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3
84%
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tom 96
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nr 04
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2010
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tom 66
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nr 10
s.706-710,tab.,bibliogr.
EN
The aim of this work was to estimate the influence of packaging forms and freezing methods of the semen of boars on the reproductive performance of sows. Experiments were carried out with 50 sows in a large swine farm. The females in experimental groups were inseminated with FT whilst in the control groups with liquid semen. Ejaculates from 4 boars were frozen in a polystyrene box onto static LN nitrogen vapor in 5 ml maxi and 0.5 ml medium straws according to methods A and B, modifications of the Westendorf et al (1975) and of the Pursel and Park (1987) technique, respectively. The sows were divided into five research groups, ten pigs in each group. The sows in experimental groups 1, 2, 3, and 4 were inseminated with frozen semen whilst in control group 5 with liquid semen. The females in groups 1 and 2 were inseminated with the semen frozen using method A and confectioned in a 0.5 ml medium and in 5 ml maxi straws, respectively. Whilst the sows in the group 3 and 4 were inseminated with the semen frozen according to method B and packaged in 0.5 ml and 5 ml straws, respectively, in each group. One insemination dose consisted of either 5 billion spermatozoa placed into one 5 ml maxi straw or of ten 0.5 ml medium straws with 500 million spermatozoa. The sows were inseminated twice or three times every ten hours with frozen semen. The first insemination took place 24 h after the sow had first shown a standing reflex. Females inseminated with frozen semen were inseminated post cervically, three times every 10 h during each oestrus. One insemination dose comprises 5 × 10⁹ of sperm and 5mg of PGF₂α. The occurrence of ovulation was detected by ultrasonography. Efficiency of insemination and reproductive performance of sows have been evaluated on the basis of conception and farrowing rates and total piglets born in a litter. The conception rate, the farrowing rate and the total piglets born in a litter in all five groups (1, 2, 3, 4, 5) were: 100, 90, 100, 90, 100%; 80, 70, 90, 70, 100% and 10.62 ± 1.92; 9.42 ± 1.51; 10.77 ± 2.53; 9.71 ± 1.79; 12.0 ± 1.8, respectively. A total of 40 females were inseminated with frozen (experimental) and 10 with liquid semen. There were no statistically significant differences in pregnancy and farrowing rates between all the experimental groups. Sows inseminated with liquid semen gave significantly higher percentages of litter size than females inseminated with frozen semen (12.00 vs. 10.19) (Table 1). Sows in groups 1 and 3 inseminated with semen frozen in 0.5 ml straws according to methods A and B had significantly higher litter sizes than sows in groups 2 and 4 inseminated with semen frozen in 5 ml straws according to corresponding methods A and B (10.62 and 10.77 vs. 9.42 and 9.71), respectively (Table 2). Animals inseminated with semen frozen in 0.5 ml straws had significantly higher percentages of litter size than females inseminated with semen frozen in 5 ml straws (10.7 vs. 9.57) (Table 3). There were no significant differences in litter size between females inseminated with semen frozen according to methods A and B (10.06 vs. 10.31) (Table 4). Acceptable reproductive performance of female pigs after AI with frozen semen was probably achieved because special attention was paid to the heat detection and timing of insemination related to ovulation (with the aid of rectal and abdominal ultrasonography), post-cervical insemination, addition of PGF₂α to each insemination dose, improvement of the freezing-thawing methods and good freezability of the sperms’ donors. Both freezing methods are relatively simple, but method B is less time consuming in preparation than method A. Fertility results obtained with frozen-thawed boar semen in our experiments are quite satisfactory. These results indicate that under good conditions (insemination strategy) frozen boar semen can give results that approach those obtained with fresh semen.
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tom 54
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nr 02
123-125
EN
The aim of the study was to improve the methods of recovery, maturation and fertilization in vitro (IVМ/IVF) of lamb oocytes in order to get offspring from prepubertal donors. Oocytes were obtained from 4-9-week-old lambs, oocytes from slaughtered adult ewes were run in parallel as control. Ewe lambs received subcutaneous implants (1 mg norgestomet) for 5 days. At the time of implant removal the animals were injected with FSH and PMSG. After 24 h oocytes (60 per donor) were collected by laparotomy under general anesthesia. Oocytes were matured and fertilized in vitro 24 hours after IVЕ, presumptive zygotes were transferred to the intermediate recipients for 6 days. From 9 definitive recipients which received 18 blastocysts, 8 were confirmed pregnant by scanning performed on days 35 and 60. One sheep has already delivered two normal lambs.
EN
Experiments concerning the possibility of using large quantities of lucerne meal instead of barley for farrow and feeding sows throughout three breeding cycles. Gilts were divided at random into three groups of 10 and fed individually. Sows from the first group were given a fill containing 10% of lucerne meal instead of barley, the second group was fed 30% and the third group 50% of this component. There was no compensation of the dosage as regards energy and general protein. Fodder was given once daily in the morning. Up to the 90th day of pregnancy the sows received daily 2.4 kg of mixture in the first and second group and 2.5 kg in the third group. The daily ration was increased from the 91st to the 113th day of pregnancy to 3.4 kg in the first and second group and to 3.5 kg in the third group. During lactation all sows were fed at will and during barrenness - as they were during the first half of pregnancy. The piglets were weaned in the 42 day of life, observations were continued up to the 56th day. Attained results indicate that mixtures II and III containing 30 and 50% of lucerne meal included insufficient energy to be used in practice in feeding sows during the entire reproduction period.
EN
In 1980-1984 we analysed the reproduction indices in 160 mares of Wielkopolska Breed culled in the stud in Pępowo. The results were analysed statistically. The calculated indices were as follows: the time of reproduction performance of mares was 9.3 years /2-19/, pregnancy index - 77.9% including 72.3% deliveries and 5-6% abortions, the length of pregnancy - 325.87 days /280-357 days/, pregnancies giving stallions - 327.57 days, mares - 324,77 days /signifioant difference/. The length of pregnancy was affected by: offspring sex, the time of cowering, the number of pregnancies and a breeding horse. Thoroughbred stallions and one Angloarab caused longer pregnancies than those by half- bred stallions. Foaling took place from Sept. till May being intensified in Jan., Feb., March /51.88%/. The number of alive foals averaged 6.18. The effectiveness of having a female covered was affected by the month of covering, the age of a mare, a breeding horse and termination of previous pregnancy. An average interpregnancy period was 122,2 days /7-1168 days/.
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