Wyniki wyszukiwania - Biblioteka Nauki

1

Obtaining healthy offspring from PRRSV-positive pig breeding herds

100%

Lustyková A. , Frydrychová S. , Seifert J. , Daněk P. , Rozkot M. , Lustyková A. , Frydrychová S. , Seifert J. , Daněk P. , Rozkot M.

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nr 4

2

Evaluation of two prototype directional freezing methods and a 2ml flattened straw for cryopreservation of boar semen

75%

Puglisi R. , Bornaghi V. , Severgnini A. , Vanni R. , Montedoro M. , Galli A.

Animal Science Papers and Reports

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2017

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tom 35

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nr 4

EN

Based on previous assessments on stallions, 40 ejaculates of 20 Duroc boars were split and evenly frozen with a conventional vapour freezing method and two directional, drum and directional, prototype methods using commercial extenders and relative standard procedures. The directional prototype was provided with a double internal setup that allowed the positioning of experimental 2ml flat straws with 1 billion sperm (Flat) in a fixed support, or both classical 0.5 ml paillettes with 250 million spermatozoa and flats in a rotating drum designed so as to ensure a more uniform heat exchange. Preliminary tests for individuation of the most appropriate thawing rate showed beneficial effects (P≤0.05) of thawing the sperm at 50°C for 13 s when compared to 42°C for 20 s, in terms of total motility (42.8±8.4% and 35.6±6.8%, respectively). With regard to freezing/packaging methods, major improvements (P≤0.05) were shown for the drum method with paillettes for total motility (38.6±14.2%) assessed immediately after thawing, when compared with the conventional (29.4±13.3%) and the directional methods with flats (30.2±12.8%), and for total motility (P≤0.01) assessed following incubation for 120 min at 37°C after thawing (24.8±11.6%) with respect to the conventional method (15.6±10.9%). Despite the statistical non-significance of results, both the prototype freezing approaches using the experimental flat straw showed some improvements in functional parameters assessed by cytofluorometry when compared to the conventional method.

3

Analysis of relations between polymorphism in ryanodine receptor gene [RYR1] and certain characters of boar semen in Polish Landrace pigs

75%

Kmiec M. , Dybus A. , Wierzbicki H. , Terman A. , Ziemak J.

Acta Scientiarum Polonorum. Zootechnica

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2003

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tom 02

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nr 1

4

Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe

63%

Fraser L. , Zasiadczyk L. , Strzezek J.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 2

292-298

5

Effect of boar breed and chosen crossing variants on the development of basic traits of boar semen

63%

Szostak B. , Sarzynska J.

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2008

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tom 26

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nr 4

10-16

6

Ferrous ion induced photon emission as a method to quantify oxidative stress in stored boar spermatozoa

63%

Gogol P. , Pieszka M.

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tom 56

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nr 3-4

173-177

7

Morphological changes in wild boar (Sus scrofa L.) semen in annual cycle

63%

Kozdrowski R. , Dubiel A.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2004

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tom 07

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nr 2

8

Effects of decondensing agents on chromatin stability of boar spermatozoa - radioisotope study

51%

Strzezek J. , Kordan W.

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2003

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tom 21

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nr 3

EN

Chromatin stability is an important determinant of semen quality, essential for spermatozoa maturation in epididymes and early embryogenesis. A radioisotope method based on the quantitative measurements of tritium-labelled actinomycin D (3H-AMD) incorporation into the spermatozoa nuclei was used to assess chromatin stabilization of boar spermatozoa incubated with physiological (reduced glutathione – GSH, heparin – H and bosine serum albumin – BSA) or non-physiological (dithiothreitol – DTT, disodium ethylenediaminetetra acetate – EDTA, 2-mercaptoethanol – ME and sodium dodecyl sulphate – SDS) decondensing agents.The effect of the composition of seminal plasma and the role of zinc ions in chromatin stability of spermatozoa were also studied. Pre-treatment of spermatozoa with GSH, H, DTT, ME or SDS resulted in an increase in the incorporation of 3H-AMD into the spermatozoa nuclei. In contrast, when sperm samples were treated with BSA or EDTA there was a reduction in the incorporation of 3H-AMD, what was attributed to hyperstabilization of chromatin. A presumed hyperstabilization was also observed when SDS+EDTA+H were used. On the other hand, an exceptionally strong action of decondensation of chromatin was induced by H+BSA. Increased incorporation of 3H-AMD into the spermatozoa nuclei was concomitant with low zinc and protein content in the seminal plasma of boars following depletion test (DT), suggesting disturbances in chromatin stability. The presented radioisotope method based on the application of 3H-AMD is a simple and reliable assay that can be used to monitor the chromatin status of boar spermatozoa.

PL

Stabilność chromatyny plemników jest istotnym wyznacznikiem jakości nasienia, bowiem determinuje przebieg procesu dojrzewania plemników w najądrzach oraz wczesny rozwój zarodkowy.W badaniach zastosowano radioizotopową metodę pomiaru wiązania 3H-aktynomycyny D (3H-AMD) przez jądra plemników inkubowanych z czynnikami dekondensującymi chromatynę: fizjologicznymi (zredukowany glutation – GSH, heparyna – H, albumina surowicy bydlęcej – BSA) i niefizjologicznymi (ditiotreitol –DTT, wersenian dwusodowy – EDTA, merkaptoetanol – ME, laurylosiarczan sodowy – SDS). Badano wpływ składu plazmy nasienia na stabilność chromatyny plemników, ze szczególnym uwzględnieniem funkcji jonów Zn2+. W przypadku zastosowania GSH, H, DTT, ME i SDS stwierdzono wzrost wiązania 3H-AMD przez chromatynę plemników. Po inkubacji plemników z BSA lub EDTA obserwowano spadek wiązania. Pod wpływem grupy czynników dekondensujących w mieszaninie inkubacyjnej: SDS-EDTA-H,w nieobecności DTT (specyficznie rozszczepiającego mostki -S-S-) obserwowano spadek wiązania 3HAMD, co zinterpretowano jako hyperstabilizację chromatyny. Silne działanie dekondensujące chromatynę przez kompleks czynników fizjologicznych BSA-heparyna, uznano za skutek ich działania jako układu ligandów wiążących Zn2+, co sprzyjało destabilizacji wiązań S-Zn-S.Dziesięciodniowe próby opróżniania knurów, czemu towarzyszyło stopniowe obniżanie produkcji plemników i zdolności sekrecyjnej dodatkowych gruczołów płciowych, były sprzężone ze spadkiem wiązania 3H-AMD. Wyraźne obniżenie zawartości białka całkowitego i koncentracji jonów Zn2+ w plazmie niasienia obserwowane podczas prób opróżniania podkreśla udział tych komponentów w stabilizacji chromatyny plemników knura.Zastosowana metoda radioizotopowa z użyciem 3H-AMD może być wykorzystana w biochemicznej ocenie stanu chromatyny plemników.

9

Fertilizing capacity of boar semen frozen in an extender supplemented with ostrich egg yolk lipoprotein fractions - A pilot study

51%

Fraser L. , Strzezek R. , Strzezek J.

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tom 10

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nr 3

EN

A limited field trial was performed to evaluate the fertilizing capacity of boar spermatozoa frozen in an extender supplemented with lipoprotein fractions isolated from ostrich egg yolk (LPFo). Boar semen, diluted in an extender containing lactose with lyophilized lipoprotein fractions, glycerol and Orvus Es Paste (lactose-LPFo-G), was frozen using a controlled programmable freezer. Sperm characteristics, such as motility, plasma membrane and acrosome integrity, and mitochondrial function were monitored. Post-cervical artificial inseminations (post-CAIs) in multiparous sows (Polish Large White) were performed using the Soft & Quick® catheter/cannula set. Sows were inseminated 2 to 3 times within one oestrus. Possible returns of sows to oestrus were determined from 21 to 30 days after post-CAIs. In this field trial, sows inseminated with 2 x 109 motile frozen-thawed spermatozoa resulted in pregnancy and farrowing rates of 75%, respectively. The average piglets born live was 10.5 ± 0.4 (mean ± SEM). The data of this study showed that post-CAI of boar semen frozen in LPFo-containing extender has the potential to provide acceptable fertility results. Further investigations are needed to elucidate the cause of variations in pregnancy/farrowing rate associated with frozen-thawed boar semen.

10

Concentrations of carnosine, anserine, L-histidine and 3-methyl histidine in boar spermatozoa and sheep milk by a modified HPLC method

51%

Ducci M. , Pacchini S. , Niccolini A. , Gazzano A. , Cerri D. , Gadea J. , Bobowiec R. , Sighieri C. , Martelli F.

Polish Journal of Veterinary Sciences

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2006

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tom 09

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nr 3

EN

The present study deals with the application ofhigh-perform ancc-liquid-chromatographyl(HPLC) method for a quantitative detection of carnosine, anserine, L-histidine and 3-methyl-L-histidine in biological material with o-phthaldialdehyde (OPA) post column derivatisation at the constant temperature of 50°C. For this purpose, some mobile-phases were prepared with scalar acetonitrile concentrations. A complete separation of all molecules, particularly for carnosine and 3-methyl-L-his- tidine, was obtained with a solution of acetonitrile and 6mM hydrochloric acid with 0.48 M sodium chloride (5%:95% v/v). Post column derivatisation reaction at temperature of 50°C permitted to obtain an increase in sensibility of all molecules. This method has been utilised for detection of histidine dipeptides in boar spermatozoa and in sheep milk. Concentrations (mean ± S.E. nmol/109 spermatozoa) of carnosine (0.96 ± 0.14) and anserine (0.83 ± 0.18) in boar spermatozoa were significantly lower than those of L-histidine (52.85 ± 4.86) and 3-methyl-L-histidine (83.07 ± 7.1). Positive correlation was found between carnosine and anserine contents (r=0.740; p<0.01) and between L-histidine and 3-methyl-L-histidine (r=0.657; p<0.01). All histidine dipeptides studied were also present in 40 samples of sheep milk. In a case of samples without unit-forming colonies (UFC) of Staphylococcus coagulase-positive, carnosine concentrations (9.17 ±0.89 nmol/ml) were higher than anserine (0.51 ±0.02 nmol/ml) and both were significantly lower in respect to L-histidine (49.51 ± 6.48 nmol/ml) and 3-metyl-L-histidine (81.21 ±6.82 nmol/ml). A negative correlation was observed between carnosine milk levels (r=-0.773; p<0.01) and UFC/ml of Staphylococcus coagulase-positive. In conclusion this very simple and fast method can be used to detect histidine dipeptides in biological compartments where their concentrations are very low.

11

Wplyw roznych metod konserwacji nasienia knura w niskich temperaturach na wybrane wlasciwosci plemnikow

32%

Bielas W.

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2002

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tom 01

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nr 2

13-22