Wyniki wyszukiwania - Biblioteka Nauki

1

Importance of explant size and origin for cryopreservation of Rosa pomifera “Karpatia” shoot tips by a droplet vitrification method

100%

Kwasniewska E. , Dziedzic E. , Pawlowska B.

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2015

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tom 96

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nr 1

2

Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocyte retrieval and IVF outcomes

100%

Kuc P. , Kuczynska A. , Stankiewicz B. , Sieczynski P. , Matysiak J. , Kuczynski W.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 1

84-88

3

Development and evaluation of modified cryopreservation for long-term storage of Blastocystis subtypes 1–3 and 6

100%

Karamati S. A. , Aghdaei H. A. , Niyyati M. , Yadegar A. , Haghighi A. , Tabaei S. J. S. , Mirjalali H. , Zali M. R.

Acta Parasitologica

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2020

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tom 65

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nr 2

4

Changes in cell physiology at the stage of dehydration in a cryopreservation protocol

100%

Domzalska L. , Kedracka-Krok S. , Mikula A. , Rybczynski J. J.

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2015

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tom 96

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nr 1

5

Ejaculation malfunctions on the day of oocyte pick up for IVF/ICSI: A report of four cases

100%

Javed A. , Ls A. , Pathangae V. G. , Roy A. , Ganguly D.

International Letters of Natural Sciences

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2015

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tom 48

EN

The procedure of in-vitro fertilization (IVF) is sometime physically stressful and emotional turmoil on the couples undergoing treatment. The male partner is obliged to produce semen for injection/insemination with the oocytes. Unfortunately, some men are ill-fated, not capable to reproduce semen on the day of oocyte pickup.

6

Characterization of human hepatocytes isolated from non-transplantable livers

100%

Laba A. , Ostrowska A. , Patrzalek D. , Paradowski L. , Lange A.

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nr 5

7

Cryopreservation of embryogenic tissues: a review

86%

Kulus D. , Zalewska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 1

8

Cryo-injury in algae and the implications this has to the conservation of micro-algae

86%

Day J. G. , Fleck R. A.

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nr 1

EN

As with all products and processes exploiting biological materials algal biotechnology has an absolute requirement for stable, function-fulfilling master stockcultures. Conventionally microalgae and cyanobacteria are maintained by serial transfer with inocula (2.5-25% v/v) being transferred to fresh medium and the culture being held under controlled environmental conditions. This method is satisfactory for many algal taxa, but by its nature cannot provide an absolute guarantee of phenotypic or genotypic stability. Cryopreservation at ultra-low temperatures (> -130oC) is the only methodology that can provide this level of security to master stock-cultures; however, many algae are recalcitrant to cryopreservation with low or no survival. This review explores the reasons of this cryo-recalcitrance on the application of conventional colligative, two-step cryopreservation protocols and points towards the options available to enhance postcryopreservation viability.

9

Cryopreservation of in vivo matured pig oocytes by OPS vitrification

86%

Gajda B. , Smorag Z.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

10

Embryo transfer in swine – an indispensable key for the application of reproductive techniques

86%

Brussow K.-P. , Ratky J. , Antosik P. , Kempisty B. , Jaskowski J. M.

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2018

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tom 21

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nr 3

11

The effect of breed on freezability of semen of fancy fowl

86%

Siudzinska A. , Lukaszewicz E.

Animal Science Papers and Reports

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2008

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tom 26

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nr 4

EN

A simple cryopreservation method described in 1995 by Tselutin et al. was used for freezing the semen of four fancy fowl breeds: White Crested Black Polish (WCBP), Greenleg Partridge (GP),Italian Partridge (IP) and Black Minorca (BP). The differences in quality (ejaculate volume,osmotic pressure, sperm concentration and morphology) of fresh semen between evaluated breeds were observed, as well as the differences in semen freezability. The freezing-thawing process caused significant (P≤0.01) decrease in percentage of live, normal spermatozoa, with coincident increase in percentage of dead spermatozoa and spermatozoa with acrosome defect. In relation to the fresh semen, the number of live, normal spermatozoa that survived cryopreservation procedure constituted 18.1% in WCBP, 25.1% in GP, 26.2% in IP and 33.6% in BM semen.

PL

Badano przydatność prostej metody mrożenia opracowanej i opisanej w 1995 r. przez Tselutina i współautorów do zamrożenia nasienia kogutów czterech amatorskich ras kur: czubatki polskiej (White Crested Black Polish – WCBP), zielononóżki kuropatwianej (Greenleg Partridge – GP), włoszki kuropatwianej (Italian Partridge – IP) i minorki czarnej (Black Minorca – BM). Stwierdzono różnice w jakości nasienia świeżego (objętość ejakulatów, ciśnienie osmotyczne, koncentracja i morfologia plemników) między kogutami ocenianych ras, a także różnice między rasami w podatności nasienia na mrożenie. Proces ekwilibracji nasienia w obecności 6% DMA oraz jego zamrożenie-rozmrożenie powodowały istotny (P≤0,01) spadek liczby plemników żywych, prawidłowo ukształtowanych, przy równoczesnym wzroście liczby plemników martwych lub z uszkodzonym akrosomem. Bez względu na rasę kogutów, mrożenie nasienia zastosowaną metodą powodowało spadek liczby plemników żywych prawidłowo ukształtowanych, których udział w stosunku do udziału w nasieniu świeżym stanowił w przypadku czubatki polskiej 18,1%, zielononóżki kuropatwianej 25,1%, włoszki kuropatwianej 26,2% oraz 33,6% w przypadku minorki czarnej.

12

A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation

86%

Makowski D. , Rybczynski J. J. , Mikula A.

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tom 84

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nr 3

EN

Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.

13

Evaluation of two prototype directional freezing methods and a 2ml flattened straw for cryopreservation of boar semen

86%

Puglisi R. , Bornaghi V. , Severgnini A. , Vanni R. , Montedoro M. , Galli A.

Animal Science Papers and Reports

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2017

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tom 35

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nr 4

EN

Based on previous assessments on stallions, 40 ejaculates of 20 Duroc boars were split and evenly frozen with a conventional vapour freezing method and two directional, drum and directional, prototype methods using commercial extenders and relative standard procedures. The directional prototype was provided with a double internal setup that allowed the positioning of experimental 2ml flat straws with 1 billion sperm (Flat) in a fixed support, or both classical 0.5 ml paillettes with 250 million spermatozoa and flats in a rotating drum designed so as to ensure a more uniform heat exchange. Preliminary tests for individuation of the most appropriate thawing rate showed beneficial effects (P≤0.05) of thawing the sperm at 50°C for 13 s when compared to 42°C for 20 s, in terms of total motility (42.8±8.4% and 35.6±6.8%, respectively). With regard to freezing/packaging methods, major improvements (P≤0.05) were shown for the drum method with paillettes for total motility (38.6±14.2%) assessed immediately after thawing, when compared with the conventional (29.4±13.3%) and the directional methods with flats (30.2±12.8%), and for total motility (P≤0.01) assessed following incubation for 120 min at 37°C after thawing (24.8±11.6%) with respect to the conventional method (15.6±10.9%). Despite the statistical non-significance of results, both the prototype freezing approaches using the experimental flat straw showed some improvements in functional parameters assessed by cytofluorometry when compared to the conventional method.

14

Viability of bovine embryos after different equilibration vitrification treatment

86%

Smorag Z. , Gajda B.

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nr 1-2

15

The influence of semen cryopreservation on the release of some enzymes from Siberian sturgeon [Acipenser baerii] and sterlet [Acipenser ruthenus] spermatozoa

86%

Sarosiek B. , Ciereszko A. , Rzemieniecki A. , Domagala J. , Glogowski J.

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tom 12

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nr 1

EN

The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) was frozen without cryoprotector (at -79°C) or cryopreserved with methanol as the cryoprotector. The activity of arylsulfatase (AS), acid phosphatase (AcP), β-N-acetylglucosaminidase (NAGase), and protein concentration was determined. The protein concentration and enzymatic activities in supernatant obtained after cryopreservation were higher than in milt plasma, but they were lower than that in the material obtained after freezing at -79°C. The protein and enzymatic leakage of sterlet spermatozoa was statistically higher in supernatants that had been frozen at-79°C than in those that had undergone cryopreservation. Differences in the protein and AS leakage the Siberian sturgeon supernatants were also observed.

PL

Nasienie pobrane od 19 osobników jesiotra syberyjskiego (Acipenser baerii) i 10 - sterleta (A. ruthenus) zamrożono w -79°C bez obecności krioprotektora oraz poddano kriokonserwacji z metanolem jako krioprotektorem. Koncentrację oraz ruchliwość użytego w eksperymencie nasienia zamieszczono w tabeli 1. W świeżej plazmie nasienia oraz supernatantach uzyskanych po odwirowaniu zamrożonego materiału oznaczono zawartość białka oraz aktywność arylosulfatazy (AS), kwaśnej fosfatazy (AcP) i β-N-acetyloglukozoaminidazy (NAGase) (tabela 2). Oznaczone parametry w supernatancie uzyskanym po kriokonserwacji były statystycznie istotnie wyższe niż w plazmie nasienia i jednocześnie niższe niż supernatancie po mrożeniu bez obecności metanolu (tabela 2). Wykazano również istnienie korelacji pomiędzy „wyciekiem" arylosulfatazy i koncentracją plemników w materiale uzyskanym po mrożeniu w -79°C (rys. 1). W supernatancie po kriokonserwacji nie obserwowano istnienia takiej zależności (rys. 2).

16

Elaboration of optimal conditions for micropropagation and cryopreservation of garlic [Allium sativum L.]

86%

Makowska Z. , Kotlinska T.

Vegetable Crops Research Bulletin

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2001

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tom 54

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nr 1

17

Obtaining chicken chimeras after injecting cryopreserved blastoderm cells into the subgerminal cavity of recipient embryos

86%

Pokorny P.

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tom 20

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nr 1

18

The use of flow cytometry for the investigation of viability of heart valve - derived fibroblasts

86%

Wilczek P. , Szydlowska I. , Lotysz D. , Religa Z.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

19

Cryopreservation of plant cell cultures

86%

Van Iren F. , Schrijnemakers E. W. M. , Reinhoud P. J. , Kijne J. W.

Biotechnologia

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1996

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nr 1

20

A simple and efficient method for cryopreservation of embryogenic triticale calli

86%

Sowa S. , Oleszczuk S. , Zimny J.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 2

EN

Conventional breeding methods are now supplemented by modern in vitro techniques. However, long term maintenance of cultures has many disadvantages. It incurs risk of loss through microbial contamination, somaclonal variation or human error, but above all the regeneration capacity can decrease gradually during extended maintenance. Cryopreservation as a method of long term storage of biological material without genetic alteration was adapted for embryogenic triticale calli preservation. Callus of both winter and spring genotypes were successfully cryopreserved. The best viability rates (80-85 %) were achieved with 6 weeks old winter genotypes treated with cryoprotective solution containing DMSO. This simple and efficient method of cryopreservation requires no special devices for controlled freezing and can be easily adapted for other cereals.