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1 Bulletin of the Veterinary Institute in Puławy

1 2001

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Effect of infection with bovine immunodeficiency virus and-or bovine leukaemia virus on lymphocyte blastogenesis to mitogens in sheep

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Stec J. , Kuzmak J.

Bulletin of the Veterinary Institute in Puławy

2001

tom 45

nr 1

Lymphocyte blastogenesis was determined by ³H-thymidine incorporation assay after stimulation of the cell cultures with mitogens: Concanavalin A, phytohaemagglutitin, and pokeweed mitogen. BIV and/or BLV infection were associated with significant changes in lymphocyte blastogenesis to mitogens. The highest responses to all three mitogens were shown by 3 to 4.5 months after infection when compared to control animals, and thereafter were depressed at the end of the experiment Stimulated lymphocytes revealed in 20 - 50 fold increase in blastogenesis over background based on the unstimulated cells. These results demonstrated that a function impairement of lymphocytes can be observed in the course of BLV and/or BIV infection.