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1
Content available remote Chitinolytic activity of bacteria and fungi isolated from shrimp exoskeletons
100%
Swiontek-Brzezinska M. , Lalke-Porczyk E. , Donderski W.
Oceanological and Hydrobiological Studies
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2007
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tom Vol. 36, No.3
101-111
EN
Microbiological analysis of shrimp exoskeletons demonstrated considerable differences in abundance of heterotrophic bacteria and fungi. The number of heterotrophic bacteria was greater by two orders of magnitude than that of fungi. The survey, however, did not reveal significant differences in abundances of bacteria and fungi in samples coWected during different months of the survey. The percent contribution of chitinolytic fungi in shrimp exoskeletons was greater than that of bacteria that hydrolyze chitin. The activity of chitinase bacteria was always higher than fungi. Chitinases produced by bacteria demonstrated the highest level of activity at 40°C and pH = 8. In contrast, fungal chitinases showed the highest activity at 50°C and pH = 5.
2
The effect of long-term mineral fertilization on the mycoflora and chitinolytic activity of agricultural soils
86%
Popowska-Nowak E. , Koval E. Z. , Kharkevich E. S.
Polish Ecological Studies
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1995
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tom 21
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nr 1
3
Chitinolytic activity of bacteria and fungi isolated from shrimp exoskeletons
86%
Swiontek-Brzezinska M. , Lalke-Porczyk E. , Donderski W.
Oceanological and Hydrobiological Studies
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2007
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tom 36
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nr 3
4
Content available remote Effects Of Herbicides And Fungicides On The Soil Chitinolytic Activity. A Molecular Docking Approach
72%
Vlădoiu D. L. , Filimon M. N. , Ostafe V. , Isvoran A.
Ecological Chemistry and Engineering S
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2015
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tom 22
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nr 3
439-450
EN
A molecular docking study was undertaken using the programs SwissDock and PatchDock to assess the interactions of the bacterial chitinases belonging to the GH18 and GH19 families with two herbicides (chlorsulfuron and nicosulfuron) and two fungicides (difenoconazole and drazoxolon). Both molecular docking programs predict that all considered pesticides bind to the active sites of chitinases produced by soil microorganisms. There are correlations for predicted binding energy values for receptor-ligand complexes obtained using the two programs consolidating the prediction of the chitinases-pesticides interactions. The interactions of chitinases with pesticides involve the same residues as their interactions with known inhibitors suggesting the inhibitory potential of pesticides. Pesticides interact stronger with chitinases belonging to the GH18 family, their active sites reflecting higher polarity than those of the GH19 chitinases. Also, herbicides reveal a higher inhibitory potential to bacterial chitinases than fungicides.
5
Content available remote Effects of herbicides and fungicides on the soil chitinolytic activity. A molecular docking approach
72%
Vlădoiu D. L. , Nicoleta F. M. N. , Ostafe V. , Isvoran A.
Ecological Chemistry and Engineering. S
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2015
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tom Vol. 22, nr 3
439--450
EN
A molecular docking study was undertaken using the programs SwissDock and PatchDock to assess the interactions of the bacterial chitinases belonging to the GH18 and GH19 families with two herbicides (chlorsulfuron and nicosulfuron) and two fungicides (difenoconazole and drazoxolon). Both molecular docking programs predict that all considered pesticides bind to the active sites of chitinases produced by soil microorganisms. There are correlations for predicted binding energy values for receptor-ligand complexes obtained using the two programs consolidating the prediction of the chitinases-pesticides interactions. The interactions of chitinases with pesticides involve the same residues as their interactions with known inhibitors suggesting the inhibitory potential of pesticides. Pesticides interact stronger with chitinases belonging to the GH18 family, their active sites reflecting higher polarity than those of the GH19 chitinases. Also, herbicides reveal a higher inhibitory potential to bacterial chitinases than fungicides.
PL
W celu oceny oddziaływań chitynaz bakteryjnych należących do rodziny GH18 i GH19 z dwoma herbicydami (chlorosulfuron i nikosulfuron) i dwoma fungicydami (difenokonazol i drazoxolon) przeprowadzono badania dokowania molekularnego za pomocą programów SwissDock i PatchDock. Oba programy dokowania molekularnego przewidują, że wszystkie badane pestycydy wiążą się z miejscami aktywnymi chitynaz, wytwarzanych przez drobnoustroje glebowe. Istnieją korelacje dla przewidywanych wartości energii wiązania kompleksów receptor-ligand uzyskanych za pomocą dwóch programów, potwierdzając przewidywane interakcje między chitynazami a pestycydami. Oddziaływania chitynaz z pestycydami dotyczą tych samych reszt, jak ich interakcje ze znanymi inhibitorami, co sugeruje hamujące zdolności pestycydów. Pestycydy oddziałują silniej z chitynazami należącymi do rodziny GH18 - ich miejsca aktywne wykazują wyższą polarność niż te z chitynaz GH19. Ponadto, herbicydy wykazują wyższe zdolności hamujące wobec chitynaz bakteryjnych w porównaniu do fungicydów.
6
Proteolytic and chitinolytic activity of the Pseudomonas isolated from within sporocarps of Cantharellus cibarius
72%
Dahm H. , Wrotniak W.
Bulletin of the Polish Academy of Sciences. Biological Sciences
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2003
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tom 51
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nr 3
7
Utilization of shrimp-shell waste as a substrate for the activity of chitinases produced by microorganisms
58%
Swiontek B. M. , Walczak M. , Lalke-Porczyk E. , Donderski W.
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tom 19
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nr 1
177-182
EN
Chitinolytic microorganisms such as bacteria, fungi, and actinomycetes, were isolated from the water and bottom sediments of Lake Chełmżyńskie and the soil of its watershed. The impact of various forms of chitin on the chitinolytic activity of these microorganisms was investigated. Bacteria isolated from the analyzed environments exhibited the highest chitinolytic activity in the presence of colloidal chitin. High activity was also observed in a culture medium containing shrimp shells. The analysis of chitinolytic activity of actinomycetes demonstrated that strains isolated from the soil had considerably higher activity levels than those isolated from lacustrine water and bottom sediments. All soil actinomycetes exhibited the highest activity in a culture medium containing colloidal chitin and shrimp shells. Actinomycetes collected in water and bottom sediments were characterized by similar activity levels. Chitinolytic fungi exhibited the maximum activity also in a medium containing colloidal chitin. The presence of shrimp shells in a culture medium had the lowest impact on chitinolytic activity. Among the analyzed forms of chitin, the impact of chitin powder on the activity of chitinases was the least stimulating.
8
A potent chitinolytic activity of Alternaria alternata isolated from Egyptian black sand
58%
Sharaf E. F.
Polish Journal of Microbiology
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2005
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tom 54
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nr 2
EN
Eight fungal species characterized by chitinolytic activity were isolated from Egyptian black sand collected from Rosetta coast. Genus Aspergillus and Alternaria alternata exhibited the highest density (> 40% of the total count, each) on the isolation plates containing different treatments of native shrimp shell chitin. Genus Aspergillus was represented by A. flavus, A. niger, A.foetidus and A. ungius, with the former species being the most dominant. The other species were Cladosporium herbarum, Fusarium equisitum (5.71% of the total count, each) and Dendryphiella vinosa (3.21% of the total count). The isolated species were screened for chitinase production on agar plates containing 0.2% colloidal chitin. The chitinolytic activity of each individual was not always correlated with its density on the isolation plates. Alternaria alternata was the most promising species for chitinase excretion. The use of colloidal chitin (1.5%) as a sole carbon source was superior for the enzyme production by A. alternata. Maximum enzyme yield was obtained after 7 days incubation at 30°C with shaking (150 rev min⁻¹), with an initial pH value of the growth medium at 5.0. Presence of NaN0₃ (0.3%), the best nitrogen source, and CaCl₂ (100 pg/ml) stimulated the induction of the enzyme. The crude A. alternata chitinase revealed a potential insecticidal effect on the larvae of fruitfly (82% mortality) and could degrade crude shrimp shell waste.
9
The in vitro antagonism between rhizobacterial and Fusarium strains
51%
Kurek E. , Jaroszuk J.
Acta Microbiologica Polonica
|
1997
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tom 46
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nr 1
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