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The influence of various concentrations of T-2 toxin on the growth ability and fermentation activity of brewing yeasts was examined. Four cultures of top and bottom fermenting yeast strains were maintained in YEPG medium containing T-2 toxin at the concentration of 2,5; 5; 10; 15; 20 i 50 μg·ml-1. The maximum specific growth rate (μmax) and biomass yield were determined. Concentrations of 5, 10 and 15 μg T-2·ml-1 were selected for the fermentation of malt wort. During top (20-22°C) and bottom fermentation (12-14°C), the following were examined: physiological condition of yeasts, fermentation dynamics and the degree of utilisation of amino acid nitrogen and the extract. It was observed that the presence of T-2 toxin in the medium resulted in lowered specific growth rate and biomass yield. T-2 toxin had an unfavourable influence on the physiological condition of yeasts and disrupted the mechanism of the intake of extract components, including amino acid nitrogen, which affected the dynamics of fermentation. Top fermenting yeasts, esp. S. cervisiae 46 strain, were more susceptible to T-2 toxin than bottom fermenting yeasts.
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