Currently, the evaluation of scientific journals is one of the most important tools in scientific policy, and points awarded for publications in journals play a key role in assessing the achievements of researchers and in the parametric evaluation of scientific institutions. The aim of the study was to examine the contribution of Polish journals from the field of life sciences with Impact Factor in the international bibliometric databases (Web of Science™, Scopus, Google Scholar, Index Copernicus) as well as in the lists of the Ministry of Science and Higher Education in Poland. The bibliometric analysis dealt with seven journals: Acta Biologica Cracoviensia Series Botanica, Archives of Environmental Protection, Dendrobiology, Acta Theriologica (currently Mammal Research), Polish Journal of Ecology, Acta Societatis Botanicorum Poloniae and Sylwan. The bibliometric techniques most often used in specialized databases were analysed based on the values of following indicators: Impact Factor, Hirsch Index, Egghe Index, SCImago Journal Rank and Index Copernicus Value. Acta Theriologica (category ‘zoology’), which was the most cited title with the highest value of the Impact Factor, was the most influential periodic in Polish science in 2000−2015 period among the analysed journals. In case of Dendrobiology and Sylwan, i.e. journals belonging to the ‘forestry’ category, the previous was listed higher in the analysed databases. However, the continuous increase in the value of Impact Factor and, as a consequence the development of the journal prestige, are observed for Sylwan.