Wyniki wyszukiwania - Biblioteka Nauki

1

Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS

100%

Wang Y. , He Y. , Wang X. , Wen C. , Cao J.

Acta Chromatographica

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2024

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tom Vol. 36, no. 3

179--185

EN

In this work, a UPLC-MS/MS assay was established for the determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma. ACQUITY UPLC BEH C18 column was employed for chromatographic separation with the mobile phase comprised acetonitrile-10 mmol L1 ammonium acetate aqueous solution (0.05% aqueous ammonia) using gradient elution. Midazolam was used as internal standard (IS). Electrospray ionization (ESI) in positive-ion mode with reaction monitoring (MRM) was used for quantitative analysis. The calibration curves for morphine, codeine, thebaine, papaverine and noscapine demonstrated good linearity (r > 0.995) in the range of 5–500 ng mL1 for morphine and codeine, and 1–100 ng mL1 for thebaine, papaverine and noscapine. The intra-day and inter-day precisions of morphine, codeine, thebaine, papaverine and noscapine were within 15%, the intra-day and inter-day accuracies were 89–114%, the recovery was better than 65%, and the matrix effects were 96–112%. The developed UPLC-MS/MS assay was successfully applied in the pharmacokinetics of papaverine and noscapine.

2

Simultaneous RP-IP-HPLC assay of theophylline, phenobarbital, codeine, and ephedrine in a suppository

100%

Jankovics P. , Németh T. , Németh-Palotás J. , Kőszegi-Szalai H.

Acta Chromatographica

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2010

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tom Vol. 22, no. 4

527--538

EN

A reversed-phase ion-pairing liquid chromatographic (RP-IP-HPLC) method for simultaneous assay of theophylline (TH), phenobarbital (PB), codeine (CD), and ephedrine (EP) in an extemporaneous (magistral) suppository was developed and validated and used to investigate a reported serious adverse event. Samples were dissolved in dichloromethane and extracted by two-step liquid-liquid extraction with acetate buffer (pH 5.0) and, subsequently, 0.1 M NaOH solution. Separation was performed on an end-capped C 18 silica column with stepwise gradient elution. Sample preparation and chromatographic conditions were optimized on the basis of the p K a values of the analytes. Freedom from interference from the excipients, linearity ( r 2 > 0.999 for all the active ingredients), range (0.01–0.08 mg mL -1 for TH, CD, and EP; 0.0025–0.02 mg mL -1 for PB), intra-day and inter-day precision, and accuracy (recovery >95% for TH, CD, and EP and >90% for PB) of the method were demonstrated. Non-compliance of the examined product was confirmed.

3

Compartmental model of the pharmacokinetics of drugs excreted by bile or by non-renal mode

84%

Jastrzebski J. P. , Urniaz R.

Bio-Algorithms and Med-Systems

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2011

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tom Vol. 7, no. 2

31--37

EN

The theoretical biology analysis, uses mathematical and physical models and bioinformatics tools in practical medicine. The analysis in silico reduces time and costs of new designed drugs and provides wider spectrum of information for exploration. Moreover, bioinformatics analysis of genome and proteome, especially with the use of microarray allows specifying diagnosis and therapy. The methods enabling precise preparation of therapy by means of accurate medicine dosage are being currently searched, such an approach permits to increase the effectiveness of therapy, eradicate side effects (i.e. medicine overdosing), reduce the total cost of therapy and cut down its interval. The elaborated model involves the partition of human body into compartments having different physical features: weight and volume, or physiological character: metabolic efficiency, penetration rate of drug. Additional parameters can be introduced to the model: Wa/b, Wwzb and λ. Due to the nature of simulation each individual case can be run with high precision. The model is implemented in the GROW_4 virtual environment which is accessible free of charge on the websites: www.sitcome.vacau.com or www.uwm.edu.pl/bioinfo (in science/projects/grow).

4

Determination of morphine and codeine in serum after poppy seed consumption using gas chromatography-mass spectrometry

84%

Mátyus M. , Kocsis G. , Boldis O. , Karvaly G. , Magyari E. , Fűrész J. , Gachályi A.

Acta Chromatographica

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2012

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tom Vol. 24, no. 3

351--365

EN

A sensitive method was developed for the determination of free morphine and codeine in blood. This method is suitable for detection of the ingestion of illicit drugs. This test is applicable for monitoring therapeutic doses as well as stand up in the court of law. For the test we use with deuterium tagged morphine and codeine. We tag the blood specimens with deuterium prior to testing. The active ingredients (morphine and codeine) are extracted by solid phase extraction, and eventually derivatized with pentafluoro propionic anhydride (PFPA). The resulting compounds are stable and have good chromatographic characteristics. The Gas-chromatograph coupled Mass-spectrometer (GC/MS) system is used to determine the concentration of the resulting compounds using or/in the selective ion mode (SIM). The results of the target and qualifier ions were 414, 577, 361 in the case of morphine, and 282, 445 in the case of codeine. During our measurements, the graph was linear between 1 and 100 ng / mL. All the results of the qualifications were in line with the German Standards DIN 32645, which were checked with the B.E.N. program.

5

Determination of morphine and codeine in serum and saliva by gas chromatography-mass spectrometry

67%

Janowska E. , Piekoszewski W.

Chemia Analityczna

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2001

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tom Vol. 46, No. 6

857-864

EN

The GC-MS method of morphine and codeine determination in serum and saliva was developed and validated. The sample preparation consists of enzymatic hydrolysis, deprotei-nisation, solid phase extraction and derivatisation. As the intrinsic standards D3-morphine and D3-codeine were used. Detection limit for morphine and codeine was 4 and 5 ng ml(-1); quantification limit was 14 and 15 ng ml(-1) respectively. The range of quantification was 20-1000 ng ml"1 and the recovery ranged 50-60%. For checking the applicability of the elaborated method, morphine and codeine concentrations were measured in serum and saliva samples taken from 10 addicts. The method allowed to measure morphine in saliva and may be used for monitoring taking home made heroin and detoxification process.

PL

Przedstawiono metodę oznaczania morfiny i kodeiny w surowicy krwi oraz w ślinie za pomocą chromatografii gazowej sprzężonej ze spektrometrią mas. Ekstrakcję analitów, po wcześniejszej hydrolizie enzymatycznej, prowadzono w układzie ciecz-ciało stałe. Morfinę i kodeinę oznaczano, po derywatyzacji te(trimetylosililo)trifluoracetamidem, metodą GC-MS stosując jako substancje wzorcowe związki deuterowane. Wykrywalność metody wynosiła 4 i 5 ng ml'1 a oznaczalność 14 i 15 ng ml'1 odpowiednio dla morfiny i kodeiny. Wydajność ekstrakcji badanych związków wahała się od 50-60%. Opracowaną metodę zastosowano do pomiaru stężenia morfiny i kodeiny u 10 osób uzależnionych od pochodnych alkaloidów opium ("kompotu").

6

Jakosciowe oznaczanie morfiny, kodeiny i tebainy w makowinach metoda HPLC do celow hodowlanych

59%

Michalski K.

Rośliny Oleiste - Oilseed Crops

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2004

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tom 25

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nr 2

621-626

7

Wstępne badania nad optymalizacją warunków indukowania mutacji dla uzyskania nowej zmienności u maku (Papaver somniferum L.)

51%

Walkowiak M. , Ogrodowczyk M.

Rośliny Oleiste - Oilseed Crops

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2013

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tom 34

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nr 2

PL

Celem prowadzonych badań było znalezienie optymalnych metod indukowania mutagenezy dla uzyskania nowej zmienności głównych alkaloidów maku: morfiny, tebainy i kodeiny. Materiał do badań stanowiły genotypy linii hodowlanych pochodzące z rekombinantów różnych form maku wysokomorfinowego z kolekcji IHAR-PIB oraz wysokomorfinowa odmiana Lazur. Badania prowadzono w trzech sezonach wegetacyjnych. Zastosowano czternaście sposobów indukowania mutagenezy stosując zróżnicowane stężenia mutagenu chemicznego i ultradźwięków oraz różny czas ekspozycji mutagenem. Nasiona traktowano metanosulfonianem etylu (EMS) oraz ultradźwiękami (UD) w celu ułatwienia wnikania substancji mutagennej do nasion. Stężenia mutagenu wynosiły: 0,4; 0,6; 0,8; 1,0; 1,2; 1,4 i 1,6% EMS. Nasiona wytypowanych genotypów maku pozostawały w roztworze trzy lub cztery godziny w zależności od stężenia mutagenu. Przeprowadzono również badania wpływu ultradźwięków na zwiększenie działania mutagenezy chemicznej na nasiona maku odmiany Lazur. Czas ekspozycji nasion na działanie ultradźwięków wynosił 5 lub 25 minut. Po zdekantowaniu roztworu nasiona wymywano pod bieżącą wodą i osączano na bibule. Nasiona poddane mutagenezie i linie kontrolne rozmnażano w szkółkach selekcyjnych. Zastosowanie mutagenezy pozwoliło na uzyskanie zmienności zawartości morfiny, kodeiny i tebainy. Poszerzenie zmienności zawartości alkaloidów w makowinach maku potwierdza, że EMS jest wydajnym mutagenem chemicznym stosowanym do tworzenia nowych zmienności.

EN

The aim of the studies was to identify optimum method for inducing mutagenesis to obtain new variation alkaloid in poppy. The study material consisted of breeding lines derived from high-morphine recombinants of various forms of poppy from the IHAR-PIB collection, and the high-morphine Lazur variety. The studies were carried out over three vegetation seasons. A total of 14 methods of mutagen treatment were employed, using varying concentrations of the chemical and ultrasounds, and different mutagen exposure times. Seeds were treated with ethyl methanesulphonate (EMS) and ultrasounds (US) in order to facilitate the penetration of mutagenic substances into the seeds. Mutagen concentrations were: 0.4; 0.6; 0.8; 1.0; 1.2; 1.4 and 1.6% EMS. Seeds of selected poppy genotypes were kept in the solution for three or four hours depending on mutagen concentration. The study also involved investigation of the effect of ultrasound treatment on the increase in effectiveness of chemical mutagenesis in poppy seeds of the Lazur variety. The duration of seed exposure to ultrasound treatment was 5 or 25 minutes. Following decantation, the seeds were washed under running water and dried on tissue paper. The seeds that had been subjected to mutagen treatment and control lines were then propagated in selection nurseries. It was possible to achieve higher variation in the content of morphine, codeine or thebaine. Extended range of variation in alkaloid content in poppy capsules demonstrates that EMS is an efficient chemical mutagen suitable for the development of new genotypes.