Wyniki wyszukiwania - Biblioteka Nauki

1

Insilico Studies of activities of compounds from Ricinodendron heudelotii against inflammatory and oxidant enzymes Carbonic anhydrase

100%

Ikezu U. J. M. , Ugariogu S. N.

World News of Natural Sciences

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2024

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tom 55

119-153

EN

Medicinal plants have been acclaimed and documented over the years to play vital role in promoting human health. The study evaluated the activities of the compounds extracted from the Ricinodendron heudelotii seed against carbonic anhydrase enzyme which is responsible for inflammation and oxidation in the body. In this study, phytocompounds from the seed were extracted and characterized using gas chromatography coupled to a mass selective detector to identify the component phytochemicals responsible for its anti-oxidation and inflammatory activity. Site directed multi-ligand docking of the identified compounds was performance on Crystal structure of human Carbonic anhydrase I in complex with polmacoxib as the cocrystalline ligand with (PDB ID:5gmm). The compounds identified from GCMS results were compared with some standard anti-inflammatory and antioxidant drugs like Diclofenac, Indomethacin, Celecoxib, Naproxen and the cocrystaline ligand Polmacoxib. The docking result showed that the cocrystallized ligand have the best binding affinity of -8.5 kcal/mol followed by Naproxen (drug) -7.6 kcal/mol, phytocompound 1,2-Benzisothiazole, 3-(hexahydro-1H-azepin-1-yl)-, 1,1-dioxide and a commercial drug Celecoxib has the same affinity -7.5 kcal/mol, better than Diclofenac -7.0 kcal/mol, Indomethacin (drug) -6.7 kcal/mol, other phytocompounds like Phenol, 2-methoxy-4-(2-propenyl)-, acetate -6.4 kcal/mol, 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester -6.1 kcal/mol also showed good binding affinities with the protein showing that most of the compounds may have good anti-inflammatory and antioxidant properties there by validating the ethnomedical claims of the plant use as having anti-inflammatory potentials. The interactions of the phytocompounds with better binding affinity were visualized and their results proved that the dockings were done in the active sites with strong bonds. ADMET properties of the drugs, cocrystalline ligand and compounds with good binding affinities were carried out to check for their adsorption, distribution, metabolism, excretion and toxicity properties. The result showed that the compounds and drugs have good absorption, distribution, metabolism with the human body and are not toxic to the body. The research justifies the local claims on the use of the plant and strengthens the relevance of these compounds as promising lead candidates for the treatment of anti-inflammatory diseases.

2

The toxic Doppelganger: on the ionic and molecular mimicry of cadmium

100%

Chmielowska-Bąk J. , Izbiańska K. , Deckert J.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

369-374

EN

Cadmium is a toxic heavy metal which can cause numerous alterations in cell functioning. Exposure to cadmium leads to generation of reactive oxygen species, disorders in membrane structure and functioning, inhibition of respiration, disturbances in ion homeostasis, perturbations in cell division, and initiation of apoptosis and necrosis. This heavy metal is considered a carcinogen by the Agency for Toxic Substances and Disease Registry. At least some of the described toxic effects could result from the ability of cadmium to mimic other divalent ions and alert signal transduction networks. This review describes the role of cadmium mimicry in its uptake, reactive oxygen species generation, alterations in calmodulin, Wnt/β-catenin and estrogen signaling pathways, and modulation of neurotransmission. The last section is dedicated to the single known case of a favorable function performed by cadmium mimicry: marine diatoms, which live in zinc deficient conditions, utilize cadmium as a cofactor in carbonic anhydrase - so far the only described cadmium enzyme.

3

Carbonic anhydrase production by Pseudomonas fragi

75%

Tietze T. , Jaworska M. M. J.

Chemical and Process Engineering : New Frontiers

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2023

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tom Vol. 44, nr 1

art. no. e6

EN

Carbonic anhydrase is an important enzyme that can play a significant role in the processes of lowering carbon dioxide concentration in the atmosphere. The aim of the work was to investigate the extracellular carbonic anhydrase (CA) production by the bacteria Pseudomonas fragi. In the research, we focused on the evaluation of the phase of bacterial growth correlated with carbonic anhydrase production and on the evaluation of induction of CA production by calcium carbonate concentration in the nutrient medium. Presented data indicated that calcium carbonate can serve as the only carbon source for Pseudomonas fragi, inducing carbonic anhydrase secretion to culture broth. The enzyme was produced mainly in the adaptation growth phase reaching the maximal activity at the end of this phase or at the beginning of the growth phase. The maximal enzyme activity detected in all batches was at a similar level. The enzyme activity was constant but lower in the exponential phase growth. Therefore, the enzyme production is not growth-dependent, but it is correlated with bacteria adaptation to cultivation conditions.

4

Potent antifungal properties of gallic acid in Sarcochlamys pulcherrima against Candida auris

75%

Akhtar N. , Pandey D. , Mannan A. U. , Sarkar A. , Sharma H. , Kumar M. , Ghosh A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2023

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tom 104

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nr 2

5

Pharmacological impact on loop gain properties to prevent irregular breathing

75%

Kiwull-Schone H. , Teppema L. , Wiemann M. , Kalhoff H. , Kiwull P.

Journal of Physiology and Pharmacology

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2008

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tom 59

|

nr 1

EN

Theory predicts respiratory instabilities at elevated system loop gain (G), determined by such factors as ventilatory CO2 sensitivity, set-point PCO2, and metabolic rate. In anesthetized rabbits, the effects on G of carbonic anhydrase (CA) inhibitors and of different sodium/proton exchanger type 3 (NHE3) inhibitors were studied. Acetazolamide significantly reduced G by 42.0 ± 9.3% and methazolamide by 35.0 ± 9.5% (each n = 7, P<0.01). Irrespective of the substance, NHE3 inhibition reduced G by 33.0 ± 7.8% (n = 10, P<0.01) at 35.5 ± 1.6 mmHg PaCO2 (mean ±SE), but not at lower arterial CO2 levels (n=5). Since high baseline PCO2 coincides with elevated brainstem NHE3 mRNA expression, this may also account for a higher risk of sleep apnea (or even occurrence of sudden infant death). Therefore, NHE3 inhibitors may gain similar therapeutic importance in the treatment of irregular breathing as CA inhibitors. Generally, effective treatment should aim at a low system loop gain, by reducing respiratory chemosensitivity, improving blood gases and preventing low metabolic rates.

6

The toxic Doppelganger: on the ionic and molecular mimicry of cadmium

63%

Chmielowska-Bak J. , Izbianska K. , Deckert J.

Acta Biochimica Polonica

|

2013

|

tom 60

|

nr 3

EN

Cadmium is a toxic heavy metal which can cause numerous alterations in cell functioning. Exposure to cadmium leads to generation of reactive oxygen species, disorders in membrane structure and functioning, inhibition of respiration, disturbances in ion homeostasis, perturbations in cell division, and initiation of apoptosis and necrosis. This heavy metal is considered a carcinogen by the Agency for Toxic Substances and Disease Registry. At least some of the described toxic effects could result from the ability of cadmium to mimic other divalent ions and alert signal transduction networks. This review describes the role of cadmium mimicry in its uptake, reactive oxygen species generation, alterations in calmodulin, Wnt/β-catenin and estrogen signaling pathways, and modulation of neurotransmission. The last section is dedicated to the single known case of a favorable function performed by cadmium mimicry: marine diatoms, which live in zinc deficient conditions, utilize cadmium as a cofactor in carbonic anhydrase - so far the only described cadmium enzyme.

7

Comparative analysis of alphaglobin and carbonic anhydrase I cDNAs of the opossum Monodelphis domestica with related vertebrate sequences

63%

Barome P. O.

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tom 51

|

nr 1-2

8

Carboxylating enzymes and carbonic anhydrase functions were suppressed by zinc deficiency in maize and chickpea plants

51%

Salama Z. A. , El-Fouly M. M. , Lazova G. , Popova L. P.

Acta Physiologiae Plantarum

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2006

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tom 28

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nr 5

EN

Maize and chickpea plants were grown in a controlled environment with 0.5 pM Zn or without Zn and various photosynthetic reactions were studied. The chlorophyll level, the rate of photosynthesis and photosystem II activity, the activity of carboxylating enzymes and that of carbonic anhydrase were suppressed by Zn deficiency in both plant species. Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was quantified using polyacrylamide gel electrophoresis. Growing plants in a medium without Zn caused a decrease in the total protein level and in the levels of large and small subunits of Rubisco.

9

Oszacowanie dystansu genetycznego u owiec na podstawie polimorfizmu transferyn, hemoglobiny i anhydrazy węglanowej

44%

Bojczuk H. , Bojczuk B. , Zurkowski M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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1988

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tom 352

RU

На основании анализа трех полиформных систем белков ( Hb ,Tf и CA), рассматривая частоту аллелей, коэффициент гомозиготности, коэффициент „чистопородности", коэффициент сродственности и генетическую дистанцию, можно говорить о существенных генетических различиях между сравниваемыми четырьмя популяциями овец: мериносом, низинной овцой и овцой разновидности Желязна.

EN

The analysis of three polymorphic arrangements of proteins (Hb, Tf and CA) and consideration of the frequency of alleles, heterozygocity coefficient, "breed purity" coefficient, relation coefficient and genetic distance proved 1tie existence of significant genetic differences between the four sheep populations compared, viz.: Merino, Lowland, Wielkopolska and Żelazna sheep.