Antiseptics are a group of substances widely used in ophthalmology for many years. They are well established, especially in the prevention of perioperative infections. For decontamination in ophthalmology, a 5% povidone-iodine solution is used as a standard for the conjunctival sac. It is considered the most effective preoperative antisepsis. In patients with an allergy to iodine, a solution of 0.05% chlorhexidine is recommended. There are also attempts to use other antiseptics and antibiotics for perioperative prophylaxis. Recently, there has also been interest in potential use antiseptics for the treatment of conjunctivitis.