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Content available remote Human cytomegalovirus DNA level in patients with idiopathic pulmonary fibrosis
72%
EN
The objectives of the study were to estimate human cytomegalovirus (HCMV) DNA copy number in broncho-alveolar lavage cells, blood leukocytes, and serum of patients with idiopathic pulmonary fibrosis (IPF). The study groups consisted of 16 patients, newly diagnosed with IPF and never treated, (mean age 40.9 ±11.0 yr; F/M-7/9) and in 16 adult healthy volunteers (mean age 36.8 ±6.4 yr; F/M-4/12) used as controls. The HCMV DNA copy number was calculated by a Q-PCR method using TaqMan ABI PRISMTM7700. We found that the prevalence of the HCMV DNA positive subjects in the patient group (75%) did not differ significantly from that in the control group (69%). We also found that in both patient and control groups the mean HCMV DNA copy number in BAL cells was significantly higher than that in blood leukocytes (log10=2.7 vs. 1.2 for patients and 2.8 vs. 0.9 for controls, respectively). However, a higher HCMV DNA copy number in blood serum was observed in IPF patients than in controls (log10=3.2 vs. 2.0, respectively). We conclude that the lungs play an important role in the human pathobiology of cytomegalovirus sustenance.
EN
The aim of the study was the evaluation of DNA vaccine against CSF and the estimation of its safety, protection value and immunogenicity. Piglets were vaccinated with the gene encoding the gp55 glycoprotein of CSFV with signal sequence and without the transmembrane region. For comparison the whole gene of gp55 glycoprotein and the DNA of plasmid vector only were used. For the challenge virulent Washington strain of CSFV was used. Clinical observations, body temperature and the immune response (haematological and FACS analyses, presence of virus-specific antibodies) were monitored. The presence of the virus antigen in blood, nasal swabs and organs of dead animals was checked by virus isolation, antigen ELISA and PCR methods. Only pigs vaccinated with the first construct were protected from the challenge, however, two-day fever > 40°C was registered. In other groups typical clinical and pathological symptoms accompanied by significant leukopenia were evidenced.
EN
In Black-and-White cattle, polymorphism of acid phosphatase (AcP) of blood leukocytes is determined by a pair of autosomal alleles. The aim of the study was to determine the relationship between AcP polymorphism and the metabolic efficiency of phagocytes in the first months after calving of cows naturally infected with the bovine leukaemia virus. The studied population consisted of 91 Black-and-White cows aged 3-6 years, from one herd. Enzootic bovine leukaemia (EBL) was diagnosed with the immuno-enzymatic ELISA method and a PCR molecular test. Additionally, agarose gel electrophoresis and the cytochemical method were used to determine the AcP polymorphism and activity in leukocytes. The metabolic activity of phagocytes was determined by the nitroblue tetrazolium (NBT) reduction test. Significant differences in metabolic efficiency of granulocytes were observed between cows representing different AcP phenotypes. No significant differences in levels of the analysed indices were observed between the EBL-positive and EBL-negative cows and between the three subsequent months after calving.
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