of normal undamaged hepatic cells. The stably increased levels of secretion of ALB in the co-culture system also confirmed that co-culture with hybrid cells helped in recovery from injury. The fate of the hybrid cells was studied by analyzing their gene expression and protein expression profiles. The results of RT-PCR indicated that during co-culturing, like ES cells, hybrid cells differentiated into hepatic lineage cells. Hybrid cells transcripted genes from both parental cell genomes. Via immunocytochemical analysis, hepatic directional differentiation of the hybrid cells was also confirmed. After injecting the hybrid cells into the mouse liver, the GFP-labeled transplanted cells were distributed in the hepatic lobules and engrafted into the liver structure. This research expands the knowledge of fusion-related events and the possible function of hybrid cells. Moreover, it could indicate a new route of differentiation from pluripotent cells to tissue-specific cells via conditional co-culture.

14

From embryonic stem cells and induced pluripotent cells to lymphocytes

51%

Seiler K. , Tornack J. , Karjalainen K. , Tsuneto M. , Melchers F.

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2011

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tom 59

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nr 5