Wyniki wyszukiwania - Biblioteka Nauki

1

Rsp5 ubiquitin ligase affects isoprenoid pathway and cell wall organization in S. cerevisiae.

100%

Kamińska J. , Kwapisz M. , Grabińska K. , Orłowski J. , Boguta M. , Palamarczyk G. , Żołądek T.

Acta Biochimica Polonica

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2005

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tom 52

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nr 1

207-220

EN

Dimethylallyl diphosphate, an isomer of isopentenyl diphosphate, is a common substrate of Mod5p, a tRNA modifying enzyme, and the farnesyl diphosphate synthase Erg20p, the key enzyme of the isoprenoid pathway. rsp5 mutants, defective in the Rsp5 ubiquitin-protein ligase, were isolated and characterized as altering the mitochondrial/cytosolic distribution of Mod5p. To understand better how competition for the substrate determines the regulation at the molecular level, we analyzed the effect of the rsp5-13 mutation on Erg20p expression. The level of Erg20p was three times lower in rsp5-13 compared to the wild type strain and this effect was dependent on active Mod5p. Northern blot analysis indicated a regulatory role of Rsp5p in ERG20 transcription. ERG20 expression was also impaired in pkc1Δ lacking a component of the cell wall integrity signaling pathway. Low expression of Erg20p in rsp5 cells was accompanied by low level of ergosterol, the main end product of the isoprenoid pathway. Additionally, rsp5 strains were resistant to nystatin, which binds to ergosterol present in the plasma membrane, and sensitive to calcofluor white, a drug destabilizing cell wall integrity by binding to chitin. Furthermore, the cell wall structure appeared abnormal in most rsp5-13 cells investigated by electron microscopy and chitin level in the cell wall was increased two-fold. These results indicate that Rsp5p affects the isoprenoid pathway which has important roles in ergosterol biosynthesis, protein glycosylation and transport and in this way may influence the composition of the plasma membrane and cell wall.

2

Characterization of cell wall pectins by vibrational spectroscopy

100%

Boeriu C. , Stolle-Smits T. , Van Dijk C.

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nr 2 Suppl.

3

Silencing of gene expression for secondary cell wall protein from Arabidopsis thaliana - functional analysis

100%

Derba M. , Jarmolowski A. , Wojtaszek P.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

4

Simple method to estimate cell wall extensibility coefficient by using only two cardinal numbers

88%

Pietruszka M.

Acta Societatis Botanicorum Poloniae

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2009

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tom 78

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nr 4

269-270

EN

In this short communication we consider the extensibility properties of the cell wall. This is accomplished by a heuristically motivated equation for the expanding volume of the cell. The experimentally determined characteristic time t0 and temperature T0 are the only numbers required for evaluating the effective yielding coefficient Ф(t, T) in the respective time and temperature domains.

5

Nanohardness and elasticity of cell walls of Scots pine (Pinus sylvestris L.) juvenile and mature wood

88%

Mania P. , Nowicki M.

Bulletin of the Polish Academy of Sciences. Technical Sciences

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2020

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tom Vol. 68, nr 5

1237--1241

EN

The aim of this study was to determine the hardness and reduced modulus of elasticity of juvenile wood of Scots pine (Pinus sylvestris L.) using the nanoindentation method, and then to compare the results obtained with those of mature wood. The hardness of juvenile pine wood determined by means of the nanoindentation method was 0.444 GPa while for mature wood it was 0.474 GPa. Statistically significant differences between the values were found. The reduced modulus of elasticity in juvenile wood was 14.0 GPa and 16.4 GPa in mature wood. Thus, the hardness values obtained were about 7% higher, while the modulus of elasticity was 17% higher in mature wood. All determinations were made in the S2-layer of the secondary cell wall.

6

Regeneration of the cell wall by isolated protoplasts of Solanum lycopersicoides Dun is a selective process

88%

Tylicki A. , Burza W. , Malepszy S. , Kuras M.

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tom 38

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nr 1

7

L-forms of Staphylococcus epidermidis indeced by penicillin

88%

Wroblewska J. , Janicka G. , Gospodarek E. , Szymankiewicz M.

Polish Journal of Microbiology

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2006

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tom 55

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nr 3

EN

L-forms of S. epidermidis were induced at 35°C with the use of an L-form medium with penicillin. The aim of this study was to evaluate the frequency of L-form induction and demonstrate whether the origin of the clinical strains affects the frequency of L-forms induction, as well as to study whether the time of action of the antibiotic has an influence on frequency of L-form induction.

8

Organismal view of a plant and a plant cell.

75%

Wojtaszek P.

Acta Biochimica Polonica

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2001

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tom 48

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nr 2

443-451

EN

Cell walls are at the basis of a structural, four-dimensional framework of plant form and growth time. Recent rapid progress of cell wall research has led to the situation where the old, long-lasting juxtaposition: "living" protoplast - "dead" cell wall, had to be dropped. Various attempts of re-interpretation cast, however, some doubts over the very nature of plant cell and the status of the walls within such a cell. Following a comparison of exocellular matrices of plants and animals, their position in relation to cells and organisms is analysed. A multitude of perspectives of the biological organisation of living beings is presented with particular attention paid to the cellular and organismal theories. Basic tenets and resulting corollaries of both theories are compared, and evolutionary and developmental implications are considered. Based on these data, "The Plant Body" - an organismal concept of plants and plant cells is described.

9

Wpływ obróbki cieplnej na strukturę wybranych warzyw i owoców ®

75%

Komolka P. , Górecka D.

Postępy Techniki Przetwórstwa Spożywczego

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2017

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tom Nr 2

67--73

PL

Podczas obróbki cieplnej warzyw i owoców zachodzą zróżnicowane zmiany w strukturze ich ściany komórkowej. Na podstawie przeprowadzonych badań własnych w artykule opisano zmiany w strukturze ściany komórkowej kapusty białej, czerwonej, włoskiej, marchwi, ziemniaków oraz jabłek, które zachodzą podczas ich obróbki cieplnej w wodzie, parze wodnej i podczas pieczenia. Polegają one na pogrubieniu ścian komórkowych, rozluźnieniu upakowania komórek, a tym samym powiększeniu przestrzeni międzykomórkowych.

EN

During the heat treatment of vegetables and fruits there are varied changes in the structure of their cell wall. This article describes changes in cell structure of white cabbage, red cabbage, savoy cabbage, carrots, potatoes and apples during boiling, steaming and baking. They consisted of thickening of cell walls, loosening of cellular packing, and thus enlargement of intercellular spaces.

10

Nanohardness and elasticity of cell walls of Scots pine (Pinus sylvestris L.) juvenile and mature wood

75%

Mania P. , Nowicki M. , Mania P. , Nowicki M.

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11

On some aspects of a relation between density and mechanical properties of wood in longitudinal direction

75%

Krauss A.

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nr 1

EN

A comprehensive analysis has been made of the mechanical properties and density of 100 timber species. The correlation between the mechanical properties and wood density has been approximated by a power function type y = aρn. No functional relation has been found between the parameters describing mechanical properties of the cell wall and the wood density. The values of these parameters show great scatter of about ±50% relative to the mean value. An attempt has been made to identify other wood characteristics determining the mechanical properties of wood. The species characterised by extremely high values of the parameters describing mechanical properties of wood have been singled out. The greatest differentiation in the values has been noted for the tensile strength. Among the coniferous species of similar density the differences have reached 113% on average, while among the deciduous species – 143%, at the differences in density being only of 15%.

PL

Analizowano niektóre właściwości mechaniczne i gęstość 100 gatunków drewna. Podjęto próbę wskazania na inne, poza gęstością, czynniki decydujące o właściwościach mechanicznych drewna. Związki między właściwościami mechanicznymi i gęstością drewna opisano funkcją potęgową typu y = aρn. Nie stwierdzono natomiast zależności funkcyjnych między właściwościami mechanicznymi ściany komórkowej i gęstością drewna. Zaobserwowano duży rozrzut wartości właściwości mechanicznych ścian komórkowych wynoszący do około ±50% w stosunku do wartości średniej. Wskazano na gatunki charakteryzujące się ekstremalnymi wartościami właściwości mechanicznych. Największe zróżnicowanie odnotowano w wytrzymałości drewna na rozciąganie podłużne, dla gatunków iglastych wynosiło ono średnio 113%, a dla gatunków liściastych – 143%, przy gęstości różniącej się odpowiednio o 5% i 15%.

12

Cell wall proteome of pathogenic fungi

75%

Karkowska-Kuleta J. , Kozik A.

Acta Biochimica Polonica

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2015

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tom 62

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nr 3

339-351

EN

A fast development of a wide variety of proteomic techniques supported by mass spectrometry coupled with high performance liquid chromatography has been observed in recent years. It significantly contributes to the progress in research on the cell wall, very important part of the cells of pathogenic fungi. This complicated structure composed of different polysaccharides, proteins, lipids and melanin, plays a key role in interactions with the host during infection. Changes in the set of the surface-exposed proteins under different environmental conditions provide an effective way for pathogens to respond, adapt and survive in the new niches of infection. This work summarizes the current state of knowledge on proteins, studied both qualitatively and quantitatively, and found within the cell wall of fungal pathogens for humans, including Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans and other medically important fungi. The described proteomic studies involved the isolation and fractionation of particular sets of proteins of interest with various techniques, often based on differences in their linkages to the polysaccharide scaffold. Furthermore, the proteinaceous contents of extracellular vesicles ("virulence bags") of C. albicans, C. neoformans, Histoplasma capsulatum and Paracoccidioides brasiliensis are compared, because their production can partially explain the problem of non-classical protein secretion by fungi. The role assigned to surface-exposed proteins in pathogenesis of fungal infections is enormously high, thus justifying the need for further investigation of cell wall proteomes.

13

Damage resistance and microstructure of barley kernels

75%

Fornal J. , Sadowska J. , Ornowski A. , Jelinski T. , Velikanov L.

International Agrophysics

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2000

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tom 14

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nr 2

14

Changes in water content, sugars and invertase activity in developing seeds of Hibiscus esculentum

75%

Thaker V.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 2

EN

Seeds of Hibiscus esculentum were analyzed for growth, in terms of fresh and dry weights, cell size, water content, reducing and non-reducing sugars and acid invertase activity. On the basis of growth analysis seed development is divided into four distinct phases of a) cell division, d) cell elongation, c) dry matter accumulation and, d) maturation. A close parallel with water content and cell size was observed. A peak level of reducing sugars was observed during the rapid elongation growth. The role of invertase in hydrolyzing sugars and its regulation of sink development is discussed.

15

Immunocytochemical localization of pectins in the maturing anther of Allium cepa L.

75%

Golaszewska B. , Bednarska E.

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nr 3

16

Turnover of cell wall components during internode growth in Merremia emarginata

75%

Patel D. , Thaker V. S.

Acta Physiologiae Plantarum

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2004

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tom 26

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nr 3

17

Cell wall - carotenoids of the alga Botrydium granulatum Visher [Botrydiaceae-Botrydiales]

75%

Kowalowski P. , Zych M. , Burczyk J. , Smietana B. , Terminska-Pabis K. , Stolarczyk A.

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nr 1

EN

Ściany komórkowe glonów Botrydium granulátum, szczep 11-1 (Botrydiaceae, Botrydiales) zwane są macierzystymi ścianami komórkowymi (CWM). Gromadzone są one w pożywce wzrostowej i wykazują intensywnie różowe zabarwienie. Podobne zabarwienie wykazują ściany komórkowe izolowane z homogenatu z rozdrobnionych komórek (CWH). Zabarwienie to jest spowodowane przez karotenoidy, takie jak echinenon, kantaksantyna, 3S,3’S-astaksantyna, ich produkty utlenienia, astacen i semiastacen, 3S,3’-fritschiellaksantyna, 2,3-didehydro-(6’R,3’R)-fritschiellaksantyna i (3R,3’R,6’R) - luteina. Udowodniono, że ściany komórkowe (CW) glonów należących do Botrydiaceae, z rzędu Botrydiales zawierają te same karotenoidy co karotenoidy izolowane z CW rzędu Chlorococcales. Ściany komórkowe glonów Botrydium granulátum, szczep 11-1 zawierają także biopolimer oporny na acetolizę.

PL

Cell walls of the alga Botrydium granulátum, strain 11-1 (Botrydiaceae, Botrydiales) so called maternal cell walls (CWM) which are liberated into the growth medium are intensively pinkish coloured. Similar colouration show cell walls isolated from the homogenate of disrupted algal cells (CWH). This pigmentation is caused by carotenoids identical with echinenone, canthaxanthin, 3S,3’S-astaxanthin, its oxidation products semiastacene and astacene, 3S,3’R-fritschiellaxanthin, 2,3-didehydro-(6’R,3’R) fritschiellaxanthin and (3R,3’R,6’R)-lutein. This paper give evidence, that cell walls (CW) of algae belonging Botrydiaceae, contains the same qualitatively carotenoid pattern as CW of Chlorococcales. Cell walls of the alga Botrydium granulátum strain 11-1 also contain an acetolysis resistant biopolymer (ARB).

18

Low temperature modifies cell wall mechanical properties and expansion growth of leaves

75%

Solecka D. , Zebrowski J. , Kacperska A.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

19

Pattern of pectin distribution in pollen tube cell wall

75%

Banas M.

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nr 1

20

Metabolism of [3-3H]oleanolic acid in the isolated Calendula officinalis leaf cells and transport of the synthesized glycosides to the cell wall and the extracellular space

75%

Szakiel A. , Wasiukiewicz I. , Janiszowska W.

Acta Biochimica Polonica

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1995

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tom 42

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nr 1

EN

It has been shown for the first time that [3-3H]oIeanolic acid glycosides formed in the cytosol of C. officinalis leaf cells are transported to the extracellular space in the form of pentaglucoside VI (44%), whereas glucuronides derived from [3-3H]oleanolic acid 3-O-monoglucuronide (29%) as well asa part of glucosides (24%) were transported into the cell walls.