Wyniki wyszukiwania - Biblioteka Nauki

1

Dojrzewanie in vitro oocytow kota domowego

100%

Grabiec A. , Faundez R. , Max A. , Tischner M.

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tom 63

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nr 03

318-321

EN

The aim of this study was to compare the efficiency of in vitro maturation in relationship to pathomorphology of uterus and oocytes quality. Morphological evaluation of the uterus helped to classify the queens into one of three groups: those with normal uteri, those with transformed uteri and pregnant queens. Cumulus-oocyte complexes were separated at recovery into three types according to pigmetation, uniformity and smoothness of ooplasm, compactness and number of layers of cumulus cells, as well as integrity of zona pellucida. Oocytes were maturated for 36 hours at 38°C in M199 medium containing cysteine and 17b oestradiol. After IVM, the meiotic status of oocytes was determined and the percentage of matured oocytes was calculated in relationship to COCs quality and uterus condition. In the group with normal uterus, oocytes of class A maturated (63.93%) better than oocytes class B and C, although the differences in maturation of oocytes class A and B were not statistically significant. The worst results of IVM were for oocytes class C. In the group of transformed uterus, 55.17% of oocytes class A maturated, while in only 7.84% of oocytes class C metaphase II had been observed. Significant differences were established in availability to IVM between oocytes class A and C as well as class B and C. In the group with pregnant uteri, the highest percentage of matured oocytes was in class A (46.27%), though there are no statistically significant differences in IVM between oocytes class A and B (28.8%), and the oocytes class C maturated in only 1.82%. Only oocytes of class A and B should be qualified for IVM procedure. Pregnant queens as well as queens with transformed uteri can also serve as oocyte donors for in vitro maturation.

2

The use of CBG-banding technique in chromosome studies of bovine oocytes matured in vivo

100%

Lechniak D. , Switonski M. , Sosnowski J. , Rusiecka E.

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nr 4

219-223

3

Rola cytokin z rodziny TGF-beta w procesie folikulogenezy, oogenezy oraz dojrzewania oocytów u ssaków

86%

Rybska M. , Kempisty B. , Swierczewska M. , Wozna M. , Ciesiolka S. , Piotrowska H. , Antosik P. , Bukowska D. , Jopek K. , Bruska M. , Nowicki M. , Jaskowski J. M.

Medycyna Weterynaryjna

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2015

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tom 71

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nr 07

EN

The process of oocytes maturation in mammals is regulated by the expression of many types of cell-specific genes. Both nuclear and cytoplasmic maturation is based on activation of biochemical and metabolic pathways that lead to reaching the full maturation stage (MII) of oocytes. The most recent data indicates that the proteins belonging to transforming the growth factor beta (TGF-β) superfamily play an important role in the process of oocytes maturation, as well as of reaching full developmental capacity by these cells. Moreover, it has been clearly showed that the proteins belonging to TGF-β such as inhibins (INHA, INHB) and activins regulate both folliculo- and oogenesis by the stimulation and/or inhibition of several biochemical pathways. In this review, the most recent knowledge about the role of proteins belonging to TGF-β superfamily in the regulation of folliculogenesis, oogenesis and oocytes maturation has been presented and discussed.

4

Wpływ dodatku leptyny na dojrzewanie oocytów bydła in vitro w warunkach stresu cieplnego

86%

Wozna M. , Jaskowski J. M. , Jackowska M. , Ludwiczak A. , Szczepanska P.

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2010

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tom 85

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nr 02

5

Proba oceny oocytow psa dla celow zaplodnienia in vitro

86%

Ptaszynska M. , Tischner M. , Gajewski Z. , Boryczko Z.

Nowa Weterynaria

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1996

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tom 01

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nr nr spec.[pazdziernik '96]

48-52

6

Potomstwo po przeniesieniu zarodkow wyprodukowanych z oocytow bydlecych uzyskanych metoda OPU

72%

Smorag Z. , Katska L. , Gogol P. , Jazdzewski J. , Dymarski I.

Przegląd Hodowlany

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1999

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tom 67

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nr 08

7-9

7

Uzyskiwanie zarodkow zwierzat gospodarskich in vitro

72%

Duszewska A. M. , Reklewski Z.

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tom 63

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nr 12

1522-1525

EN

The article presents the present state of knowledge about obtaining in vitro embryos from farm animals. This biotechnique includes: in vitro maturation of oocytes, in vitro fertilizing matured oocytes and in vitro culture of embryos. The aim of in vitro production of embryos is to obtain more blastocysts and blastocysts of good quality which will determine the efficiency of embryo transfer and facilitate the production of a greater number of healthy offspring. Offspring were produced after transferring embryos produced in vitro in sheep, cattle, pigs, goats and horses. This biotechnique is used in farm animal breeding, biotechnology and basic research.

8

Ocena wpływu soli hialuronianu sodu na dojrzałość mejotyczną i fragmentacje DNA oocytów bydlęcych dojrzewających in vitro - wstępne wyniki badań

72%

Latasiewicz E. , Opiela J.

Wiadomości Zootechniczne

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2011

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tom 49

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nr 4

9

Wybrane aspekty zwiazane z dojrzewaniem oocytow, zaplodnieniem oraz rozwojem zarodkowym u psow

72%

Bukowska D. , Kempisty B. , Antosik P. , Jaskowski J. M. , Olechnowicz J.

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nr 05

628-631

EN

The development of assisted reproductive techniques in dogs creates new possibilities to protect many species from extinction. The efficiency of oocytes maturation in bitches in vitro is much lower in comparison to other mammals. That is why there are many limitations in the development of methods of reproduction biotechnology in this species, i.e. embryo production (IVP), cryopreservation of semen or the transfer of nuclei. The oocytes of bitches existing in the follicle environment are influenced by the activity of progesterone, while the re-start and finish of the meiotic division in these cells takes place in the uterine tube. In contrast to bitches, in the case of the majority of mammals estrogens are dominating hormones and ovulating oocytes are in metaphase II of meiotic division. Differences in the process of oocytes maturation between several species of mammals make it impossible to create optimal and at the same time universal conditions of in vitro production. A relatively late implantation of the embryo in dogs can also be observed. In the present article questions connected with the process of in vivo and in vitro oocytes maturation were described as well as the fertilization and initial stages of embryo development in dogs.

10

Rola komórek pęcherzykowych w dojrzewaniu oocytów suki w warunkach in vitro

72%

Wlodarczyk R. , Bukowska D. , Jaskowski J. M.

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2010

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tom 85

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nr 01

11

Uzyskiwanie niedojrzalych oocytow zwierzat gospodarskich i ich dojrzewanie in vivo i in vitro w aspekcie wykorzystania potencjalu rozrodczego samicy

72%

Katska-Ksiazkiewicz L. , Opiela J.

Roczniki Naukowe Zootechniki. Monografie i Rozprawy

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2008

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tom 38

1-74

12

Molekularne aspekty procesu dojrzewania jądrowego i cytoplazmatycznego oocytów u świń

72%

Bukowska D. , Kempisty B. , Ciesiolka S. , Piotrowska H. , Antosik P. , Wozna M. , Porowski S. , Ociepa E. , Maryniak H. , Jaskowski J. M. , Bryja A. , Nowicki M.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 08

EN

The development of pig reproductive biotechnology has made it possible to use this species not only as farm animals but also as important models in biomedical research. Systems based on pig embryos from in vitro fertilization (IVF) are used much more frequently, although the quality of these embryos differs from those produced by in vivo fertilization. The most frequent problems in the IVF procedure are disturbances in both nuclear and cytoplasmic oocyte maturation, and the mechanism of polyspermy specific for this species. Although there has been progress in increasing the number of oocytes produced by in vitro maturation processes, the quality of embryos from these oocytes and their developmental competence is still unsatisfactory. It has been suggested that the most important factor in this process is the establishment of optimal conditions for the development and maturation of oocytes. In this review the authors attempt to explain the molecular basis of oocyte maturation, meiosis resumption, and meiotic spindle assembly. The article is also an opportunity to use the knowledge gained to date in order to increase the efficiency of animal breeding, to evaluate the genetic value of oocytes, as well as to improve assisted reproductive techniques (ART).

13

Białka regulujące proces dojrzewania oocytów u ssaków

72%

Swierczewska M. , Zaorska K. , Kempisty B. , Nowicki M.

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nr 11

EN

In this review, the molecular aspects of meiotic division and characteristics of proteins involved in mammalian oocytes maturation have been presented. Moreover, the role of competition has also been shown. The mammalian oocyte’s maturation is divided into: (i) nuclear maturation; (ii) cytoplasmic maturation; and (iii) genomic maturation. The maturation processes involves the stages associated with stored proteins and mRNA as well as the inherited changes in genomic DNA methylation. The proper procedure of all stages of maturation influences the achievement by oocytes of full developmental competence and fertilization ability. Both of these processes are associated with attaining by the embryos the ability of growth and development following embryonic genome activation (EGA). The stages of oocyte’s maturation are regulated by the expression of specific genes encoding proteins that are expressed during early folliculo-, and oogenesis, and is species dependent. The most important proteins regulating the process of oocytes maturation involves the following: transforming growth factor of the large super family group (TGF), connexins (Cx), mitogen activated protein kinase (MAPK), protein kinase A (PKA) and epidermal growth factor (EGF).

14

Czynniki wplywajace na dojrzewanie oocytow suki w warunkach in vivo oraz in vitro

72%

Wlodarczyk R. , Karon M. , Bukowska D. , Jaskowski J. M.

Życie Weterynaryjne

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2008

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tom 83

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nr 09

749-753

15

Potomstwo po transplantacji zarodkow kozich pochodzacych z dojrzewajacych i zapladnianych in vitro oocytow

72%

Katska L. , Kania G. , Rynska B. , Gajda B. , Smorag Z.

Medycyna Weterynaryjna

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2002

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tom 58

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nr 06

462-463

16

Pozyskiwanie zarodkow bydla w warunkach laboratoryjnych

72%

Cieslak D.

Bydło

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2006

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nr 08

40-42

17

Czynniki wplywajace na dojrzewanie oocytow psa w warunkach in vitro

72%

Wlodarczyk R. , Bukowska D. , Jaskowski J. M.

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tom 63

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nr 04

389-393

EN

Modern methods of supported reproduction find little application in relation to dogs. Although there is some progress in the production dogs embryos in vitro, results to date remain far from unsatisfactory. The main cause of failures is the low ability of dog oocytes to end the maturation process. The percentage of dog oocytes matured in vitro does not exceed 24% while in different animals species this percentage was, respectively, in goats, cattle and pigs 94%, 74.5% and 58%. There are a good deal of studies to make optimal composition of a maturation medium. Oocyte incubation in a medium with the addition of hCG resulted in a maturation of 31.7% oocytes, while in a medium without hCG this percentage was 23.3%. Estrogens are important in the process of meiosis resumption and oocyte maturation. The addition of these hormones to the medium caused a significant increase of the percentage of maturated oocytes from 2.9% to 14.7% respectively in control and experimental groups. In a few studies the influence of such supplements as synthetic oviductal fluid (SOF) and the epithelial cells of oviduct on maturation dogs oocytes was definite. It seems that these supplements had no significant influence on initiation of meiosis resumption connected with oocyte maturation. However a positive influence on the enlargement of the percentage of matured dog oocytes was evoked by a culture condensation and an addition of cummulus cells.

18

Przedowulacyjne stadia dojrzałości oocytów jako narzędzie służące do synchronizacji owulacji w kontrolowanym rozrodzie okonia

72%

Krejszeff S. , Palinska-Zarska K. , Zarski D.

Komunikaty Rybackie

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2018

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nr 4

19

Ocena kompetencji rozwojowej oocytów oraz zarodków świń na podstawie badań molekularnych i mikrofluidycznych

58%

Kempisty B. , Walczak R. , Sniadek P. , Dziuban J. , Piotrowska H. , Zawierucha P. , Zaorska K. , Antosik P. , Bukowska D. , Jaskowski J. M. , Nowicki M. , Zabel M.

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2011

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tom 67

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nr 10

EN

Evaluation of oocyte developmental competence has an important influence on the ability of these cells to attain maturation, successful fertilization, development of embryo to the blastocyst stage and proper implantation. Factors determining the reproductive potential of gametes included: (1) expression of important transcription factors, (2) epigenetic changes, which influence the silencing of selected genes transcription, (3) transcription regulation, and (4) post-transcriptional regulation. The epigenetic changes mainly include: DNA methylation, histones modifications and changes in chromatin structure in the oocytes. In several studies, the association between oocyte morphology (mostly determined by cumulus cell layers and granularity of cytoplasm) and the ability of these cells to attain maturation and fertilization has been described. The use of biochemical, metabolomical and molecular markers is the most frequently applied tool in the assessment of oocyte developmental competence. However, most of these methods are invasive and lead to the decreased viability of the analyzed cells. Searching for new, objective and noninvasive techniques leads to the development of a microfluidic chip system, which shows the physical (spectral) properties of oocytes and embryos in comparison to the biological parameters. In this article, selected issues associated with the genetic regulation of such processes as: maturation of oocytes, fertilization and early stages of embryonic development, have been presented. Moreover, the regulation of transcription and post-transcriptional modification during oogenesis and embryogenesis in mammals, with special relation to pigs and the possibilities of applying of microfluidics in assessment of oocyte and embryo developmental competence was shown.

20

Biotechniki w rozrodzie kotowatych stan wiedzy, perspektywy i wyzwania

58%

Nizanski W. , Mikolajewska N. , Partyka A. , Ochota M.

Medycyna Weterynaryjna

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2012

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tom 68

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nr 09

EN

Owing to a drastic decline in the population of wild cats and a growing interest among cat breeders, the development of assisted reproductive techniques for felines has been an important subject of research over the last decade. In this paper we describe the present state of knowledge and prospects for development in this field. The paper is divided into sections dealing with the collection and preservation of semen, artificial insemination, oocyte collection, in vitro fertilization and cryopreservation of eggs. The paper also discusses the apoptotic events occurring in gametes, which are crucial for the development of assisted reproductive techniques.