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EN
Convention on biological diversity of 5th June, 1992 including the regulations of the “International Treaty” on Plant “Genetic Resources” for Food and Agriculture prepared in Rome on 3rd November 2001 and the regulation of the Nagoya Protocol (of 29th October 2010) on access to genetic resources and the fair and equitable sharing of benefits arising from their utilization to the Convention on biological diversity will significantly affect the practice relating to the creative breeding of new plant varieties. Among the objectives of the above cited international legal acts “the fair and equitable sharing of benefits derived from the utilisation of genetic resources” is to be noted. Since utilisation of genetic resources is an essential component of the creative breeding of new plant varieties, the new, detailed legal regulations covering this area have to cause impacts on the breeding process and obtaining the exclusive plant variety right. The new legal regulations concerning protection of biodiversity will impact, in particular, on far–reaching formalisation of the breeding process through enforcing compilation and collection of the documentation connected with the genetic resources of origin used in the breeding process. Moreover, the way of participation of particular seed industry participants in profits connected with commercialisation of the exclusive plant variety right will also be subject to remodelling. Beside hitherto beneficiaries of such profits, i.e. breeders (holders of the exclusive plant variety right) and seed companies (licensees of the exclusive plant variety right) the group of entities salary–earning for the exclusive plant variety right will be also extended with entities providing genetic resources for the creative needs of plant breeding.
EN
In this study, LAMP markers linked to shelf-life in melon (Cucumis melo L.) were developed by converting a cleaved amplified polymorphic sequences (CAPS) marker (C2). The CAPS-PCR fragments from the long-shelf-life melon (0-3) and short-shelf-life melon (Nat-2) were cloned and sequenced to construct LAMP primers. A single nucleotide polymorphism (SNP) was identified between 0-3 and Nat-2. LAMP primers were designed to detect the SNP. In the LAMP reaction to detect long-shelf-life melon, the turbidity of the templates using 0-3, F1, homozygous long-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. In contrast, the turbidity of Nat-2 and homozygous short-shelf-life F2 lines did not increase even after 90 min. In the LAMP reaction to detect short-shelf-life melon, the turbidity of the templates using Nat-2, F1, homozygous short-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. But the turbidity of 0-3 and homozygous long-shelf-life F2 lines did not increase after 90 min. This attests to the high reliability and usefulness of LAMP for marker-assisted selection.
EN
The paper describes results of vegetative tests of activated carbon as a detoxiсant of herbicide remains in soil on eight varieties of summer rapeseed. The tests were performed with the purpose to align soil fertility on different breeding and test sites. The research is based on attempts to apply coal absorbent as a means of soil detoxication to neutralize herbicides remains in the process of oilseed brassica crops breeding. Several summer rapeseed varieties were used as research objects. Similar approaches have not encountered in literature references available.
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