Wyniki wyszukiwania - Biblioteka Nauki

1

Isolation and characterization of novel human short-chain dehydrogenase/reductase SCDR10B which is highly expressed in the brain and acts as hydroxysteroid dehydrogenase*

100%

Huang C. , Wan B. , Gao B. , Hexige S. , Yu L.

Acta Biochimica Polonica

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2009

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tom 56

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nr 2

279-289

EN

Hydroxysteroid dehydrogenase belongs to the subfamily of short-chain dehydrogenases/reductases (SDR), and 11-β-hydroxysteroid dehydrogenase catalyzes the interconversion of inactive glucocorticoids (cortisone in human, dehydrocorticosterone in rodents) and active glucocorticoids (cortisol in human, corticosterone in rodents). We report here the cloning and characterization of a novel human SDR gene SCDR10B which encodes a protein with similarity to 11β-hydroxysteroid dehydrogenase 1. SCDR10B was isolated from a human brain cDNA library, and was mapped to chromosome 19p13.3 by browsing the UCSC genomic database. It contains an ORF with a length of 858 bp, encoding a protein with a transmembrane helix and SDR domain. Its molecular mass and isoelectric point are predicted to be 30.8 kDa and 10.3 kDa, respectively. SCDR10B protein is highly conserved in mammals and fish. Phylogenetic tree analysis indicated that SCDR10B stands for a new subgroup in the 11β-hydroxysteroid dehydrogenase family. Northern blot analysis showed that SCDR10B was highly expressed in brain, and a strong expression signal was detected in hippocampal neurons by immunohistochemical analysis. RT-PCR and immunohistochemical analysis showed that SCDR10B was up-regulated in lung-cancer cell lines and human lung cancer. SCDR10B can catalyze the dehydrogenation of cortisol in the presence of NADP+, and therefore it is a hydroxysteroid dehydrogenase.

2

Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9

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Liu S. , Huang C. , Li D. , Ren W. , Zhang H. , Qi M. , Li X. , Yu L.

Acta Biochimica Polonica

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2007

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tom 54

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nr 1

213-218

EN

We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

3

Almost associative operations generating a minimal clone

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Waldhauser T.

Discussiones Mathematicae - General Algebra and Applications

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2006

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tom 26

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nr 1

45-73

EN

Characterizations of 'almost associative' binary operations generating a minimal clone are given for two interpretations of the term 'almost associative'. One of them uses the associative spectrum, the other one uses the index of nonassociativity to measure how far an operation is from being associative.

4

The submaximal clones on the three-element set with finitely many relative R-classes

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Lehtonen E. , Szendrei Á.

Discussiones Mathematicae - General Algebra and Applications

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2010

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tom 30

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nr 1

7-33

EN

For each clone C on a set A there is an associated equivalence relation analogous to Green's R-relation, which relates two operations on A if and only if each one is a substitution instance of the other using operations from C. We study the maximal and submaximal clones on a three-element set and determine which of them have only finitely many relative R-classes.

5

Clausal relations and C-clones

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Vargas E.

Discussiones Mathematicae - General Algebra and Applications

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2010

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tom 30

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nr 2

147-171

EN

We introduce a special set of relations called clausal relations. We study a Galois connection Pol-CInv between the set of all finitary operations on a finite set D and the set of clausal relations, which is a restricted version of the Galois connection Pol-Inv. We define C-clones as the Galois closed sets of operations with respect to Pol-CInv and describe the lattice of all C-clones for the Boolean case D = {0,1}. Finally we prove certain results about C-clones over a larger set.

6

Plonowanie wierzby wiciowej - w świetle badań

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Sobczyk W.

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2007

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tom T. 10, spec. 2

547-555

PL

Celem badań była ocena wielkości plonowania pięciu klonów wierzby energetycznej z plantacji w Nadleśnictwie Brodła, woj. małopolskie. W artykule wykazano, że zarówno w 2003 r., jak i w 2006 r. uzyskano najwyższe plonowanie klonu nr 1056. Jednakże, ze względu na bardzo suchy rok 2003, plonowanie tego klonu było wówczas niższe (27 Mg/ha) niż w 2006 r. (35 Mg/ha). Wierzba Salix viminalis jest rośliną przystosowaną do wegetacji na każdym gruncie. Uprawa wierzby nie wymaga szczególnych zabiegów agrotechnicznych. Przez zakładanie i prowadzenie plantacji wierzby energetycznej można przyczynić się do rozwoju wsi, zwiększenia dochodów rolniczych i zmniejszenia bezrobocia na wsi. Szczególnie ważny jest fakt, że największe zapotrzebowanie na siłę roboczą przy uprawie plantacji wierzby notuje się w okresie zimowym, czyli w okresie najmniejszego zapotrzebowania na siłę roboczą w tradycyjnym rolnictwie.

EN

The aim of the investigations was the assessment of the size of harvest of five energetic basket willow clones at a plantation of the Forest Inspectorate Brodła in the Malopolska voivodeship. The article shows that both in 2003 and in 2006 the highest crops of clone No. 1056 were obtained. However, due to the drought, the harvest in 2003 was lower (27 t/hectare) than that of 2006 (35 t/hectare). The willow Salix viminalis is a plant, which easily adapts to vegetation on any soil. The plant growing does not require any special agrotechnical treatment. The energetic willow cultivation can contribute to economic development of the country and the improvement of the farmer's profits, as well as can help to reduce unemployment. The most important is the fact that the largest demand for manpower in the willow plantation is in winter, i.e. in the time when much less manpower is required in traditional agriculture.

7

Zmiany w epidemiologii inwazyjnych klonów MRSA w wybranych szpitalach w Polsce

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Michał Karyński M. , Jolanta Krzysztoń-Russjan J. , Paweł Grzesiowski P.

Medical Review

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2012

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nr 3

311-317

PL

Wstęp: Staphylococcus aureus jest patogenem, który może wywoływać szerokie spektrum chorób od względnie łagodnych zakażeń skóry do zagrażających życiu chorób układowych. Badanie przeprowadzono w celu oceny klonalności meticylinoopornych izolatów S. aureus (MRSA) w wybranych polskich szpitalach. Materiał i metoda: Kolekcja szczepów składała się ze 183 meticylinoopornych izolatów S. aureus (MRSA) i 4 meticylinowrażliwych izolatów S. aureus (MSSA) z lat 2002-2005. Izolaty pochodziły z 29 szpitali. Oczyszczony całkowity DNA z izolatów był użyty w amplifiacji regionu X genu spa. Następnie, sekwencjonowano amplikony i określano typy spa. Wyniki: W wyniku przeprowadzonej analizy izolatów wykazano istnienie 30 typów spa. Dwoma najczęściej występującymi typami izolowanymi w ciągu okresu badania były t037 i t052. Typy te należały, odpowiednio, do tzw. klonów brazylijsko- -węgierskiego i iberyjskiego. W okresie 4 lat obserwacji stwierdzono zanik typu spa t052 (z 43% w 2002 do 0% w 2005). Równolegle z zanikiem typu spa t052, odsetek typu spa t037 zwiększył się z 26% w 2002 do 47% w 2005. Wnioski: Zmiany w częstości występowania izolatów t052 i t037 wskazują, że klon iberyjski, wykazujący w większości wrażliwość na ko-trimoksazol, został wyparty przez klon brazylijsko-węgierski wykazujący w większości oporność na ko-trimoksazol. Zmiany w strukturze populacji MRSA w szpitalach wytypowanych do badania obrazują dynamiczne zmiany w epidemiologii klonów, najprawdopodobniej związane z presją selekcyjną antybiotyków stosowanych w terapii zakażeń szpitalnych.

EN

Introduction: Staphylococcus aureus is a pathogen that can cause a wide spectrum of diseases from relatively mild skin infections to life-threatening systemic disease. The study was conducted to assess the clonality of methicillin-resistant isolates of S. aureus (MRSA) in selected Polish hospitals. Material and Methods: A collection of strains consisted of 183 methicillin-resistant isolates of S. aureus (MRSA) and 4 methicillin susceptible isolates of S. aureus (MSSA) from 2002-2005. Isolates came from 29 hospitals. Purifid total DNA of isolates was used to amplify the region X of the gene spa. Subsequently, the amplicons were sequenced and spa types determined. Results: The analysis of isolates demonstrated the existence of 30 types of spa. The two most common types isolated during the study period were t037 and t052. These types belonged, respectively, to the so-called Brazilian-Hungarian and Iberian clones. During 4 years of observation period, the loss of spa type t052 was observed (from 43% in 2002 to 0% in 2005). In parallel with the disappearance of a spa type t052, a spa t037 percentage increased from 26% in 2002 to 47% in 2005. Conclusions: The changes in the frequency of occurrence of t052 and t037 isolates show that the Iberian clone, mostly showing susceptibility to cotrimoxazole, was supplanted by the Brazilian/Hungarian clone, mostly showing resistance to cotrimoxazole. The alterations in the structure of the MRSA population in the hospitals selected for the study represent dynamic changes in the epidemiology of clones, most probably related to the selection pressure of antibiotics used in the therapy of hospital infections.

8

New Medical Technology and Human Dignity

63%

Yotov S.

Dialogue and Universalism

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2015

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nr 1

251-263

EN

First I discuss the rights as unavoidable part of the human dignity. There are four possible relations: dignity has a wider extension, the volume of both is equivalent, dig-nity includes in itself a bundle of rights, or it is just a simple right. There are good rea-sons to support the last two, even the last position. Then I evaluate some of the chal-lenging innovations in the medical technology, if they are acceptable in front of this close connection. The focus falls on three topics: PGD, cloning, and fusion of human-animal cells. Using moral principles such as non discrimination and non instrumentali-zation I look for some normative framing.