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EN
The effects of Cd, Ni, Cu, or Zn on the whole cell-derived fatty acid profiles of four bacterial strains isolated from heavy metal-polluted soils located in Upper Silesia was determined. Based on the fatty acid methyl ester (FAME) profiles, the strains were identified and named as Enterobacter intermedius AM15, Enterobacter intermedius MH8b, Pseudomonas putida MH1d, and Klebsiella pneumoniae AM12. The obtained results showed changes that were dependent both on tested strains and metal used. The most significant changes were observed for strains cultured in the Ni presence. In the FAME profiles of MH8b, AM15, and AM12 strains, a significant increase of cyclopropane fatty acids was observed. Moreover, exposure for Ni resulted in the appearance of a new fatty acid in the FAME profiles of AM15 and MH8b strains. In turn, Cd and Zn caused a decrease of the content of cyclopropane fatty acids as compared to control. For AM15 and AM12 strains cultured on media with heavy metals, the ratio of saturated to unsaturated fatty acids were higher than that in control. The same phenomenon was also observed for MH8b strain exposed only to the highest concentration of Ni and Cd.
EN
The influence of Cd concentration in growing substrate (0, 10, 20, 40 mg·dm⁻³) and AMF colonization of root system on growth, flowering, and Cd accumulation in osteospermum shoots were examined. Cd in the applied concentrations did not affect decorative value of osteospermum, no chlorosis or necrosis were observed on leaf margins frequently visible on leaves of other bedding plants. Cd at higher concentrations (20 and 40 mg·dm⁻³) lowered dry weights of shoots and roots in non-mycorrhizal plants. Mycorrhizal plants were lower and had lower dry weights of shoots and roots. Cd did not affect dry weights of shoots and roots in mycorrhizal plants. The effects of Cd and mycorrhization on flowering of osteospermum were negligible. Cd content of osteospermum shoots increased with the increasing content of Cd in growing substrate. Mycorrhizal plants grown in substrate containing 40 mg Cd·dm⁻³ accumulated more Cd in shoots than non-mycorrhizal plants. Obtained results showed that osteospermum is very tolerant to Cd toxicity and can accumulate great amount of Cd in shoots.
PL
Badania miały na celu określenie wpływu zawartości kadmu w podłożu (0, 10, 20, 40 mg·dm⁻³) i mikoryzy arbuskulamej na wzrost, kwitnienie i akumulację Cd w pędach osteospermum. Cd w zastosowanych stężeniach nie obniżał wartości dekoracyjnej osteospermum, nie powodował chlorozy i nekroz na brzegach liści, wyraźnie widocznych u innych roślin rabatowych. Cd w wyższych stężeniach (20 i 40 mg·dm⁻³) powodował spadek suchej masy części nadziemnych i korzeni roślin niezmikoryzowanych. Rośliny zmikoryzowane były niższe i miały mniejszą suchą masę niż niezmikoryzowane. Cd nie obniżał suchej masy pędów i korzeni roślin zmikoryzowanych. Wpływ Cd i mikoryzacji na kwitnienie osteospermum był nieznaczny. Zawartości Cd w pędach osteospermum wzrastały wraz ze wzrostem zawartości tego pierwiastka w podłożu. Rośliny zmikoryzowane rosnące w podłożu zawierającym 40 mg Cd·dm⁻³ akumulowały znacznie więcej Cd w pędach niż rośliny niezmikoryzowane. Otrzymane wyniki wskazują, że osteospermum można zaliczyć do roślin bardzo tolerancyjnych w stosunku do kadmu i zdolnych do akumulacji znacznych ilości tego pierwiastka w pędach.
EN
To investigate the biological activities of anthocyanins, which are induced by cadmium in A. imbricata, the antioxidant properties of anthocyanins were investigated using various antioxidant assays, namely 1,1 -diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azinobis-(3-ethylbenzthiazoline-6- sulfonic acid) (ABTS) radical scavenging activity, reducing power, and β-carotene bleaching assay. Results showed that anthocyanins exhibited excellent antioxidant activities in all assays and the EC₅₀ values of DPPH radicals scavenging, ABTS radicals scavenging, reducing power and β-carotene bleaching assay were 19.08, 10.69, 40.93, and 44.19 µg∙mL⁻¹, respectively. The Cd²⁺ chelation potency of anthocyanins was also investigated in vitro. Under given conditions, Cd²⁺ chelating ability of anthocyanins increased significantly with increase in contact time, anthocyanins concentration in dialysis tubing and Cd²⁺ concentration in solution. Based on these results, anthocyanins inducibly synthesized by Cd2 treatment was a powerful antioxidant, as well as Cd²⁺ chelator, might play a role in detoxification of Cd in A. imbricata.
EN
In the paper, the effects of differences in land use of a catchment and settlements on the variability of heavy metals concentrations (Zn, Pb, Cu, Cd, Ni) in the Narew River and some of its tributaries such as the Biebrza, upper Narew, Pisa, Omulew and Rozoga rivers were studied. The investigations continued from 1997 to 2002. The areas dewatered by the watercources differ considerably in terms of environmental features (forests, wetlands, bogs and water reservoirs), land use (arable lands, grasslands) and population (man-made areas). The total catchment of the Narew River at the cross-section in Zamski Kościelne was also taken into account. The urban areas make up from 0.5% of the Rozoga River to 2.5% of the upper Narew catchment. Water samples were collected quarterly (April, July, October and January) from four study sites located near the river mouth and were analyzed for Zn, Pb, Cu, Cd and Ni by the AAS method. The results showed that concentrations of Pb, Cu, Cd and Ni increased along the Narew River due to the accumulation of the elements in water. A significant influence on high heavy metal concentrations in water was produced by the town of Białystok, a source of large amounts of municipal pollutants discharged into the river. This mainly affected the content of Zn, as the highest pollution with this element was detected in the upper Narew River. The lowest Zn concentrations were observed in the Omulew River water, where forests dominated in the area, covering more than 48% of the catchment. The research showed that wetlands and lakes significantly decreased Pb, Cu, Cd and Ni concentrations in flowing water, but the presence of large inhabited areas with dense population and municipal pollution increased concentrations of heavy metals in river water.
PL
W pracy badano wpływ zróżnicowanego użytkowania obszaru zlewni oraz osadnictwa na zmienność stężenia metali ciężkich (Zn, Pb, Cu, Cd, Ni) w wodach Narwi i wybranych jej dopływów: Biebrzy, górnej Narwi, Pisy, Omulwi i Rozogi. Odprowadzają one wody z obszarów (zlewnie cząstkowe) znacząco zróżnicowanych pod względem warunków przyrodniczych (lasy, obszary podmokłe, bagna, zbiorniki wodne), sposobu użytkowania (grunty orne, użytki zielone) i zaludnienia terenu (tereny zantropogenizowane). Uwzględniono również zlewnię całkowitą Narwi po punkt pomiarowy w Zambskach Kościelnych. Na obszarze badań tereny zurbanizowane zajmują od 0,5% powierzchni w zlewni Rozogi do 2,5% w zlewni górnej Narwi. Badania obejmowały lata 1997-2002. Próbki wody pobierano z nurtu rzeki w punktach przyujściowych cztery razy w roku: wiosną (kwiecień), latem (lipiec), jesienią (październik) i zimą (styczeń). Oznaczenia Zn, Pb, Cu, Cd i Ni wykonano metodą absorpcyjnej spektrometrii atomowej. Wykazano, że wraz z biegiem rzeki Narwi, w wyniku kumulacji pierwiastków w wodzie, wzrastało stężenie Pb, Cu, Cd i Ni. Na wzrost stężenia metali ciężkich znaczący wpływ wywarła obecność w zlewni dużej jednostki osadniczej - miasta Białystok. Było to związane z dopływem większej ilości zanieczyszczeń bytowych powodujących wzrost stężenia Zn w górnej Narwi. Najniższe stężenie Zn stwierdzono w wodach odpływających Omulwią, w której zlewni dominowały lasy - ponad 48% powierzchni. Wykazano również, że występowanie terenów bagiennych, torfowych i zbiorników w zlewni wpływa na zmniejszenie stężenia Pb, Cu, Cd i Ni w wodach rzecznych, ale obecność dużych jednostek osadniczych i związany z tym dopływ zanieczyszczeń bytowych powoduje zwiększenie stężenia badanych metali ciężkich w wodzie rzecznej.
EN
Concentrations of dissolved and particulate cadmium were measured in the water of the Bah´ıa Blanca Estuary, one of the most highly industrialised regions in Argentina. Simultaneously, Cd contents were also determined in samples of phytoplankton. Thus, the estuary was characterised from the viewpoint of Cd occurrence. Additionally, a microscale environment was selected within the estuary in order to carry out an ecotoxicological study. Thus, environmental parameters (i.e. temperature, salinity, nutrients, chlorophyll a) were measured weekly at Puerto Cuatreros (in the inner estuary) during winter, when the phytoplankton bloom occurs; during the other seasons sampling was done twice a week. In addition, cells of the diatom Thalassiosira curviseriata – the dominant species in the phytoplankton bloom – were isolated and cultivated in the laboratory. The monoclonal cultures obtained were exposed to increasing concentrations of Cd under controlled environmental conditions and the growth rate inhibition was measured. As a preliminary conclusion it was found that Cd levels in the Bah´ıa Blanca Estuary are significantly lower than the EC50 value for T. curviseriata.
EN
The objective of our study was to investigate the activity of selected soil enzymes (dehydrogenases, phosphatases, urease, and protease), as well as cadmium and lead concentrations, in soils and vegetables from gardening allotments in areas exposed to industrial contamination and from areas of similar physiographic conditions but that were not exposed to the direct impact of industrial emissions. Investigations comprised eight gardening allotments situated in the area of Upper Silesia (Miasteczko Śląskie, Zabrze) and in cities in eastern Poland (Lublin, Stalowa Wola). Strong correlations were demonstrated between cadmium and lead concentrations in the soil and root systems of analyzed plants (red beet, carrot, parsley). The observed high inactivation of the examined enzymes in soils exposed to strong anthropogenic influence (the area of Upper Silesia) confirms that soil environment contamination with Cd and Pb reached levels that threaten living organisms.
EN
The effects of different concentrations (10,20,30,40 and 50 ppm) of cadmium chloride on seed germination, root length, shoot length, fresh weight, photosynthetic pigments, protein content and peroxidase activity in tomato (Lycopersicon esculentum L.). Higher concentration of Cadmium were reported specially at 40 and 50 μm reduced germination significantly. Leaf chlorosis, wilting and leaf abscission were observed in plants with cadmium. Protein content and sugar content were reduced in higher concentrations of cadmium. However, lower concentrations of CdCl2 resulted in higher peroxidase activity in roots and shoots of tomato.
EN
We examined cadmium (0.1 mmol/l) toxicity and effect of different calcium concentrations (0.1, 1.0, and 10.0 mmol/l) on the growth of organs (root, mesocotyl and coleoptile) of 4-day-old maize seedlings in hydroponic cultures. The influence of both metals on distribution of cadmium, calcium, potassium and sodium in the organs of maize seedlings was also studied. Calcium at a concentration of 1.0 mmol/l increased the growth of all organs in comparison with the growth in 0.1 and 10.0 mmol/l CaCl₂. Cadmium inhibited maize organ growth in the presence of 0.1 and 1.0 mmol/l CaCl₂, whereas cadmium toxicity was not observed at 10.0 mmol/l CaCl₂. It was found that the content of both metals in maize seedling organs rose with increasing concentrations of Ca or Cd in the hydroponic solutions. Cadmium administration together with 10.0 mmol/l CaCl₂ led to an increase in calcium concentrations in roots and coleoptiles. The highest calcium concentration (10.0 mmol/l) caused a decrease in the cadmium concentration in roots. The addition of cadmium with 0.1 or 1.0 mmol/l CaCl₂ diminished potassium accumulation in the roots. By contrast, potassium content in the roots was not altered by cadmium when calcium was applied at a concentration of 10.0 mmol/l CaCl₂. Sodium content in maize seedlings was not affected in the presence of both cadmium and calcium at a concentration of 0.1 or 1.0 mmol/l, whereas cadmium with 10.0 mmol/l CaCl₂ decreased sodium accumulation in the roots. Thus, we conclude that the positive effect of highest concentration of calcium on the growth in the presence of Cd relay on the maintenance of high concentration of potassium in the roots.
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