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1 Acta Biologica Cracoviensia. Series Botanica

1 2006

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Somatic embryogenesis from broccoli stigmas in tissue culture

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Zenkteler M. , Zenkteler E. , Dostatnia I.

Acta Biologica Cracoviensia. Series Botanica

2006

tom 48

nr 2

The factors regulating callus proliferation and bud regeneration from stigma tissues are not sufficiently understood. To study the regenerative capacity of pistil elements, pistil of broccoli was cultured under simple culture conditions. Stigmas with style from broccoli pistils undergo somatic embryogenesis on Murashige and Skoog basal medium. Callus initiation occurred on basal medium supplemented with 4 mg·l-1 BAP, 1.6 mg·l-1 2,4-D, 250 mg·l-1 casein hydrolysate and 30 g·l-1 sucrose. Proembryo induction was observed after two callus subcultures. Calluses with globular embryos were cultured on basal medium with 2 mg·l-1 BAP, 1 mg·l-1 IAA and 40 g·l-1 sucrose for development, maturation and germination of somatic embryos. A population of somatic embryos was maintained on medium containing 1 mg·l-1 BAP and 2 mg·l-1 NAA only. Adding NAA to MS medium containing BAP considerably enhanced root formation. After acclimatization, all plantlets developed well and produced phenotypically normal flowers.