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In vitro morphogenic events in culture of Lotus corniculatus L. seedling root explants

100%

Rybczynski J. J. , Karolkowska M. , Kaczmarek Z. , Mikula A. , Fiuk A.

nr 3

191-200

The experiments were carried out on Lotus corniculatus (L.) seedling root explants of the cultivar varieties Skrzeszowicka, Caroll A10 and strain 175. Callus formation and shoot regeneration were the major explant response depended mainly on of the studied genotype and used plant growth regulators (PGRs). Primary cortex of proximal and distal end of explant was the most active tissue for callus proliferation. For shoot primordia differentiation deeper zones of cortex took a part. The process of meristematic centre initiation was not uniform and various level of shoot differentiation events were observed not earlier than 3 weeks of culture. Usually, the shoot primordia regeneration began on proximal rather than distal end of the explant. BAP rather than urea derivatives stimulated shoot proliferation in extended cultures. Increasing of BAP and TDZ concentrations brought about the explant polarity and expansion of the meristematic zones. The explant position in root did not have significant influence on the number of regenerated shoots. The cultures only had better bud formation by TDZ when compared to BAP. BAP stimulated bud formation and development of the shoots from them. Short term of TDZ treatment of explants stimulated meristem formation which developed into buds and shoots. CPPU stimulated callus proliferation and bud formation when explants pretreatment was prolonged from 12 to 36 hrs.

Somatic embryogenesis from broccoli stigmas in tissue culture

100%

Zenkteler M. , Zenkteler E. , Dostatnia I.

tom 48

nr 2

The factors regulating callus proliferation and bud regeneration from stigma tissues are not sufficiently understood. To study the regenerative capacity of pistil elements, pistil of broccoli was cultured under simple culture conditions. Stigmas with style from broccoli pistils undergo somatic embryogenesis on Murashige and Skoog basal medium. Callus initiation occurred on basal medium supplemented with 4 mg·l-1 BAP, 1.6 mg·l-1 2,4-D, 250 mg·l-1 casein hydrolysate and 30 g·l-1 sucrose. Proembryo induction was observed after two callus subcultures. Calluses with globular embryos were cultured on basal medium with 2 mg·l-1 BAP, 1 mg·l-1 IAA and 40 g·l-1 sucrose for development, maturation and germination of somatic embryos. A population of somatic embryos was maintained on medium containing 1 mg·l-1 BAP and 2 mg·l-1 NAA only. Adding NAA to MS medium containing BAP considerably enhanced root formation. After acclimatization, all plantlets developed well and produced phenotypically normal flowers.