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Isolation and characterisation of crocodile and python ovotransferrins

100%

Ciuraszkiewicz J. , Olczak M. , Wątorek W.

2007

tom 54

nr 1

175-182

Transferrins play a major role in iron homeostasis and metabolism. In vertebrates, these proteins are synthesised in the liver and dispersed within the organism by the bloodstream. In oviparous vertebrates additional expression is observed in the oviduct and the synthesised protein is deposited in egg white as ovotransferrin. Most research on ovotransferrin has been performed on the chicken protein. There is a limited amount of information on other bird transferrins, and until our previous paper on red-eared turtle protein there was no data on the isolation, sequencing and biochemical properties of reptilian ovotransferrins. Recently our laboratory deposited ten new sequences of reptilian transferrins in the EMBL database. A comparative analysis of these sequences indicates a possibility of different mechanisms of iron release among crocodile and snake transferrin. In the present paper we follow with the purification and analysis of the basic biochemical properties of two crocodile (Crocodilus niloticus, C. rhombifer) and one snake (Python molurus bivittatus) ovotransferrins. The proteins were purified by anion exchange and hydrophobic chromatography, and their N-terminal amino-acid sequences, molecular mass and isoelectric points were determined. All three proteins are glycosylated and their N-glycan chromatographic profiles show the largest contribution of neutral oligosaccharides in crocodile and disialylated glycans in python ovotransferrin. The absorption spectra of iron-saturated transferrins were analysed. Iron release from these proteins is pH-dependent, showing a biphasic character in crocodile ovotransferrins and a monophasic type in the python protein. The reason for the different types of iron release is discussed.

Comparison of meat quality of selected exotic animals species

100%

Balowski M. , Sobczak M. , Zochowska-Kujawska J. , Pytel-Zajac O. , Niedzwiedz M.

Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica

2015

tom 36

„Что само в очесях являлося, то и писалося…” Князя Николая Радзивилла Cиротки описание диковинных животных в его „Путешествии в Святую Землю”

100%

Bolek A.

2017

tom 165

57-67

The paper analyzes descriptions of three exotic beasts (hippopotamus, crocodile and chameleon) in Count Nicholas Radzivill’s travelogue Pilgrimage to the Holy Land and Egypt. Giving his first-hand impressions of the world totally unknown for the Europeans, the author endeavors to factually represent it basing on his own previous experience. This mode of representation, sheer devoid of mythological and fictional dimensions, is also applied to the fauna which was the object of the Author’s close attention. Taken against the background of the contemporary, predominantly mythological (antique and medieval) images of the beasts, the study of the representational mode applied by The Orphan allows to look upon the Polish traveler as areliable, inquisitive and sensitive observer.

W artykule przeanalizowano opisy trzech egzotycznych zwierząt (hipopotama, krokodyla i kameleona) zdiariusza podróży do Ziemi Świętej iEgiptu księcia Mikołaja Radziwiłła Sierotki. Jego autor, opisując nowy — nieznany Europejczykowi — świat, starał się przedstawić go faktograficznie zperspektywy własnych doświadczeń ispostrzeżeń. Ten sposób przekazu, odcinający się od wymysłów imitów, dotyczy również świata zwierząt, których Radziwiłł był bacznym obserwatorem. Rozpatrzenie prezentacji przez Sierotkę trzech „bestii” na tle panujących wówczas mitycznych — antycznych iśredniowiecznych — wyobrażeń onich pozwala ujrzeć wrelacjach wiarygodnego, dociekliwego, lecz także wrażliwego autora.

New observations on Micropleura australiensis [Nematoda, Dracunculoidea], a parasite of crocodiles in Australia

80%

Moravec F. , Spratt D. V. , Kay W. R.

nr 4

Scanning electron microscopy examination of male and female specimens of Micropleura australiensis Moravec, Kay et Hobbs, 2004 (Nematoda, Dracunculoidea) from the peritoneal cavity of the freshwater crocodile Crocodylus johnsoni of the Ord River in Western Australia revealed some previously unreported morphological features. The male, first studied by SEM, possesses the same number (14) of cephalic papillae as the gravid female, but these are comparatively larger and somewhat differently arranged; four slit-like depressions located near the inner base of male dorsolateral and ventrolateral cephalic papillae of the external circle are present. The inner margin of the female oral aperture is smooth, without papilla-like formations. The male caudal end is provided with a right-side ventral ala. The transverse cuticular striae on the male ventral surface bear minute ornamentations (rows of papilla-like formations) in the pre-and postcloacal region. For the first time, M. australiensis is reported from wild and farmed Crocodylus johnsoni and saltwater crocodile C. porosus (new host record) from four localities in the Northern Territory, Australia.