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EN
The objective of the experiment was evaluation of some physical traits of egg quality in hens originating from Polish egg-type pedigree farms and destined for small-scale farming as well as in a breed of preserved chickens. 30 eggs from each of the three genetic groups (parental P55 Barred Rock strain, VH43 parental cross of V44 Rhode Island Red and H33 Leghorn strains, and the preserved Z11 Partridge Greenleg strain) were taken to analysis. The layers were maintained in the same environmental conditions and were under the same feeding regime.Analyses of egg quality were accomplished in 26th and 50th weeks of age, with an electronic EQM device. Egg weight in VH43 cross in the beginning of laying was 61.5 g and it was significantly bigger than in P55 hens (50.2 g) and Z11 hens (42 g). No statistical differences between experimental groups were found in shell thickness, shell density. Egg shell colour in P55 hens differed significantly from that in the other groups. The highest Haugh unit score (99.9) and albumen height (9.7 mm) were noted in the eggs of P55 hens in 26th weeks of age. These parameters lowered with age in all experimental groups. The highest proportion of yolk was found in the eggs of Z11 (29.5%) in 50 weeks of age. Yolk percentage increased with age in all genetic groups. Yolk colour index (La Roche) diminished with age of layers.
PL
Celem badań była ocena wybranych cech jakości jaj pochodzących od kur z polskich ferm zarodowych przeznaczonych do chowu drobnotowarowego oraz od rasy kur objętej programem ochrony zasobów genetycznych drobiu. Materiał do badań stanowiły jaja kur Barred Plymouth Rock (P55), mieszańców VH43 oraz jaja Zielononóżki kuropatwianej (Z-11). Masa jaja kur mieszańców VH43 na początku nieśności wynosiła 61,5 g i była istotnie większa niż masa jaj kur P55 (50,2 g) i kur Z-11 (42 g). Nie stwierdzono istotnych różnic w grubości i gęstości skorupy. Zanotowano statystycznie istotną różnicę w barwie skorupy jaj między kurami P55 a dwiema pozostałymi grupami. Największą wartość jednostek Haugha (99,9) oraz wysokości białka (9,7 mm) stwierdzono w jajach kur P55 w 26. tygodniu życia. Wartości te obniżały się wraz z przebiegiem nieśności w jajach wszystkich grup genetycznych. Największy procentowy udział żółtka stwierdzono w jajach kur Z-11 (29,5). Udział żółtka wzrastał wraz z wiekiem u wszystkich badanych grup, natomiast wskaźnik barwy żółtka (La Roche) zmniejszał się.
EN
Ionizing radiation (IR) is applied to inactivate the nuclear genome in rainbow trout eggs during induced androgenetic development. However, IR-generated reactive oxygen species (ROS) may affect developmental potential of eggs and reduce the effectiveness of androgenesis. To verify this assumption, androgenetic development of rainbow trout was induced in eggs irradiated with 350 Gy of X-rays. Survival rates, pH of the ovarian fluid and activity of antioxidant enzymes, including SOD, CAT and GPx, were examined in non-irradiated and irradiated eggs originating from four females. Survival rates of androgenetic embryos developing in eggs produced by different females varied from 1% to 57% and these inter-clutch differences were significant. Eggs from female F4, which showed the highest developmental competence for androgenesis, also showed increased activities of SOD, CAT and GPx enzymes. The pH value of the ovarian fluid of each female was over 8 before and after irradiation, therefore it seems that radiation did not affect the ovarian fluid pH. Considering the above-mentioned inter-clutch differences, a strong maternal effect on the effectiveness of androgenesis can be assumed. Eggs with increased activity of antioxidant enzymes before irradiation should be expected to show increased developmental competence for androgenesis.
EN
Background. Hatching is a process in which the embryo emerges from the egg by breaking the protective egg shell. Our preliminary observations indicate that hatching duration and time-distribution may vary among and within the fish species, and that some embryos fail to hatch or hatch incompletely, probably due to the “incorrect” hatching way. So the aim of this study was a detailed description of hatching of three fish species: common carp, barbel, and rainbow trout. Materials and methods. Three species of fish: common carp, Cyprinus carpio; barbel, Barbus barbus; and rainbow trout, Oncorhynchus mykiss; were examined for their hatching modes. The mode and the time of hatching of each larva were noted. Newly hatched larvae were counted and examined. The malformations were classified. Observations of embryos and larvae were done using the stereoscopic microscope Nikon connected to the computer with the MultiScan 8.4 image analysis system; the hatching embryos and larvae were photographed. Results. Three modes of hatching were observed, two of them similar in all three fish species. Some fish started hatching tail first from the egg shell, others head first or—specifically for barbel—yolk sac first. The data obtained in the present study showed that tail hatching was the most successful in all fish species, and shown by most good quality larvae. The majority of tail-hatched larvae developed normally and were viable, and only some of them were deformed and showed slight morphological defects, mainly single vertebral malformations that in most cases were negligible. Head hatching was the precarious in carp, and in all fish species less common and successful comparing to the tail hatching. Conclusion. The hatching mode could be used as another good parameter for estimation of quality of eggs and larvae.
EN
The examination was made in the experimental room where 36 laying hens were kept in battery system. The hens were divided into three groups (12 birds in each group) – one control group (C) and two experimental groups E-1 and E-2. The birds from the control group were fed with the all-mash prepared according to standard recipe; the content of calcium was 3.0%. The feed for the experimental groups contained also 3% of calcium but 0.5% (E-1) and 1.0% (E-2) of Ca was from CaO2 (51.8% of Ca, 16.7% of active oxygen, pH-value in 1% solution – 12.5). The experiment lasted 56 days. Total calcium concentration in blood serum samples and chosen qualitative parameters of eggs were analysed. It was found that eggshells thickness from laying hens of group E-2 was higher comparing with eggshells from control group and from group E-1 (p≤0.01). The eggshells from both experimental groups were more resistant (breaking strength) than eggshells from group C, especially the samples collected after 2 and 4 weeks of experiment (p≤0.01 and p≤0.05). The eggs from groups E-1 and E-2 had higher weight but that fact was not statistically confirmed. Calcium peroxide used in our experiment did not cause any discolouration of eggshells and had no influence on the colour of egg yolk. Calcium concentrations in blood serum were high (4.43 to 6.74 mmol/L) but not exceeded the values reported by other authors.
PL
Celem pracy było określenie wpływu nadtlenku wapnia (CaO2), jako źródła wapnia w paszy, na wyniki produkcyjne, wybrane parametry jakościowe jaj oraz zawartość wapnia całkowitego w surowicy krwi u niosek, jako kryterium jego bioprzyswajalności. Badania przeprowadzono w wiwarium na materiale 36 młodych kur nieśnych linii Lohmann Brown, utrzymywanych w systemie bateryjnym przez okres 8 tygodni. Stosowano standardowy program świetlny oraz zapewniono stały dostęp do paszy i wody. Ptaki podzielono na 3 grupy (po 12 niosek w grupie) – grupę kontrolną (K) oraz dwie grupy doświadczalne (D-1- i D-2). Ptaki otrzymywały pełnoporcjową mieszankę paszową typu J-297 o zawartości wapnia 3%. Z paszy dla grup D-1 i D-2 wycofano odpowiednio 0,5% i 1,0% czystego wapnia i uzupełniono poprzez dodatek wapnia z CaO2. Kontrolowano wskaźniki produkcyjne (nieśność, masa jaja, pobranie paszy), określono wybrane parametry jakościowe jaj. Jaja do badań pobrano w 4 seriach: w 14, 28, 42 i 56 dniu trwania doświadczenia. W surowicy krwi oznaczono poziom wapnia całkowitego oraz fosforu nieorganicznego. Stwierdzono wzrost grubości skorupy jaja od kur z grupy D-2 w stosunku do grup kontrolnej i D-1 (p≤0,01). W obu grupach doświadczalnych stwierdzono wzrost wytrzymałości skorup jaj w stosunku do jaj z grupy kontrolnej, szczególnie po 4 i 6 tygodniach podawania preparatu (p≤0,01 i p≤0,05). Jaja od kur otrzymujących nadtlenek wapnia charakteryzowały się wyższą masą, lecz nie zostało to potwierdzone statystycznie. Podawany nadtlenek wapnia nie powodował odbarwień żółtka jaja. Stężenie Ca i P w surowicy krwi kur było dość wysokie, lecz mieściło się w zakresie wartości fizjologicznych, co świadczy o prawidłowym zaopatrzeniu niosek w wapń.
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