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PL
W przedstawionej pracy oznaczano aktywność oksydazową ceruloplazminy oraz stężenia miedzi i cynku w surowicy krwi mężczyzn z przewlekłą miażdżycową niedrożnością tętnic (PMNT), tętniakiem aorty brzusznej (TAB) i w grupie kontrolnej. Stwierdzono wyższe stężenia miedzi i cynku oraz aktywność ceruloplazminy w surowicy w PMNT w porównaniu z TAB i kontrolą przy braku różnic między dwiema ostatnimi grupami. Aktywność oksydazowa ceruloplazminy we wszystkich badanych grupach jest dodatnio skorelawana z stężeniem miedzi (r≥0,64, 0≤0,05)., co wynika głównie z wiązania ponad 90 % miedzi przez to białko w osoczu krwi. W grupie PMNT aktywność ceruloplazminy jest także dodatnio skorelowana z stężeniem cynku. Należy sądzić, że wzrost aktywności oksydazowej ceruloplazminy oraz stężeń miedzi i cynku jest spowodowany reakcją ostrej fazy w związku z przewlekłym procesem zapalnym i martwiczym towarzyszącym niedrożności tętnic kończyn dolnych u części chorych.
EN
Oxidase activity of ceruloplasmin and concentrations of copper and zinc were determined in serum of men with atherosclerosis obliterans (AO), aortic abdominal aneurysm (AA) and control group. Higher concentrations of copper and zinc and activity of ceruloplasmin were found in AO in comparison with AA and control., and no significant differences were observed between AA and control. Oxidase activity of ceruloplasmin is positively correlated with concentration of copper in serum of all studied groups (r≥0.64, p≤0.05). It indicates a positive relationship between both ceruloplasmin and copper as more than 90 % of this element in plasma is bound to ceruloplasmin. Serum oxidase activity of ceruloplasmin is also positively correlated with zinc concentration in AO group. Increase in the activity of ceruloplasmin and concentrations of copper and zinc are observed probably due to inflamation and trauma accompanying chronic arterial occlusion of the lower limbs at least in a part of subjects in AO group.
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nr 08
997-1000
EN
The aim of the present study was to determine the cooperative interactions among some non-specific humoral factors participating in the immune response of healthy suckling piglets and piglets with diarrhea caused by E.coli at different ages. A total of 168 hybrid piglets (aged 12 to 18 days and 19 to 25 days), clinically healthy and suffering from diarrhea, were examined. The serum levels of lysozyme, ceruloplasmin (CP) and C-reactive protein (CRP) were determined in randomly selected sibs (clinically healthy piglets and piglets with diarrhea). Feces samples were taken from piglets with diarrhea to identify E. coli strains. A positive correlation between lysozyme activity and the serum concentration of CP was observed in healthy animals. This shows that over the experimental period the values of both these indices increased along parallel lines at a relatively stable CRP level. Elevated serum levels of lysozyme, CP and CRP in piglets with diarrhea caused by E. coli, as compared with healthy piglets, reflected the participation of these non-specific humoral factors in the immune response against E. coli. The increase in lysozyme activity, resulting from the immune response, was parallel to the increase in CP concentration (a positive correlation), but not parallel to the increase in CRP concentration (a negative correlation). These changes were found to be age-related. In younger piglets, the lysozyme activity was significantly correlated with CRP level but in older ones with level of CP.
EN
Copper is an essential metal in living organisms. They all require copper as a catalytic cofactor for biological processes such as respiration, iron transport, oxidative stress protection and pigmentation. Nevertheless, exposure to the excess of copper can damage cells and organs. Copper ions and complexes can induce formation of reactive oxygen species, that can damage biomolecules including unsaturated lipids and DNA. In the past few years exciting advances have been made toward understanding how copper is transported within cells. Dysfunction of copper metabolism can lead to its excess or defi-ciency. Two examples of illness related to alterations in copper metabolism are Menkes and Wilson diseases. The understanding of copper homeostasis has become important in clinical medicine as the metal could be involved in the pathogenesis of some neurological disorders such as Alzheimer, Parkinson or prion diseases.
EN
The aim of the study was to evaluate changes in enzymatic activity taking place in the organisms of fattening pigs, located in two different places; with closed and opened runs - with their breeding conditions in accordance with animal husbandry standards (on average: temperature - 21.1°C; 79.2% - humidity; velocity of air movement - 0.3 m/s²; cooling - mWxcm²). The animals were fed with identical mash mixtures ad libitum (with no limits). The increase of body mass and the daily average growth of both sexes of the examined cross-breads during their feeding period proceeded correctly. However, when the animals were located in two different places during the entire feeding period feeding, the most beneficial growth occurred in the animals held in places with opened runs (850 and 895 g). Changes in the activity of the examined enzymes in the blood serum of the animals were also found, especially during the final period of feeding. An increase in the maximum content of LDH enzyme took place in the blood serum of gilts and amounted to 55%. A similar tendency was found in hogs and amounted to 50%. Nevertheless, when this data was compared with the initial statistics the highest activity during the period of feeding was demonstrated by creatine kinase, since gilts had an average increase of that indicator of 112%, and hogs - 101%. The rapid change of the enzymatic profile of both LDH and CK in intensive feeding of fattening pigs has an adverse effect on their health.
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