Wyniki wyszukiwania - Biblioteka Nauki

1

Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells.

100%

Gołębiewska J. , Rozwadowski P. , Spodnik J. H. , Knap N. , Wakabayashi T. , Woźniak M.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

59-65

EN

We have demonstrated for the first time that the steroid metabolite, 2-methoxyestradiol (2-ME) is a powerful growth inhibitor of human osteosarcoma 143 B cell line by pleiotropic mechanisms involving cell cycle arrest at two different points and apoptosis. The ability of 2-ME to inhibit cell cycle at the respective points has been found concentration dependent. 1 μM 2-ME inhibited cell cycle at G1 phase while 10 μM 2-ME caused G2/M cell cycle arrest. As a natural estrogen metabolite 2-ME is expected to perturb the stability of microtubules (MT) in vivo analogously to Taxol - the MT binding anticancer agent. Contrary to 2-ME, Taxol induced accumulation of osteosarcoma cells in G2/M phase of cell cycle only. The presented data strongly suggest two different mechanisms of cytotoxic action of 2-ME at the level of a single cell.

2

Mechanisms of DNA damage and repair, studied during the cell cycle

100%

Rzeszowska J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

3

CAMPTOTHECIN INHIBITS MIGRATION, INVASION AND CLONOGENIC PROPERTY OF LIVER CANCER CELLS BY MODULATING MICRORNA EXPRESSION

88%

Wu S. , Li X. , Liu Z.

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tom 77

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nr 2

295-304

EN

Camptothecin (CPT), an alkaloid natural product, extracted from Camptotheca acuminata bark, has been reported to have potential antitumor activity in diverse cancers. MicroRNAs (MiRNAs) are a class of short, non-coding RNAs that plays a crucial role in the normal physiology by attenuating translation. Here, we showed that the CPT modulates the expression of miRNAs in hepatocellular carcinoma cells (HCC). Microarray analysis reveals that CPT modulates the expression of as many as 39 miRNAs in HCC cells (Huh7), 27 miRNAs were downregulated whereas 12 miRNAs were upregulated. miR-16 is the key miRNA upregulated by CPT and targets key prosurvival proteins (MMP-2, MMP-9 and cyclin D1). Our results demonstrate that CPT is inhibiting cell viability of HCC cells significantly when compared with the untreated cells. Wound healing and colony formation assay confirm inhibition of cell migration and clonogenic property of Huh7 cells respectively, upon the dose-dependent treatment of CPT. Furthermore, the Boyden chamber assay analysis revealed a significant inhibition of number of invasive cells in CPT treated cells with comparison to untreated Huh7 cells. Mechanistically, CPT upregulates miR-16 expression which targets MMP-2, MMP-9, cyclin D1 downregulation and subsequently upregulates the expression of E-cadherin, TIMP1, p21, and p27, thereby inhibits cell migration, invasion and clonogenic property of HCC cells. In summary, CPT treatment in Huh7 cells decreases cell viability and upregulates miR-16 expression, which results in inhibition of cell migration, invasion and clonogenic property of cells, by decreasing MMP-2, MMP-9, cyclin D1 and increasing the expression of cell cycle-regulated proteins p21 and p27.

4

The influence of cAMP and calcium on the modulation of the MP and ion conductivity in developing embryos

88%

Chaban V. , Goida E.

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1994

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tom 12

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nr 2

5

Genes regulating the cell cycle, mitosis and meiosis

88%

Rogalska S. M.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

6

Structural studies on CDK2-inhibitor complexes

88%

Endicott J.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

7

Influence of G2 arrest on the cytotoxicity of DNA topoisomerase inhibitors toward human carcinoma cells with different p53 status

88%

Bozko P. , Larsen A. K. , Raymond E. , Skladanowski A.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

EN

We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-incubation in drug-free medium. RKO cells expressing wild-type, functional p53 arrested the cell cycle progression in both the Gj and G2 phases of the cell cycle whereas the RKO/E6 cells, which lack functional p53, only arrested in the G2 phase. Growth-arrested cells did not resume proliferation even after prolonged incubation in drug-free medium (up to 96 h). To evaluate the importance of the cell cycle arrest on cellular survival, a non-toxic dose of UCN-01 (100 nM) was added to the growth-arrested cells. The addition of UCN-01 was accompanied by mitotic entry as revealed by the appearance of condensed chromatin and the MPM-2 phosphoepitope, which is characteristic for mi- totic cells. G2 exit and mitotic transit was accompanied by a rapid activation of caspase-3 and apoptotic cell death. The influence of UCN-01 on the long-term cytotoxic effects of the two drugs was also determined. Unexpectedly, abrogation of the G2 arrest had no influence on the overall cytotoxicity of either drug. In contrast, addition of UCN-01 to cisplatin-treated RKO and RKO/E6 cells greatly increased the cytotoxic effects of the alkylating agent. These results strongly suggest that even prolonged cell cycle arrest in the G2 phase of the cell cycle is not necessarily coupled to efficient DNA repair and enhanced cellular survival as generally believed.

8

A characterization of the temperature-sensitivity of an ovarian carcinoma cell line [OvBH-1], independent of p53 status

88%

Bar J. K. , Harlozinska A. , Wyrodek W. , Kartarius S. , Montenarh M. , Rodriguez-Parkitna J. M. , Kochman M. , Ozyhar A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

9

DNA replication reaction in Xenopus cell-free system is suppressed by high pressure

88%

Takahashi H. , Yamaguchi T. , Koga M. , Kageura H. , Terada S.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 3

EN

Previously, we demonstrated that when mouse erythroleukemia cells are exposed to a pressure of 80 MPa, the cell-cycle progression of S-phase cells is retarded. To examine the effects of high pressure on DNA replication, we used a Xenopus cell-free system. From cell-cycle progression of sperm nuclei, it was found that sperm nuclei are stable to a pressure of 80 MPa, whereas egg extracts are susceptible to high pressure. Similarly, biotin-16-dUTP was incorporated into 80 MPa-treated sperm nuclei in pressure-untreated extracts, but not into naive sperm nuclei in 80 MPa-treated extracts. These results indicate that DNA replication in Xenopus cell-free system is suppressed by the susceptibility of the extracts to a pressure of 80 MPa.

10

Human SUV3 helicase regulates growth rate of the HeLa cells and can localize in the nucleoli

75%

Szewczyk M. , Fedoryszak-Kuśka N. , Tkaczuk K. , Dobrucki J. , Waligórska A. , Stępień P.

Acta Biochimica Polonica

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2017

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tom 64

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nr 1

177-181

EN

The human SUV3 helicase (SUV3, hSUV3, SUPV3L1) is a DNA/RNA unwinding enzyme belonging to the class of DexH-box helicases. It localizes predominantly in the mitochondria, where it forms an RNA-degrading complex called mitochondrial degradosome with exonuclease PNP (polynucleotide phosphorylase). Association of this complex with the polyA polymerase can modulate mitochondrial polyA tails. Silencing of the SUV3 gene was shown to inhibit the cell cycle and to induce apoptosis in human cell lines. However, since small amounts of the SUV3 helicase were found in the cell nuclei, it was not clear whether the observed phenotypes of SUV3 depletion were of mitochondrial or nuclear origin. In order to answer this question we have designed gene constructs able to inhibit the SUV3 activity exclusively in the cell nuclei. The results indicate that the observed growth rate impairment upon SUV3 depletion is due to its nuclear function(s). Unexpectedly, overexpression of the nuclear-targeted wild-type copies of the SUV3 gene resulted in a higher growth rate. In addition, we demonstrate that the SUV3 helicase can be found in the HeLa cell nucleoli, but it is not detectable in the DNA-repair foci. Our results indicate that the nucleolar-associated human SUV3 protein is an important factor in regulation of the cell cycle.

11

Rapid proliferation of activated lymph node CD4plus T cells is achieved by greatly curtailing the duration of gap phases in cell cycle progression

75%

Mishima T. , Toda S. , Ando Y. , Matsunaga T. , Inobe M.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 4

EN

Peripheral T cells are in G0 phase and do not proliferate. When they encounter an antigen, they enter the cell cycle and proliferate in order to initiate an active immune response. Here, we have determined the first two cell cycle times of a leading population of CD4+ T cells stimulated by PMA plus ionomycin in vitro. The first cell cycle began around 10 h after stimulation and took approximately 16 h. Surprisingly, the second cell cycle was extremely rapid and required only 6 h. T cells might have a unique regulatory mechanism to compensate for the shortage of the gap phases in cell cycle progression. This unique feature might be a basis for a quick immune response against pathogens, as it maximizes the rate of proliferation.

12

Tobacco smoking alters the number of oral epithelial cells with apoptotic features

75%

Michcik A. , Cichorek M. , Daca A. , Chomik P. , Wojcik S. , Zawrocki A. , Wlodarkiewicz A.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 1

13

3-Fluoromethcathinone, a structural analog of mephedrone, inhibits growth and induces cell cycle arrest in HT22 mouse hippocampal cells

75%

Siedlecka-Kroplewska K. , Szczerba A. , Lipinska A. , Slebioda T. , Kmiec Z.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 2

14

The use of quantitative methods of cytochemical studies covering the nuclear chromatin in the determination of cell cycle phases. I. Feulgen method

75%

Kowalska E.

Folia Morphologica

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1986

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tom 45

|

nr 1

15

LaCl3 Induces Genomic DNA Instability and Increases DNA Methylation Levels in Wheat Roots

75%

Lei X. , Ma K. , Zhang F. , Lei X. , Ma K. , Zhang F.

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16

The use of quantitative methods of cytochemical studies covering the nuclear chromatin in the determination of cell cycle phases. II. Method of autoradiography

75%

Kowalska E.

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nr 2

17

Protein phosphatase 2A: Variety of forms and diversity of functions

75%

Lechward K. , Awotunde O. S. , Świątek W. , Muszyńska G.

Acta Biochimica Polonica

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2001

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tom 48

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nr 4

921-933

EN

Protein phosphatase 2A (PP2A) comprises a diverse family of phosphoserine-and phosphothreonine-specific phosphatases present in all eukaryotic cells. All forms of PP2A contain a catalytic subunit (PP2Ac) which forms a stable complex with the structural subunit PR65/A. The heterodimer PP2Ac-PR65/A associates with regulatory proteins, termed variable subunits, in order to form trimeric holoenzymes attributed with distinct substrate specificity and targeted to different subcellular compartments. PP2Ac activity can be modulated by reversible phosphorylation on Tyr307 and methylation on C-terminal Leu309. Studies on PP2A have shown that this enzyme may be implicated in the regulation of metabolism, transcription, RNA splicing, translation, differentiation, cell cycle, oncogenic transformation and signal transduction.

18

Application of NucleoCounter for the comprehensive assessment of murine cultured neurons during infection with Equine Herpesvirus type 1 (EHV-1)

75%

Chodkowski M. , Serafińska I. , Brzezicka J. , Bańbura M. W. , Cymerys J.

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nr 4

19

LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots

75%

Lei X. , Ma K. , Zhang F.

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2021

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tom 63

|

nr 1

20

Protein and siRNA delivery by transportan and transportan 10 into colorectal cancer cell lines

75%

Wierzbicki P. M. , Kogut-Wierzbicka M. , Ruczynski J. , Siedlecka-Kroplewska K. , Kaszubowska L. , Rybarczyk A. , Alenowicz M. , Rekowski P. , Kmiec Z.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 4