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Activity of T and B lymphocytes and blood phagocytes in pigs immunomodulated with bioimmuno and/or immunised with respisure one vaccine against mycoplasmal pneumonia of swine

100%

Procajlo Z. , Szweda W. , Siwicki A. , Platt-Samoraj A. , Mikulska-Skupien E.

nr 4

The purpose of the studies was to determine the proliferation activity of T and B lymphocytes as well as the metabolic and potential killing activity of blood phagocytes in pigs after the immunomodulation with the Bioimmuno preparation and/or immunisation with the 'Respisure One' vaccine against mycoplasmal pneumonia of swine. The studies were performed on piglets at the age of 4 weeks, divided into four groups of seven animals each. The biopreparations were administered according to the following pattern: Bioimmuno (1 kg/50 kg of feedstuff) for 48 h before vaccination with Respisure One (2 ml/animal i.m.) on the 28th d of life (group I), Bioimmuno (1 kg/50 kg of feedstuff) on days 26 and 27 of life (48 h before vaccination with Respisure One of groups I and III) (group II), Respisure One on the 28th d of life (group III) and PBS (2 ml/animal i.m.) simultaneously with vaccination of groups I and III (group C - control). The proliferation activity of T and B lymphocytes (MTT test) and the metabolic and potential killing activity of blood phagocytes (RBA and PKA tests) were determined in whole blood. Statistically significantly higher (P<0.05) proliferation activity of Con A-stimulated T lymphocytes as well as LPS-stimulated B lymphocytes were found in the stimulated and immunised group I and the immunised group III in comparison with the stimulated group II and group C. Higher metabolic and potential killing activities of the phagocytes were also detected in all experimental groups compared with the control one. The studies have demonstrated that the Bioimmuno immunomodulator and/or the Respisure One vaccine stimulate the proliferative response of T and B lymphocytes, as well as they increase the metabolic and potential killing activity of blood phagocytes, contributing to the improvement in the immune system functioning and to the protection of the swine organism against the infections of the respiratory system.

The ingestion of Aeromonas salmonicida subsp. salmonicida by fish blood phagocytes in vitro under influence of herbicides

100%

Kolman H. , Terech-Majewska E. , Kolman R. , Szarek J. , Swiatecki A.

nr 1