Wyniki wyszukiwania - Biblioteka Nauki

1

Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures

100%

Mistrzak P. , Celejewska-Marciniak H. , Szypula W. J. , Olszowska O. , Kiss A. K.

Acta Societatis Botanicorum Poloniae

|

2015

|

tom 84

|

nr 1

EN

The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

2

High performance liquid chromatography based quantification of reserpine in Rauwolfia tetraphylla L. and enhanced production through precursor feeding

100%

Rohela G. K. , Bylla P. , Pendli S. , Korra R. , Gandu R. , Reuben C.

Acta Chromatographica

|

2022

|

tom Vol. 34, no. 2

120--129

EN

Rauwolfia tetraphylla L., is an important medicinal plant in Apocynaceae family and is recognized as an alternative source to Rauwolfia serpentina L., in terms of anti-hypertensive alkaloid production i.e. reserpine. In view of this, the present study is conducted to estimate the reserpine content in different parts (leaf, stem and root) of field grown plants (2 years old), tissue cultured plantlets (R1) (two months old) and cell suspensions cultures (two months old with and without precursor feeding) of R. tetraphylla by using high performance liquid chromatography (HPLC) technique. Overall maximum content of reserpine (in %) was estimated from the root samples. Roots of field grown plants has recorded high percent of reserpine (0.39%) followed by roots of tissue cultured plantlets (0.35%) and root callus based cell suspension cultures (0.38 %) which was fed with precursor amino acid (100 mg/L of tryptophan). In control type of root callus based cell suspension cultures, reserpine content was quantified as 0.14%; by precursor feeding (100 mg/L of tryptophan) it was enhanced to 0.38%. In conclusion, the reserpine content (0.35 and 0.38%) produced by the roots of tissue cultured plantlets (R1) and 100 mg/L tryptophan fed root callus based cell suspensions was comparable to that of the reserpine content (0.39%) of root parts of field grown plants. The present study demonstrates the reserpine production by in vitro cell suspension cultures throughout the year without sacrificing the medicinal plants.

3

Primary results of 2-dimensional electrophoresis for protein studies of Gentiana kurroo Royle somatic embryos derived from long-term embryogenic cell suspensions

100%

Niedziela A. , Rybczynski J. J.

Acta Societatis Botanicorum Poloniae

|

2014

|

tom 83

|

nr 3

EN

Two-dimensional gel electrophoresis was used to compare protein expression profiles between various stages of Gentiana kurroo Royle somatic embryos. Seven distinct stages (I–VII) were pointed out and measured from long-term embryogenic cell suspension. Isoelectric focusing was done in the pH intervals 3–10, and the second dimension was carried out with 13% SDS-polyacrylamide gel electrophoresis. Dependent on the stage from about 400 (stage IV) to more than 700 (stage II) protein spots were totally detected. The molecular weight of abundant proteins range from 12 to 70 kDa, however, majority of proteins were located between 20–49 kDa spots on the gels. The highest difference in the number of spots appeared in the case of globular embryo (stage I) and elongated cotyledonary stage (stage VII) with differences being about 130 spots. The relevance of embryogenic cell suspension choice for proteomic analysis as well as expediency of the increasing number of particular embryo stages is discussed.

4

Badania przydatności pompy wyporowej nowej konstrukcji do przetłaczania cieczy o strukturze wrażliwej na oddziaływania mechaniczne

100%

Lehmann S. , Jankowski T.

Inżynieria Rolnicza

|

2002

|

tom R. 6, nr 9

157--162

PL

W artykule przedstawiono badania pompy PWMD (pompa wyporowa o małej destrukcji) pod kątem jej przydatności do przetłaczania cieczy o strukturze wrażliwej na oddziaływania mechaniczne. Konstrukcja pompy powstała w oparciu o sformułowane wcześniej kryteria opracowane na podstawie literatury dotyczącej wpływu hydrodynamiki na zawiesiny biologiczne. Podstawę oceny stanowiły badania porównawcze z pompą krzywkową, a kryterium oceny wyniki badań Teologicznych zawiesiny komórek drożdży. Stwierdzono, że opracowana pompa działa mniej destruktywnie na tłoczone medium niż porównywana pompa krzywkowa.

EN

Paper presented a displacement pump of new construction, characterised by slight-in relation to other existing pumps - destructive influence on biological suspensions during their multiple flow through the assembly. The pump was designed according to earlier formulated criteria based on the literature concerning the effect of hydrodynamics on biological suspensions. The results of study on energy consumption showed that the pump reached higher efficiency during pumping liquids of viscosities higher than the water, at the pressure difference 0.15-0.22 MPa. Evaluation of the pump usability to forcing sensitive biological media was based on comparative tests with a lobar pump, where the yeast cell suspension was used as a material for rheological comparisons. The newly developed pump was found to affect less destructively the forced medium than the compared lobar pump.

5

Evaluation of intracellular Ca2plus concentration by Fura 2 ratiometry in encystment-induced Colpoda cucullus

100%

Sogame Y. , Matsuoka T.

Acta Protozoologica

|

2013

|

tom 52

|

nr 1

6

The modified method for isolation and culture of highly homogenous Leydig cell population from rat testes

100%

Janecki A. , Pawlowski T. , Lukaszyk A. , Kozak W.

Folia Histochemica et Cytobiologica

|

1984

|

tom 22

|

nr 3-4

7

Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (Cucumis sativus L.)

100%

Gzyl J. , Rymer K. , Gwóźdź E.

Acta Biochimica Polonica

|

2009

|

tom 56

|

nr 4

723-727

EN

Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.

8

Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

100%

Ryszawy D. , Podulka J. , Tworzydlo A. , Tomassy J. , Jarmulski M. , Madeja Z. , Korohoda W.

Folia Biologica

|

2019

|

tom 67

|

nr 4

9

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

100%

Maciejewska U. , Skierski J. S. , Szczerbakowa A.

Acta Physiologiae Plantarum

|

1999

|

tom 21

|

nr 1

EN

The DNA relative content in nuclei from several Solanum species, which were used as partners for somatic hybridization, were determined using a flow cytometry method. The nuclei were isolated mechanically or via protoplasts from leaves of in vitro grown plants. In the case of S. nigrum as well as S. tuberosum cv. Bzura and dihaploid clone H8105, the nuclei were also obtained from suspension cultured cells by lysis of protoplasts. The source and the method of nuclei isolation affected the pattern of nuclear DNA in the genotypes studied. The mesophyll nuclei showed two distinct peaks on the DNA histograms, whereas the DNA peaks produced by cell suspension nuclei were broad and less distinct. The DNA content in the nuclei, calculated from the DNA histograms of the samples and a DNA standard historgam (Trout Red Blood Cells, having DNA content of 5.05 pg per nucleus), were much lower in mesophyll nuclei than in those obtained from the cell suspension for the same genotypes. The results are discussed in respect of the genetic instability of Solanum genotypes. The usefulness of a flow cytometry approach in somatic hybridization research is also discussed.

10

Influence of some polyols on erythrocyte cytoskeleton proteins

100%

Nardid O. A. , Dyubko T. S. , Soloviova A. S. , Zinchenko A. V.

Cellular and Molecular Biology Letters

|

1998

|

tom 03

|

nr 2

11

Somatic embryogenesis of Gentiana genus. III. Characterization of three-year-old embryogenic suspensions of G.pannonica originated from various seedling explants

100%

Mikula A. , Skierski J. , Rybczynski J. J.

Acta Physiologiae Plantarum

|

2002

|

tom 24

|

nr 3

12

Differentiation of wheat cell suspension cultured in cold - impact of zearalenone and 24-epibrassinolide

84%

Janeczko A. , Biesaga-Koscielniak J. , Skoczowski A. , Marcinska I.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2007

|

tom 523

13

Cryopreserved tissue engineered mucosa

84%

Spoerl G. , Mai R. , Klingner E. , Gedrange T. , Lauer G.

|

tom 46

|

nr 4

541-544

14

Reversible and irreversible electroporation of cell suspensions flowing through a localized dc electric field

84%

Korohoda W. , Grys M. , Madeja Z.

Cellular and Molecular Biology Letters

|

2013

|

tom 18

|

nr 1

EN

Experiments on reversible and irreversible cell electroporation were carried out with an experimental setup based on a standard apparatus for horizontal electrophoresis, a syringe pump with regulated cell suspension flow velocity and a dcEF power supply. Cells in suspension flowing through an orifice in a barrier inserted into the electrophoresis apparatus were exposed to defined localized dcEFs in the range of 0–1000 V/cm for a selected duration in the range 10–1000 ms. This method permitted the determination of the viability of irreversibly electroperforated cells. It also showed that the uptake by reversibly electroperforated cells of fluorescent dyes (calcein, carboxyfluorescein, Alexa Fluor 488 Phalloidin), which otherwise do not penetrate cell membranes, was dependent upon the dcEF strength and duration in any given single electrical field exposure. The method yields reproducible results, makes it easy to load large volumes of cell suspensions with membrane non-penetrating substances, and permits the elimination of irreversibly electroporated cells of diameter greater than desired. The results concur with and elaborate on those in earlier reports on cell electroporation in commercially available electroporators. They proved once more that the observed cell perforation does not depend upon the thermal effects of the electric current upon cells. In addition, the method eliminates many of the limitations of commercial electroporators and disposable electroporation chambers. It permits the optimization of conditions in which reversible and irreversible electroporation are separated. Over 90% of reversibly electroporated cells remain viable after one short (less than 400 ms) exposure to the localized dcEF. Experiments were conducted with the AT-2 cancer prostate cell line, human skin fibroblasts and human red blood cells, but they could be run with suspensions of any cell type. It is postulated that the described method could be useful for many purposes in biotechnology and biomedicine and could help optimize conditions for in vivo use of both reversible and irreversible electroporation.

15

Improvement of growth parameters of prune callus cultures destined to initiate cell suspensions

84%

Hanus-Fajerska E.

Acta Societatis Botanicorum Poloniae

|

2006

|

tom 75

|

nr 1

5-9

EN

Callus was inducted on wounded leaf explants from shoot tips of a particular Prunus domestica 'Węgierka Zwykła' clone cultivated in vitro. The improvement of Sweet Common Prune stock callus tissue parameters has been approached by experiments on culture protocols. Either for the induction or maintenance of tissue modified Murashige and Skoog medium, supplemented with different auxins and cytokinins at varying concentrations, was used. The goal was to obtain the highiest possible proliferative capacity of friable tissue without any signs of cell redifferentiation for about 10 weeks. The choice of auxin was an important factor regulating the rate and kind of tissue growth, and for the examined prune clone auxin alone brought a relatively small proportion of cells into division, so advantageous was to combine it with oxygenated cytokinin. Friable tissue was obtained on media supplemented with dicamba or with picloram, but not with 2.4-D neither alone nor combinated with IBA.

16

Age related decline in functional T cell subsets inthe mouse

84%

Boersma W. J. A. , Vissinga C. S.

Folia Histochemica et Cytobiologica

|

1986

|

tom 24

|

nr 1

17

The effect of copper, zinc and iron ions on induction of the reactive oxygen species production in tobacco by-2 cell suspension culture

84%

Zrobek-Sokolnik A. , Gorecki R. J.

Polish Journal of Natural Sciences. Supplement

|

2003

|

tom 01

18

Studies on the superoxide anion production and peroxidase activity in potato leaf cell suspension exposed to salicylic acid

84%

Faravardeh L. , Rabbani A.

Acta Physiologiae Plantarum

|

2006

|

tom 28

|

nr 3

EN

It is now widely accepted that salicylic acid (SA) signaling is mediated by reactive oxygen species (ROS) production. We have studied the effect of SA on peroxidase activity and superoxide anion production in potato leaf cell suspension. The results show that potato cells are insensitive to low concentrations of exogenous SA (< 1 mM) and the effect is observed at 1-5 mM SA. The cells exposed to SA exhibit higher peroxidase activity and show different peroxidase pattern when antiyzed on native gels compared to the control. Superoxide anion production is enhanced after two hours of treatment and 2.5 mM SA gives the highest value. The results suggest peroxidase-mediated detoxification of ROS elicited by SA.

19

The use of TTC reduction assay for assessment of Gentiana spp. cell suspension viability after cryopreservation

84%

Mikula A. , Niedzielski M. , Rybczynski J. J.

Acta Physiologiae Plantarum

|

2006

|

tom 28

|

nr 4

EN

This study was aimed at improving the 2,3,5-triphenyltetrazoliumchloride (TTC) reduction test for initial assessment of cell survival after cryopreservation. Experiments were carried out on three embryogenic cell suspensions of different ages: 9-year-old Gentiana tibetica (King ex Hook. F.), 2-year-old G. kurroo (Royle), and 1-year-old G. cruciata (L.). The suspensions were maintained in MS medium supplef mented with 1.0 mgl-1 3,6-dichloro-o-anisic acid, 0.1 mgl-1 naphthaleneacetic acid, 2.0 mgl-1 6-benzylaminopurine, 80.0 mg l-1 adenine sulphate and 0.09 M sucrose. Four weeks before freezing, part of the tissue was subcultured to the same medium with sucrose concentrations elevated from 0.09 M (3%sMS) to 0.175 M (6%sMS) or 0.26 M (9%sMS). In freezing treatments without cryoprotection, tissue was plunged directly into liquid nitrogen (LN) or cooled gradually. In freezing treatments with cryoprotection, the cells were pretreated with 1 M sucrose, or with 0.4 M sorbitol + 0.25 M pro line or + 0.08 M DMSO, or with vitrification solufion (PVS2). Encapsulation was another variant. TTC reduction activity was spectrophotometrically assessed immediately, 1,3,5,24 and 48 h after thawing. Cells without cryoprotection were lethally damaged, but TTC reduction activity in those cells ranged from 6.5 % (tissue from 3%sMS) to 73 % (tissue from 9%sMS) directly after thawing. Formazan production was reduced to zero afier 24 h. The TTC test showed 50 % formazan content immediately after thawing of DMSO-protected G. tibetica tissue, but only 22.47 % after 24 h and 2.9 % afier 48 h. Ultrastructural analysis of those cells showed lethal damage in many of them. For the PVS2 treatment, the formazan confent was similar in samples analyzed directly after thawing and 24 h later. Cells treated with PVS2 did not show structural disturbances. Encapsulated cell aggregates of G. cruciata treated with concentrations of sucrose increasing up to 1 M produced 2.6 times more formazan. When applied at least 48 h after thawing, the TTC test can reflect cell viability and can be used to compare the effectiveness of cryoprotectant performance and freezing protocols, but it must be carefully evaluated, with appropriate controls.

20

Effects of cell suspension and cell-free culture filtrate of Pseudomonas aeruginosa in the control of root rot-root knot disease complex of tomato [Lycopersicon esculentum Mill.]

84%

Siddiqui I. A.

Acta Agrobotanica

|

2000

|

tom 53

|

nr 2

EN

The plant growth-promoting rhizobacterium Pseudomonas aeruginosa strain IE-6 was tested for antagonistic activity towards Meloidogyne javanica, the root-knot nematode and soilbome root-infecting fungi viz., Macrophomina phaseolina. Fusarium solani and Rhizoctonia solani under laboratory and greenhouse conditions. Cell-free culture filtrate of the bacterium caused significant reduction in egg hatching of M. javanica and inhibited radial growth of fungi in vitro. Cell-free culture filtrate also caused lyses in mycelium of F. solani. Under greenhouse conditions, soil drenches with the aqueous celi suspension or cell-free culture resulted in a considerable reduction in nematode population densities in soil and subsequent root-knot development due to M.javanica. In addition to nematode control, rhizobacterium application also inhibited root-infection caused by soilborne root-infecting fungi with significant enhancement of growth of tomato seedlings.

PL

W warunkach laboratoryjnych i szklarniowych była testowana bakteria ryzosferowa Pseudomonas aeruginosa szczep IE-6 oddziaływująca korzystnie na wzrost roślin, dla określenia antagonistycznej aktywności względem Meloidogyne javanica nicienia powodującego guzowatość korzeni oraz takich grzybów glebowych porażających korzenie jak Macrophomina phaseolina, Fusarium solani i Rhizoctonia solani. Filtraty kultur bez komórek bakterii powodowały znaczną redukcję wylęgania się z jaj larw Meloidogyne javanica i hamowały promienisty wzrost grzybów w warunkach in vitro. Filtraty kultur bez komórek powodowały także lizę strzępek F. solani. W warunkach szklarniowych zanotowano znaczną redukcję gęstości populacji nicieni w glebie, a następnie rozwoju guzów na korzeniach powodowanych przez M. javanica, gdy do gleby wprowadzono wodną zawiesiną komórek lub filtrat bez komórek. Zwalczanie nicieni oraz zastosowanie bakterii ryzosferowej ograniczało porażenie korzeni także przez grzyby przeżywające w glebie oraz wspomagało wzrost siewek pomidora.