Wyniki wyszukiwania - Biblioteka Nauki

1

Cytological features of various microspore derivatives appearing during culture of isolated maize microspores

100%

Goralski G. , Rozier F. , Matthys-Rochon E. , Przywara L.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

|

nr 1

EN

Microspore derivatives occurring in culture of maize microspores were studied by light and electron microscopy. The cultures showed a high degree of heterogeneity throughout the whole period of cultivation. Several types of androgenic structures at different developmental stages were observed, indicating a high level of asynchrony among microspores following the androgenic pathway. During the first 5 to 7 days of culture, multicellular structures developed inside the exine. At later stages of culture the developing microspores ruptured the pollen wall and formed structures variable in cellular characteristics. Three main types were distinguished: (i) one-domain structures consisting of small or large cells only; (ii) two-domain structures with large and small cells, the domains differing in size and cellular characteristics; (iii) callus-like structures. The two-domain structures seem to resemble zygotic proembryos which also contain two different parts, the suspensor and the apical region. The observed variability is most likely related to differences in gene activity during the inductive stage of androgenesis and the genetic properties of the microspores themselves, rather than to the conditions of in vitro culture.

2

Paramecium jenningsi species complex (Ciliophora, Protista) in India: the first description of new stands of P. bijenningsi and P. trijenningsi

84%

Przybos E. , Kalavati C. , Fokin S. I. , Tarcz S.

Folia Biologica

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2017

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tom 65

|

nr 1

3

Histological and cytological analysis of microsporogenesis and microgametogenesis of the invasive species Galinsoga quadriradiata Ruiz and Pav. (Asteraceae)

84%

Kolczyk J. , Tuleja M. , Plachno B. J.

Acta Biologica Cracoviensia. Series Botanica

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2015

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tom 57

|

nr 2

4

In vitro culture of unpollinated ovules of Viola odorata L.

84%

Stepien M. , Kuta E. , Przywara L.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

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1997

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tom 39

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nr 1

5

Chromosomes of Lupinus hispanicus subsp. hispanicus Boiss. et Reut., L.luteus L. and their hybrids

84%

Naganowska B. , Ladon D.

Journal of Applied Genetics

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2000

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tom 41

|

nr 3

6

Cytological and genetic evaluation of anther culture-derived doubled haploids in barley

84%

Szarejko I. , Falk D. E. , Janusz A. , Nabialkowska D.

Journal of Applied Genetics

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1997

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tom 38

|

nr 4

EN

Gametoclonal variation among anther culture-derived plants of three barley genotypes was estimated on the basis of cytological analysis (DH1, DH2 generation), observation of morphological variants (DH2, DH3) and chlorophyll mutation test (DH2, DH3). Individual head rows were grown in the field to detect possible chimeric structure of regenerants and to assess the number of variants and mutations in each line. Spontaneously doubled plants were the most frequent class (70%) among regenerants and almost 90% of them were completely fertile. There was a difference in proportion of haploids produced by different genotypes, but the highest frequency observed did not exceed 21%. The remaining regenerants were tetraploid, and contained chromosomal mutations or chimeras. In total, there were about 15% of polyploids and plants carrying chromosomal aberrations (translocations, inversions) among DH1 individuals. The changes in chromosome number and structure were the main source of observed variation. The level of gene mutation induced in vitro was relatively low. No more than 1% of microspore-derived plants expressed visible morphological changes in DH2 progeny. Only two morphological variants derived from the Bruce cultivar proved to be homozygous mutants (dwarf type) stable up the to third generation. The frequency of DH plants carrying chlorophyll mutation was 5.8%, but most of them (82%) were chimeric and had only a small mutation sector. The level of gametoclonal variation depended on the donor plant genotype. The highest proportion of variants and mutations was observed among DH plants derived from the Bruce cultivar, while the lowest was recorded among plants regenerated from anther culture of the doubled haploid line H930-36. Mechanisms leading to the observed variation and implications resulting from the presented experiments concerning implementation of anther culture in barley breeding were discussed. It was concluded that this method resulted in a high frequency of spontaneous doubling, a low frequency of genetic changes, and being less time and effort-consuming than the ’Bulbosum’ technique, can be applied to most barley breeding programs.

7

Karyological studies on Aconitum lasiocarpum [Rchb.] Gayer [Ranunculaceae]

84%

Joachimiak A. , Ilnicki T. , Mitka J.

Acta Biologica Cracoviensia. Series Botanica

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1999

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tom 41

EN

The karyotype structure of Aconitum lasiocarpum (Rchb.) Gáyer was investigated conventionally and by Giemsa C-banding. The chromosome complex of A. lasiocarpum is composed of two distinguishable pairs of long chromosomes and six pairs of considerably shorter chromosomes. Nucleolar organizers are localized on the shorter arms of three (1, 3 and 5) chromosomes. In all analyzed plants the first and third NOR-chromosome pairs show structural heterozygosity concerning the presence of small satellites. The C-banded karyotype of A. lasiocarpum is heterochromatin-poor; all fixed heterochromatin segments occupy 7.51% of karyotype length. Four satellited chromosomes have larger, terminally located heterochromatic segments on their shorter arms. The authors made preliminary karyological observations of two other Aconitum species, the closely related A. degenii and A. variegatum, and discussed the relationships between the three species.

8

Adventitious shoot regeneration from leaf explants of Malus domestica Borkh. in vitro

67%

Podwyszynska M. , Sowik I.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

|

nr 1

9

Callus induction and gametophyte regeneration in moss cultures

67%

Sokal I. , Kuta E. , Przywara L.

Acta Biologica Cracoviensia. Series Botanica

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1997

|

tom 39

EN

Callus induction was achieved from the protonema, whole gametophyte and gametophyte shoot cells in Atrichum undulatum, Bryum caespiticium and Polytrichum commune on modified MS medium containing 4% (w/v) glucose as the carbon source, MS + 4% (w/v) glucose + 1 mg/l 2,4-D + 1 mg/l kinetin, and on MS mineral salts with 10% (v/v) coconut milk. All cultures except cultures containing coconut milk were maintained in the light. Regeneration of gametophytes in A. undulatum, B. caespiticium, Plagiomnium affine and P. commune from protonemata, protonemata with abnormal buds, whole gametophytes, shoots of gametophytes and callus was observed on MS mineral salts, Knudson mineral medium, regular MS and Knudson media. Restored gametophytes were also obtained on MS mineral medium containing 10% (v/v) coconut milk from calluses or callus-type protonema. All cultures were maintained in the light.

10

Experimental hybridisation between X0 and XY chromosome races in the grasshopper Podisma sapporensis Shir. [Orthoptera, Acrididae]. I. Cytological analysis of embryos and F1 hybrids

67%

Bugrov A. G. , Warchalowska-Sliwa E. , Sugano Y. , Akimoto S.

Folia Biologica

|

2004

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tom 52

|

nr 1-2

11

Analysis of somatic chromosome numbers in doubled haploid lines of hexaploid Triticale [xTriticosecale Witt.]

59%

Sobieszczanska A.

Acta Biologica Cracoviensia. Series Botanica

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2000

|

tom 42

|

nr 1

EN

Somatic chromosome number was investigated in twenty doubled haploid (DH) lines derived from anther culture of two triticale cultivars. Nine lines have 42 chromosomes, the rest ranging from 39 to 43. The majority of aberrants (90%) are hypoaneuploids (2n = 39 to 41), but within the KR424 line both hypo- (2n = 39 to 41) and hyperaneuploid (2n = 43) chromosome numbers were identified. The observed frequency of seedlings with aneuploid chromosome numbers within several unstable lines varied from 4% to 31.2%.

12

Analiza molekularna i cytologiczna oraz ocena niektorych cech ilosciowych mieszancow miedzygatunkowych Avena sativa L. x Avena fatua L.

51%

Chrzastek M. , Paczos-Grzeda E.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2003

|

tom 230

387-397

13

Stabilnosc somatycznej liczby chromosomow u pszenzyta oktoploidalnego

42%

Kluska T. , Pilch J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

1995

|

tom 195-196

49-53

14

Analiza cytologiczna wysokobialkowych i wysokolizynowych rewertantow pszennych otrzymanych z pszenzyta heksaploidalnego

42%

Pilch J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

1995

|

tom 195-196

73-80