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Content available remote Overproduction and purification of the CcpA protein from Lactococcus lactis.
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In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6×His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 Μg/ml ampicillin at 37°C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.
EN
Intermodular interactions play an important role in many processes at the molecular level. In the contemporary science, there is a growing interest concerning the characteristics of such interactions. Therefore, the computational chemistry can provide answers to many questions, which could not be answered using experimental methods. The Symmetry-Adapted Perturbation Theory (SAPT) method was applied to characterize the energy partitioning in dimers, trimers and microsolvation models. The investigated complexes belong to various classes of compounds, e.g. • dimers of: NH3 ˑˑˑHX, HF-pyridine, cycloalkanes, hypohalous acids; • trimers of: NH3 ˑˑˑNH3ˑˑˑHF or NH3ˑˑˑHFˑˑˑHF; • microsolvation models (biotin - water molecules). The current study summarizes recent years of our research devoted to the intermolecular interactions.
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