Wyniki wyszukiwania - Biblioteka Nauki

1

Dojrzewanie in vitro oocytow kota domowego

100%

Grabiec A. , Faundez R. , Max A. , Tischner M.

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tom 63

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nr 03

318-321

EN

The aim of this study was to compare the efficiency of in vitro maturation in relationship to pathomorphology of uterus and oocytes quality. Morphological evaluation of the uterus helped to classify the queens into one of three groups: those with normal uteri, those with transformed uteri and pregnant queens. Cumulus-oocyte complexes were separated at recovery into three types according to pigmetation, uniformity and smoothness of ooplasm, compactness and number of layers of cumulus cells, as well as integrity of zona pellucida. Oocytes were maturated for 36 hours at 38°C in M199 medium containing cysteine and 17b oestradiol. After IVM, the meiotic status of oocytes was determined and the percentage of matured oocytes was calculated in relationship to COCs quality and uterus condition. In the group with normal uterus, oocytes of class A maturated (63.93%) better than oocytes class B and C, although the differences in maturation of oocytes class A and B were not statistically significant. The worst results of IVM were for oocytes class C. In the group of transformed uterus, 55.17% of oocytes class A maturated, while in only 7.84% of oocytes class C metaphase II had been observed. Significant differences were established in availability to IVM between oocytes class A and C as well as class B and C. In the group with pregnant uteri, the highest percentage of matured oocytes was in class A (46.27%), though there are no statistically significant differences in IVM between oocytes class A and B (28.8%), and the oocytes class C maturated in only 1.82%. Only oocytes of class A and B should be qualified for IVM procedure. Pregnant queens as well as queens with transformed uteri can also serve as oocyte donors for in vitro maturation.

2

Wpływ dodatku leptyny na dojrzewanie oocytów bydła in vitro w warunkach stresu cieplnego

86%

Wozna M. , Jaskowski J. M. , Jackowska M. , Ludwiczak A. , Szczepanska P.

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2010

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tom 85

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nr 02

3

Pozyskiwanie i rozwój in vitro oocytów kota domowego w świetle współczesnych badań

86%

Ochota M. , Nizanski W. , Twardon J.

Medycyna Weterynaryjna

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2010

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tom 66

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nr 10

s.659-662,bibliogr.

EN

Currently ART (assisted reproductive technology) is assuming one of the most important places in animal reproduction. Till the present, the main interest concerned the oocytes and embryos in laboratory and in producing animals. Nowadays, with the improvement of ART, as well as gradually increasing interest in endangered species, the interest in using these techniques is shifting toward other animals. Because of this fact, ART procedures concerning domestic cats have progressed significantly in the last years. Domestic cats are a very suitable model for the investigation of endangered feline species. The following paper discusses the current procedures of obtaining oocytes from domestic cats as well as their in vitro development.

4

Zdolność oocytów klaczy do dojrzewania i zapłodnienia in vitro

72%

Mlodawska W.

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tom 70

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nr 01

EN

The aim of the review was to present current views on the factors influencing the in vitro maturation (IVM) and fertilization (IVF) of mare oocytes. The first two foals produced with the use of the so-called standard IVF (co-incubation of oocytes and spermatozoa in culture media) were born over 20 years ago. To date, it has been possible to obtain offspring in horses after the fertilization of in vitro matured oocytes by the intracytoplasmic sperm injection technique (ICSI) or by the surgical transfer of oocytes to the oviducts of inseminated mares (fertilization in vivo). Causes of the low efficiency of IVF in horses are complex and may be related to an incomplete maturation of oocytes, an inappropriate method of sperm capacitation in vitro, as well as the use of non-compliant media for the development of inseminated oocytes and/or early embryos. The paper describes the method of oocyte collection from mare ovaries and the most important factors influencing the number and quality of oocytes obtained. It discusses the relationship between the physiological status of ovarian follicles, cumulus oophorus morphology and the capacity of mare oocytes for in vitro maturation and fertilization. In addition, some aspects of nuclear and cytoplasmic maturation of equine oocytes are presented. The understanding of the in vivo maturation mechanisms of equine gametes and of the developmental requirements of embryos helps to improve culture conditions and the in vitro fertilization efficiency of equine oocytes.

5

Badania nad pozaustrojową produkcją zarodków

72%

Duszewska A. , Reklewski Z.

Zeszyty Naukowe. Przegląd Hodowlany

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2000

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tom 54

6

Wpływ octanu kortyzonu i homogenatu przysadki mózgowej karpia na oocyty karpia w ostatnim etapie dojrzewania (badania in vitro)

72%

Popek W. , Blacha P. , Sionkowski J. , Szymacha J. , Epler P.

Roczniki Naukowe Zootechniki. Suplement

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2005

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tom 22

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nr 2

7

Uzyskiwanie zarodkow zwierzat gospodarskich in vitro

72%

Duszewska A. M. , Reklewski Z.

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tom 63

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nr 12

1522-1525

EN

The article presents the present state of knowledge about obtaining in vitro embryos from farm animals. This biotechnique includes: in vitro maturation of oocytes, in vitro fertilizing matured oocytes and in vitro culture of embryos. The aim of in vitro production of embryos is to obtain more blastocysts and blastocysts of good quality which will determine the efficiency of embryo transfer and facilitate the production of a greater number of healthy offspring. Offspring were produced after transferring embryos produced in vitro in sheep, cattle, pigs, goats and horses. This biotechnique is used in farm animal breeding, biotechnology and basic research.

8

Ocena wpływu soli hialuronianu sodu na dojrzałość mejotyczną i fragmentacje DNA oocytów bydlęcych dojrzewających in vitro - wstępne wyniki badań

72%

Latasiewicz E. , Opiela J.

Wiadomości Zootechniczne

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2011

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tom 49

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nr 4

9

Wybrane aspekty zwiazane z dojrzewaniem oocytow, zaplodnieniem oraz rozwojem zarodkowym u psow

72%

Bukowska D. , Kempisty B. , Antosik P. , Jaskowski J. M. , Olechnowicz J.

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nr 05

628-631

EN

The development of assisted reproductive techniques in dogs creates new possibilities to protect many species from extinction. The efficiency of oocytes maturation in bitches in vitro is much lower in comparison to other mammals. That is why there are many limitations in the development of methods of reproduction biotechnology in this species, i.e. embryo production (IVP), cryopreservation of semen or the transfer of nuclei. The oocytes of bitches existing in the follicle environment are influenced by the activity of progesterone, while the re-start and finish of the meiotic division in these cells takes place in the uterine tube. In contrast to bitches, in the case of the majority of mammals estrogens are dominating hormones and ovulating oocytes are in metaphase II of meiotic division. Differences in the process of oocytes maturation between several species of mammals make it impossible to create optimal and at the same time universal conditions of in vitro production. A relatively late implantation of the embryo in dogs can also be observed. In the present article questions connected with the process of in vivo and in vitro oocytes maturation were described as well as the fertilization and initial stages of embryo development in dogs.

10

Rola komórek pęcherzykowych w dojrzewaniu oocytów suki w warunkach in vitro

72%

Wlodarczyk R. , Bukowska D. , Jaskowski J. M.

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2010

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tom 85

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nr 01

11

Dobowe zmiany w oddziaływaniu hormonów na dojrzewanie oocytów karpia, in vitro

72%

Popek W. , Luszczek-Trojnar E. , Drag-Kozak E. , Epler P.

Roczniki Naukowe Zootechniki. Suplement

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2003

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tom 17

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nr 2

12

Uzyskiwanie niedojrzalych oocytow zwierzat gospodarskich i ich dojrzewanie in vivo i in vitro w aspekcie wykorzystania potencjalu rozrodczego samicy

72%

Katska-Ksiazkiewicz L. , Opiela J.

Roczniki Naukowe Zootechniki. Monografie i Rozprawy

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2008

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tom 38

1-74

13

Molekularne aspekty procesu dojrzewania jądrowego i cytoplazmatycznego oocytów u świń

72%

Bukowska D. , Kempisty B. , Ciesiolka S. , Piotrowska H. , Antosik P. , Wozna M. , Porowski S. , Ociepa E. , Maryniak H. , Jaskowski J. M. , Bryja A. , Nowicki M.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 08

EN

The development of pig reproductive biotechnology has made it possible to use this species not only as farm animals but also as important models in biomedical research. Systems based on pig embryos from in vitro fertilization (IVF) are used much more frequently, although the quality of these embryos differs from those produced by in vivo fertilization. The most frequent problems in the IVF procedure are disturbances in both nuclear and cytoplasmic oocyte maturation, and the mechanism of polyspermy specific for this species. Although there has been progress in increasing the number of oocytes produced by in vitro maturation processes, the quality of embryos from these oocytes and their developmental competence is still unsatisfactory. It has been suggested that the most important factor in this process is the establishment of optimal conditions for the development and maturation of oocytes. In this review the authors attempt to explain the molecular basis of oocyte maturation, meiosis resumption, and meiotic spindle assembly. The article is also an opportunity to use the knowledge gained to date in order to increase the efficiency of animal breeding, to evaluate the genetic value of oocytes, as well as to improve assisted reproductive techniques (ART).

14

Pozaustrojowe dojrzewanie i zapłodnienie oocytów zwierząt gospodarskich oraz hodowla in vitro zarodków

72%

Katska L. , Rynska B. , Smorag Z.

Roczniki Naukowe Zootechniki. Suplement

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1997

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tom 1

15

Czynniki wplywajace na dojrzewanie oocytow suki w warunkach in vivo oraz in vitro

72%

Wlodarczyk R. , Karon M. , Bukowska D. , Jaskowski J. M.

Życie Weterynaryjne

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2008

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tom 83

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nr 09

749-753

16

Developmental competence of bovine IVM/IVF oocytes under different co-culture conditions

72%

Katska L. , Rynska B. , Smorag Z.

Journal of Animal and Feed Sciences

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1998

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tom 07

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nr 3

353-362

17

Pozyskiwanie zarodkow bydla w warunkach laboratoryjnych

72%

Cieslak D.

Bydło

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2006

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nr 08

40-42

18

Czynniki wplywajace na dojrzewanie oocytow psa w warunkach in vitro

72%

Wlodarczyk R. , Bukowska D. , Jaskowski J. M.

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tom 63

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nr 04

389-393

EN

Modern methods of supported reproduction find little application in relation to dogs. Although there is some progress in the production dogs embryos in vitro, results to date remain far from unsatisfactory. The main cause of failures is the low ability of dog oocytes to end the maturation process. The percentage of dog oocytes matured in vitro does not exceed 24% while in different animals species this percentage was, respectively, in goats, cattle and pigs 94%, 74.5% and 58%. There are a good deal of studies to make optimal composition of a maturation medium. Oocyte incubation in a medium with the addition of hCG resulted in a maturation of 31.7% oocytes, while in a medium without hCG this percentage was 23.3%. Estrogens are important in the process of meiosis resumption and oocyte maturation. The addition of these hormones to the medium caused a significant increase of the percentage of maturated oocytes from 2.9% to 14.7% respectively in control and experimental groups. In a few studies the influence of such supplements as synthetic oviductal fluid (SOF) and the epithelial cells of oviduct on maturation dogs oocytes was definite. It seems that these supplements had no significant influence on initiation of meiosis resumption connected with oocyte maturation. However a positive influence on the enlargement of the percentage of matured dog oocytes was evoked by a culture condensation and an addition of cummulus cells.

19

Wpływ komórek ziarnistych i pożywek hodowlanych na kompetencje mejotyczne oocytów bydła oraz ich zdolność do rozwoju po zapłodnieniu in vitro

72%

Mlodawska W. , Ploszaj E.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 05

EN

The aim of the study was to compare the effect of gonadotropic hormones and granulosa cells on the maturation and developmental capacity of cattle oocytes in vitro, as well as the effect of TCM 199 and DMEM/F12 media on the development of embryos obtained in co-culture with oviduct epithelial cells. Fertilization was performed with the use of frozen semen from 2 bulls. Twenty hours after insemination, presumptive zygotes were placed in co-culture with oviduct cells in a TCM 199 (TCM-KJ co-culture) or a DMEM/F12 medium (DMEM-KJ co-culture) and cultured for 7-9 days. Metaphase II was reached by 40% and 48% of oocytes cultured in the presence of granulosa cells and gonadotropins, respectively. Only embryos obtained from oocytes maturing in the presence of granulosa cells developed to the blastocyst stage. Considerably more dividing embryos were obtained when the presumptive zygotes were co-cultured with TCM-KJ (38.1%) rather than with DMEM-KJ (8.6%; P < 0.01). This study showed that the presence of granulosa cells had no effect on the nuclear maturation of cattle oocytes, but increased their capacity for embryonic development. TCM 199 is much more useful than DMEM/F12 for the co-culture of cattle embryos with oviduct cells.

20

Analiza cytogenetyczna gamet i zarodków produkowanych in vitro

72%

Sosnowski J. , Waroczyk M. , Pawlak M. , Lechniak D. , Switonski M.

Zeszyty Naukowe. Przegląd Hodowlany

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1999

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tom 40