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EN
Lymphocytes of haematologically and serologically EBB-positive cows were isolated and incubated with CD4, CD8, Cdllc and alpha bovine IgM markers, produced by Int. ILRAD Peter Maesseus. The cells were analysed immediately following fluorescein marking, in flow cytometer (FACS Calibur - Becton Dickinson). The number of proliferating B lymphocytes with increased IgM marker expression was high, testifying to early infection of the cattle with bovine leukemia virus (BLV). The expression of CD4 and CD8 markers in Th and Tc lymphocytes in leukemic cows was more pronounced than in healthy animals. The changes were accompanied by a decreased production of natural killer (NK) cells.
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nr 1
129-134
EN
The effects of aflatoxin B₁ (AFB₁), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), and zearalenone (ZEA) on in vitro response of pig peripheral blood mononuclear cells to mitogen concanavalin A was assayed after three days of incubation using ³H- thymidine uptake. Dose response curves for each mycotoxin were generated and the concentrations producing 50% inhibition of cell proliferation (IC₅₀) were estimated. AFB₁, OTA, and PAT were the most potent toxins with the IC₅₀ of 0.06, 0.17, and 0.19 µmol/L, respectively (0.2, 0.7, and 0.3 µg/mL, respectively). Based on the molar concentration, the inhibition potencies relative to that of AFB₁ were determined. OTA had 35% and PAT 31% potency to that of AFB₁, but CIT and ZEA had only 1.6 and 1.9 of AFB₁ inhibition potencies.
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