Wyniki wyszukiwania - Biblioteka Nauki

1

An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue

100%

Rzepecka P. , Duda P. , Giebułtowicz J. , Sochacka M. , Friedman A. , Gałązka-Friedman J.

Nukleonika

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2017

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tom Vol. 62, No. 2

159--163

EN

Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investigations. Biological samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude possible artefacts from the background spectrum related to iron present in the counter and cryostat windows. The aim of this study was to compare two methods of determination of the amounts of iron in investigated sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the other, in which the obtained non-elaborated spectrum was fitted with two doublets – a doublet for the measured sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron (400, 800 and 1600 µg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed. Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99. Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the background spectrum had a significantly lower error of the final result.

2

Identification of brains tissue hyperelastic parameters

86%

Kuś W. , Poręba-Sebastjan M.

Journal of Applied Mathematics and Computational Mechanics

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2018

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tom Vol. 17, nr 3

53--60

EN

The goal of the paper is to correlate real brain deflection with its numerical model as the 3D model of a fragment of the brain and suction pipe. The model is analyzed with the Finite Element Method with use of Ansys software. The brain tissue can undergo large strains, which is why it is described by a hyperelastic material. The Mooney-Rivlin material model is used for numerical analyzes. The inverse problem is solved with use of optimization Non-Linear Programming by Quadratic Lagrangian (NLPQL).

3

Cystatin C and neuropeptid Y levels in brain tissues after experimental subarachnoid hemorrhage

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Acikgoz S. , Can M. , Guven B. , Edebali N. , Barut F. , Buyukuysal C. , Tekin I. O. , Acikgoz B.

Acta Biochimica Polonica

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2014

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tom 61

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nr 4

4

Gastrin-CCC-like immunoreactivities in the CNS of man

86%

Dorn A. , Rinne A.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 3-4

5

Aminobutyric Acid in Rat Brain Tissue Samples Using Benzoyl Chloride Derivatization and PDA Detection

86%

Akula K. K. , Chandrasekaran B. , Kaur M. , Kulkarni S. K.

Acta Chromatographica

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2015

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tom Vol. 27, no. 3

413--433

EN

A new, rapid, and specific reversed phase high-performance liquid chromatographic (RP-HPLC) method involving precolumn derivatization with benzoyl chloride was developed and validated for the estimation of γ-aminobutyric acid (GABA) in rat brain tissue preparations. The derivatization product of GABA was identified by melting point, infrared, and proton nuclear magnetic resonance (1H NMR) spectroscopy to be n-benzoyl GABA. Various parameters which influenced derivatization and elusion were optimized. The chromatographic system consisted of C-18 column with ultraviolet (UV)—photodiode array detection ranging from 210 to 400 nm. Elution with an isocratic mobile phase consisting of 0.025 M disodium hydrogen phosphate buffer—methanol (65:35, v/v; pH 6) at a flow rate of 1 mL min-1 yielded sharp and specific peak of n-benzoyl GABA within 7 min. The method was validated with respect to the linearity, accuracy, precision, sensitivity, selectivity, and stability, wherein the benzoyl derivative of GABA showed stability for 2 months. The lower limit of detection was 0.5 nmol L-1. This novel derivatization procedure for the estimation of GABA with benzoyl chloride was also applied for rat brain tissue preparations that gave highly specific peak and good component recovery. The results show that the method for the determination of GABA by benzoylation using RP-HPLC has good linearity, accuracy, precision, sensitivity, and specificity and is simple and economical to perform.

6

Histochemical investigation on dissociated cultures from neuroendocrine regions of fetal rat brain

86%

Pilgrim C. , Jirikowski G. , Reisert I.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 3-4

7

Localization of immunoglobulins G, A and M in glial cells of reactive and neoplastic origin

86%

Kaluza J. , Stachura J. , Junikiewicz E.

Folia Histochemica et Cytobiologica

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1985

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tom 23

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nr 4

8

Corpora amylacea from multiple sclerosis brain tissue consists of aggregated neuronal cells

86%

Selmaj K. , Pawlowska Z. , Walczak A. , Koziolkiewicz W. , Raine C. S. , Cierniewski C. S.

Acta Biochimica Polonica

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2008

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tom 55

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nr 1

EN

In this report, we describe proteomic analysis of corpora amylacea collected by postmortem laser microdissection from multiple sclerosis (MS) brain lesions. Using low level protein loads (about 30 µg), a combination of two-dimensional electrophoresis with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and database interrogations we identified 24 proteins of suspected neuronal origin. In addition to major cytoskeletal proteins like actin, tubulin, and vimentin, we identified a variety of proteins implicated specifically in cellular motility and plasticity (F-actin capping protein), regulation of apoptosis and senescence (tumor rejection antigen-1, heat shock proteins, valosin-containing protein, and ubiquitin-activating enzyme E1), and enzymatic pathways (glyceraldehyde-3-dehydrogenase, protein disulfide isomerase, protein disulfide isomerase related protein 5, lactate dehydrogenase). Samples taken from regions in the vicinity of corpora amylacea showed only traces of cellular proteins suggesting that these bodies may represent remnants of neuronal aggregates with highly polymerized cytoskeletal material. Our data provide evidence supporting the concept that biogenesis of corpora amylacea involves degeneration and aggregation of cells of neuronal origin.

9

Matrix-assisted laser desorption ionization time-of-flight mass spectrometric analysis of glycosphingolipids including gangliosides

86%

Taketomi T. , Hara A. , Uemura K. , Sugiyama E.

Acta Biochimica Polonica

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1998

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tom 45

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nr 4

EN

Long chain base compositions of gangliosides containing mainly stearic acid could be determined without any chemical modification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with delayed ion extraction (DE MALDI-TOF MS). The analytical results for the long chain base compositions of various samples of GM1 from the brain tissues of patients with different diseases at different ages confirmed that the proportion of d20:1 (icosasphingosine) and d20 (icosa-sphinganine) of the total sphingosine bases increased quickly until adolescent or adult age and then remained constant slightly exceeding 50%; this value was evidently higher than the proportion of d20:1 and d20 of GM1 in various adult mammalian brains. A long chain base composition of GM1 from the brain tissue of a patient with infantile type of GM1-gangliosidosis at 4y2m was abnormal and so was in two sibling patients with Spielmeyer-Vogt type of juvenile amaurotic idiocy at 19y and 21y in spite of that in the latter there was no accumulation of GM1 in the brain tissue. On the other hand, a patient with adult type of GM1 gangliosidosis at 66y showed a local accumulation of GM1 in the putamen and caudate nucleus, but its long chain base composition was found to be normal. It was of interest that the white matter of Eker rat with hereditary renal carcinoma contained a large amount of plasmalocerebroside as compared with the amount of cerebroside and sphingomyelin. The individual molecular species of plasmalocerebroside were identified by DE MALDI-TOF MS.

10

Activity of oxidative enzymes in neurolgia in vitro after hyperthermia

72%

Renkawek K. , Majkowska J.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 3-4

11

Membrane association of N-oleoyl-dopamine in rat brain

72%

Zajac D. , Matysiak Z. , Czarnocki Z. , Pokorski M.

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tom 57

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nr 4

403-408

EN

N-oleoyl-dopamine (OLDA) belongs to a novel class of bioactive amides of fatty acids. The compound, a lipid derivative of dopamine, holds promise as a potential prodrug or carrier of dopamine into the brain. In this context, a key issue concerning OLDA is the integrity of the compound once it enters the brain. We addressed this issue in the current study by assessing the propensity of OLDA for hydrolysis in rat brain tissue in vitro. The brains were dissected from surgically anesthetized rats after they had been sacrificed by perfusion with physiological saline through the heart. Membrane fractions of brain tissue were isolated and incubated with 1 mmol/l OLDA. Stability of the OLDA molecule was assessed from the spectrophotometric recordings of OLDA spectra in membrane fractions at hourly time points for up to 24 hours. The methodological assumption was that any major change in the shape of the OLDA spectrum would point to a structural, and thus also possibly functional, alteration of the molecule. We found that the OLDA spectrum remained unchanged in the assays for up to 17 h of incubation. We conclude that OLDA strongly resists hydrolysis in brain membrane fractions. The results suggest that dopamine-like biological effects of OLDA might have to do with the interaction of the integral OLDA compound, rather than a dissociated-off dopamine moiety, with the dopaminergic system.

12

Zastosowanie syntezy i analizy obwodów elektrycznych do wyznaczania oporów naczyniowych tkanki mózgowej

72%

Muc A. , Szarmach A. , Szurowska E. , Dzierżanowski J.

Pomiary Automatyka Robotyka

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2014

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tom R. 18, nr 3

107--111

PL

W pracy przedstawiono schemat obwodowy odwzorowujący przepływ krwi przez tkankę mózgową oraz metodę identyfikacji jego parametrów elektrycznych na podstawie rzeczywistych danych medycznych otrzymanych w wyniku zastosowania nieinwazyjnych procedur zabiegowych. Celem modelowania i identyfikacji jest usprawnienie diagnostyki patologii mózgowych o podłożu naczyniowym. Podczas identyfikacji uwzględniono średnie wartości regionalnego przeływu krwi (rCBF) i ciśnienia tętniczego (MAP), którym w schemacie obwodowym odpowiada stały prąd i napięcie. Dysponując jedynie stałym napięciem i prądem syntezowano obwód prądu stałego, w którym rezystancjom odpowiadają wypadkowe opory naczyniowe warstw skanowanej tkanki mózgowej. Praca została wykonana w ramach projektu „Interdyscyplinarna kadra akademicka na rzecz rozwoju gospodarki opartej na wiedzy” współfinansowanego ze środków Unii Europejskiej w ramach Europejskiego Funduszu Społecznego.

EN

In this study, the results of computed tomography perfusion (p-CT) was used to create a model of blood flow through the brain tissue as a constant current circuit. The equivalent electric circuit of the blood flow has been developed on the basis of similarities between electrical engineering and haemodynamics. Created model allows us to determine the additional hemodynamic brain blood flow in the form of resistance. The resistances in circuit are corresponding to vascular resistance for the individual layers and the entire scanned area of the brain. The mathematical model that results from the electric circuit, allows the analysis of the relationship between the layers of p-CT. The purpose of the modeling of brain tissue using an electrical circuit and then the identification of his parameters is a need to improve the diagnosis of cerebral vascular pathology. This work was financially supported by the European Community from the European Social Fund within the INTERKADRA project.

13

Effect of electromagnetic field exposure on mouse brain morphological and histopathological profiling

72%

Zymantiene J. , Juozaitiene V. , Zelvyte R. , Oberauskas V. , Spancerniene U. , Sederevicius A. , Aniuliene A.

Journal of Veterinary Research

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2020

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tom 64

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nr 2

14

The influence of heterocyclic compound - PAMAM dendrimer complexes on evoked electrical responses in slices of hypoxic brain tissue

72%

Potkin V. I. , Shcharbin D. , Denisov A. A. , Paschkevich S. G. , Bryszewska M. , Petkevich S. K. , Kletskov A. V. , Lapotko D. O. , Kazbanov V. V. , Gurinovich T. A. , Kulchitsky V. A.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 2

EN

We used complexes between a fourth generation polyamidoamine (PAMAM) dendrimer and one of two heterocyclic compounds — 1-(6-hydroxyhexyl)-3-(5-phenyl-isoxazole-3-yl)-urea or 5-phenyl-isoxazole-3-carboxylic acid — to reduce oxygen consumption in transverse slices of the hippocampus taken from 4-week old male rats. In vitro electrophysiological experiments revealed that the inhibitory effect of the hypoxic state on the evoked responses was enhanced in the presence of the complexes. The data were analyzed in terms of the potential antitumor effects of these complexes.

15

Spatio-temporal pattern of proliferation of immunoglobulin G - containing astrocytes in the injured mouse cerebral hemisphere

72%

Janeczko K.

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tom 33

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nr 3

16

Mobilization of human hematopoietic stem-progenitor-enriched CD34plus cells into peripheral blood during stress related to ischemic stroke

72%

Machalinski B. , Paczkowska E. , Koziarska D. , Ratajczak M. Z.

Folia Histochemica et Cytobiologica

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2006

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tom 44

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nr 2

17

Comparison between Fe, Zn, Cu, and Mn concentrations in brain hemispheres and stem of two wild geese species of the genus anser

72%

Kalisinska E.

Biological Bulletin of Poznań. Supplement

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1996

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tom 33

18

Bulbospinal catecholamine neurones and sympathetic pattern generation

72%

Coote J. H. , Lewis D. I.

Journal of Physiology and Pharmacology

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1995

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tom 46

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nr 3

19

2-chloro-2'-deoxyadenosine [2-CdA] combined with cyclosporine A successfully prevents rejection of fetal brain stem allograft in rabbits

72%

Grieb P. , Ryba M. , Janczewski W. , Sawicki J. , Jagielski J. , Andrychowski J.

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tom 42

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nr 1

20

Obidoxime content in the brain tissue of rats intoxicated with organophosphate insecticide chlorfenvinphos after administration of Toxogonin

72%

Raszewski G. , Burda S.

Acta Toxicologica

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2006

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tom 14

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nr 1-2