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EN
Septins belong to GTPases that are involved in vital cellular activities, including cytokinesis. Although present in many organisms, they are yet to be isolated from Aedes albopictus. This study reports for the first time on a serendipitous isolation of a partial septin sequence from Ae. albopictus and its developmental expression profile. The Ae. albopictus partial septin sequence contains 591 nucleotides encoding 197 amino acids. It shares homology with several insect septin genes and has a close phylogenetic relationship with Aedes aegypti and Culex quinquefasciatus septins. The Ae. albopictus septin fragment was differentially expressed in the mosquito's developmental stages, with an increased expression in the adults.
EN
According to many reports, the endosperm of Arabidopsis thaliana (L.) Heynh. follows Nuclear-type development whereby the primary endosperm nucleus divides without cytokinesis, ultimately to produce a peripheral, multinuclear endosperm tissue. However, some features of endosperm development in seeds collected from populations in central South Carolina reveal striking differences from the Nuclear type of other Brassicaceae. Nuclei produced by the first division of the central or slightly chalazal primary endosperm nucleus quickly migrate to opposite poles on the longitudinal axis, where the chalazal nucleus immediately becomes enshrouded with dense cytoplasm. Divisions from that point onward are free-nuclear and frequent in the micropylar portion, which enlarges markedly as it becomes multinucleate. The chalazal endosperm is sometimes positioned in a different focal plane as a result of curvature of the immature seed toward amphitropous form. It does not enlarge appreciably and accumulates only 2-8 nuclei before it gradually degenerates, persisting until digested during maturation of the developing embryo. Thus, the functional endosperm is produced primarily by the micropylar chamber.
EN
Four human colon adenocarcinoma cell line variants with different metastatic po­tential were used to examine whether a correlation exists between actin level, state of actin polymerization and invasiveness of tumour cells. Monomeric (G), total (T) and filamentous (F) actin were determined in the cytosolic fraction of these cells. A statistically significant decrease in G actin level and increase in the state of actin polymerization (measured by F:G actin ratio) were found in the cytosol of three cell variants with higher metastatic potential and invasiveness (EB3, 3LNLN, 5W) compared with the parental cell line (LS180). Our experimental data lead to the conclusion that there is a correlation between the metastatic capacity of human colon adenocarcinoma cells and the state of actin poly­merization.
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