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PL
Ekotoksykologia jest nauką względnie młodą, która stanowi połączenie ekologii i toksykologii, pozwalające na badanie wpływu substancji toksycznych na ekosystemy. Bioindykacja jest metodą, w której wykorzystuje się jako wskaźnik organizmy żywe. Ich reakcja może być podstawą do oceny ogólnej aktywności biologicznej badanego układu, a co za tym idzie, do oceny toksyczności badanej próbki. Za pomocą bioindykacji można wyznaczyć ogólną toksyczność badanej próbki oraz określić sumaryczny efekt oddziaływania zanieczyszczeń zawartych w ściekach na środowisko wodne. Istnieje wiele testów, w których jest wykorzystywana metoda bioindykacji. W Głównym Instytucie Górnictwa badania prowadzono z zastosowaniem LUMIStox. System ten pozwala na uzyskanie powtarzalnych i miarodajnych wyników pomiarów.
EN
Ecotoxicology is relatively young science, which forms link between ecology and toxicology enabling investigation of toxic substances’ impact on ecosystems. Bioindication is a method in which living organisms are used as indicator. Their reaction can be a basis for assessment of general biological activity of studied system, and, as a consequence, for assessment of studied sample toxicity. With the help of bioindication, the general toxicity of studied sample may be determined, as well as the total effect of contaminations contained in sewages impact on water environment may be defined. Many tests exist, in which the bioindication method is used. In Central Mining Institute, the tests with the use of LUMIStox were conducted. This system enables to obtain repeatable and reliable results of measurements.
EN
The article deals with the development of new eco-innovative technologies, evaluation of their effectiveness and raising the widest possible awareness of those categories to the people who could effectively use the proposed technologies. It was found that the personal position of economic entities is the core of the structural organization of psychological readiness for ecologically oriented innovation and is manifested in the forms of free ecologically oriented will. The paper discusses the main methods of removal of nutrients from wastewater, as well as presents promising technologies and techniques. It contains the recommendations for optimal conditions for the precipitation of ammonia nitrogen from an ion exchange regenerator for further use as an ammonium fertilizer. The maximum efficiency of ammonium nitrogen removal was established. According to the results of the best options, the deposition of real ion exchange concentrates was carried out. The humidity level was set during the drying of mineral fertilizers. The moisture content determined the content of sediment, which is close to the number of water molecules to the formula of the fertilizer MgNH4PO4 · 5H2O. Complex phosphate fertilizer struvite was characterized. A comparative agronomic evaluation of synthetic struvite was performed. The influence of struvite on the processes of germination of cultivated plants was carried out. The results show that the method of obtaining struvite does not significantly affect the processes of wheat germination, and the determining factor in the effectiveness of fertilizers is the method of its application and dosage.
PL
Celem prezentowanej pracy jest zidentyfikowanie obszarów rolniczych zagrożonych zanikaniem różnorodności gatunkowej na przykładzie zgrupowania dżdżownic traktowanego jako zastępczy indykator różnorodności gatunkowej organizmów glebowych. Przeanalizowano najważniejsze cechy charakteryzujące bieżące tendencje w zakresie przestrzennego zróżnicowania rolnictwa w Polsce, a także przeprowadzono szczegółowe badania z wykorzystaniem metody bioindykacyjnej. Wyniki tych dwóch analiz stanowiły podstawę do wskazania trzech typów obszarów o dużym prawdopodobieństwie nasilania się procesów upraszczania struktury krajobrazu, a więc i ryzyka utraty różnorodności biologicznej gleby.
EN
The aim of this study is to identify the agricultural areas threatened by biodiversity decline on the example of earthworm assemblages treated as a surrogate indicator of soil organisms’ diversity. Authors carried out an analysis of characteristics of current trends in spatial structure of agriculture in Poland. Moreover, detailed study based on the bioindication method was conducted. The results of these two studies were the basis for identifying three types of areas with a high probability of intensification of the process of simplifying the landscape structure, and thus the risk of soil biodiversity loss.
EN
The aim of this study was to compare colonization of pine chips without supplements and pine chips supplemented with forest litter, peat and lignite by mites (Acari), and particularly oribatid mites (Oribatida) in a two-year cycle. The study was conducted in the years 2013-2014, on microplots established in a belt of trees in a nursery in Białe Błota within Bydgoszcz Forest District. The experiment was established on four microplots (1 x 1 m). It included the following variants: C - pine chips alone, Lf – pine chips inoculated with fresh forest litter, Lf+Pe – pine chips enriched with deacidified high peat (20%) (pH 5.5-6.5) and inoculated with the litter, Lf+Li - pine chips enriched with granulated lignite (20%) inoculated with the litter. In the first year of the study, mite abundance in the chips inoculated with forest litter was significantly higher than that on microplot C. In the second year of the experiment, the abundance of these microarthropods decreased probably due to unfavorable weather conditions. The most common group of mites were usually oribatid mites that accounted for 19.7 to 80.4% of all mites. An analysis of seasonal dynamics of Oribatida abundance over the entire study cycle revealed a clear increase in their numbers in non-supplemented pine chips only on the last sampling date. Oribatida abundance in Lf variant was similar at the beginning and end of the study. Contrary to that, their number decreased in the chips supplemented with peat, and particularly those enriched with lignite. In total, 36 species of oribatid mites were identified on all microplots. The greatest boost in species diversity after the introduction of forest litter was observed in Lf chips and the smallest in Lf + Li variant. Oribatula tibialis was the most common oribatid mite in the investigated substrates. Low numbers of Oppiella nova and Tectocepheus velatus, the species having trophic associations with fungi, may indicate poor colonization of the chips, particularly those enriched with lignite, by saprotrophic organisms.
EN
The study was conducted on the upper reach of the Czarna Maleniecka River from Furmanów to the ford at Piekło. Nine sampling sites were selected along this 20-km section. Detailed studies of flora and the physicochemical water parameters were performed in the 2004- 2006 period. Data on macrophytes were collected with the Mean Trophic Rank (MTR) method. The results of physicochemical measurements were analyzed in reference to the water quality classification of the Polish Ministry of the Environment. Based on the results of these studies, the authors recommend adding two species, Utricularia intermedia and Glyceria fluitans, to the list of bioindicators.
EN
There is a cascade of hydroelectric power plants built on the Váh River. From a water-management point of view, the natural channel is used to drain extreme discharges. During most of the year, discharges are regulated by water-management structures. These discharges are not used for energy-related purposes; therefore, it is important to determine the optimal discharge that will not negatively affect the ecosystem of the stream. The minimum balance discharge (hydro-ecological discharge) was determined based on the instream flow incremental methodology (IFIM) using the riverine habitat simulation system (RHABSIM). Input data were obtained from direct measurements on three reference reaches in the area between the cities Piešťany and Nové Mesto nad Váhom. Hydraulic flow characteristics were derived from three measurements at different water levels. Habitat quality was represented by ichthyofauna. Data to determine the habitat suit-ability curves of fish were obtained using a diving technique to collect video footage. The modelling resulted in the quantification of the effect of discharge on ichthyofauna as a bio-indicator of habitat quality, which implied the need of 20 m3∙s–1 for a minimum balance discharge in summer.
EN
One of the widespread sources of river pollution is the wastewater coming from both wastewater treatment plants and the stormwater system. Wastewater can vary significantly in composition and concentration of substances introduced into water bodies. Municipal effluents may contain significant amounts of organic matter and ammonia. Storm drains are diverse in composition and depend on the nature of the surface from which the water collects, but carry more suspended solids and less nutrients. The research was aimed at assessing the effect of surface runoff collected by the stormwater system from the territory of the city of Lublin on the Bystrica River using popular environmental indices, calculated on the basis of periphytonic algae species abundances: species number, Shannon’s H, rarefied species number, Pielou’s evenness, trophic diatom index (TDI). It was observed that the correspondence between a species diversity and the quality of the environment is not always straightforward. Therefore, the periphytonic algae diversity increases under the influence of runoff, as evidenced by the Shannon index. Nevertheless, pronounced changes are noted in the structure of the algal community, as shown by the Pielou index and NMDS. However, these changes in the structure are invisible if the trophic diatom index (TDI) is relied upon.
EN
The study was conducted in the Pomological Orchard of the Research Institute of Horticulture in Skierniewice on a plantation of blackcurrant cultivar ‘Tiben’. The following variants of the experiment were applied: control – NPK fertilization only, mulching with a peat substrate, shredded pine bark, sawdust of coniferous trees, bovine manure, plant compost, straw of cereals, and application of mycorrhizal inoculant MYKOFLOR. Mulching was performed each year in the spring in an amount of 25 dm3 per plot, and the inoculum was applied in an amount of 10 ml per shrub. Samples for acarological examinations were collected at 4 times, in the spring and autumn of successive seasons in 2012-2013. An increase in the overall density of mites, in comparison with the control surface, was observed after mulching the soil with sawdust of coniferous trees and plant compost. On all the plots, the communities of mites were dominated by mites of the order oribatid mites. For these mites, a statistically significant increase density was recorded after mulching the soil with sawdust of coniferous trees, bovine manure and plant compost. Mulching did not increase significantly the species diversity of oribatid mites. The soil of the blackcurrant plantation was found to be inhabited by relatively large numbers of two common oribatid mites species: Punctoribates punctum and Tectocepheus velatus. An increase in the population density of Punctoribates punctum was observed after mulching with peat, sawdust and compost.
EN
One of the important ways to prevent permanent environmental pollution is to constantly monitor its quality, which can be performed in several ways. The present bioindication study analyzed the level of diversity and abundance of biofilm microorganism communities, which illustrate the state of the studied aquatic environment, enabling to determine its quality. The impact of stormwater discharge on the receiver at particular points was evaluated on the basis of the reaction of selected microorganisms or their groups to the substances appearing in the watercourse. The study of indicator organisms gives information about the waters of a given body of water without expensive hydrochemical tests and without causing a burden on the environment during the production as well as disposal of reagents that are consumed in many classical physicochemical analyses. On the basis of selected algal species, the Shannon index and McArthur index were calculated, and the effect of storm sewer discharge on the communities of indicator organisms was determined. The best visible impact of storm sewer discharge was seen on the basis of the entire study cycle in relation to the median of the McArthur index.
11
Content available Effect of Stormwater System on the Receiver
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EN
The surface water quality assessment could be based on a combined physical and chemical analysis, but it could also be determined with bioindication methods. Classical physicochemical analysis is in most cases more expensive and time-consuming than the bioindication methods. This type of analysis also requires expensive equipment and shows the situation in the water only at the moment of sampling. Although the bioindication methods are often complicated, they allow a relatively inexpensive estimation of the water quality. Moreover, during their implementation, the substances harmful to the environment are not generated, and the obtained results usually reflect the total interaction of all factors and substances to the analyzed living organisms. Indicator organisms or their communities applied to the research, with identified ranges of tolerance to selected factors, could help to determine the physical and chemical parameters of water. This paper presents a bioindication study with an effect of stormwater system on the receiver – the Bystrzyca river, in Lublin, Poland. The level of saprophyty of the river sector was calculated based on the selected species of algae (diatoms and green algae) and the influence of the stormwater discharge on the communities of these organisms was determined.
EN
The article deals with the main methods of nutrient removal from wastewater, as well as presents promising technologies and techniques. Struvite was characterized by a complex phosphorus fertilizer. The influence of struvite on the processes of germination of cultivated plants was investigated. The possibility of creating a growth substrate as an inexpensive and effective alternative to the disposal of the man-made wastes that contain an organic component was identified. The composition of the growth substrate was selected to ensure its functional properties. The growth substrate was tested by bioindication. Subsequently, the modification of the substrate by adding natural sorbents.
EN
KopyrinaA total of 437 species (453 with infraspecific taxa) of algae and Cyanobacteria were identified in 2034 samples collected during the first research on nine thermokarst lakes in Yakutia carried out in 1986–1994 and 2001–2015. The richest algal flora was determined in Lake Aalah, represented by 241 taxa. Bacillariophyta species dominated in four lakes, whereas Chlorophyta and Cyanobacteria dominated in the others. The algal bioindication demonstrated preferences for benthic and plankticbenthic life, temperate temperature, low alkalinity, waters with moderate oxygenation, low to medium enrichment with chloride, low organic pollution, and mesotrophic state. Comparative floristic, CANOCO and JASP statistical methods divided the species and environmental variables into groups of lakes related to landscape terraces. Local landscape positions and high summer water temperatures may play a major role in the formation of algal flora. The index of algae taxa per area stabilized at about 70 species per km2. The highest polymorphism of algae floras, defined by the Subspecies/Species Index, was determined for the lakes of the Tungulunskaya terrace: Lake Ynakh, Lake Nal Tungulu and Lake Tungulu (1.041–1.058), and the average total flora was 1.036. Our results can help in monitoring and protecting the ecosystems of thermokarst lakes in Yakutia in the harsh climate environment of Northeastern Eurasia.
EN
The study was conducted within the Bydgoszcz limits and in the vicinity of city Bydgoszcz at seven different habitats of Hierochloë repens presence: city district Fordon - (1), (2), (3) and (4); city district Kapuściska - (5), and Białe Błota near Bydgoszcz - (6) and (7). Samples for acarological analyses were collected from the plots covered with Hierochloë repens in spring (27 May) and autumn (28 October) 2014. The density of oribatid mites on the sites with Hierochloë repens was highly variable. High density of these microarthropods was characteristic of plots (2) with Convolvulo-Brometum inermis, (5) with Arrhenatheretum elatioris and (7) covered with grassy herbaceous plants growing on fertile soils. Particularly low density of oribatid mites was observed for plot (3) with Koelerio-Corynephoretea and Festuco-Brometea communities and the lowest content of carbon and nitrogen in the soil. Depending on the site and sample collection time, four to 22 species of oribatid mites were identified. Statistical analysis (Mann-Whitney U test) of mean number of species per sample s identified two groups of plots for both sample collection terms characterized by either low (1.00-3.10) or high values (3.30-7.40) of this parameter. The first group included plots (1), (3) and (6), and the second (2), (4), (5) and (7). The following species of oribatid mites dominated on the plots covered with Hierochloë repens: Tectocepheus velatus - (1), (2) and (6); Scheloribates laevigatus - (3), Oppiella nova - (4), Eupelops occultus - (5), Liebstadia similis - (7).
PL
Celem pracy było określenie przydatności wybranych organów Robinia pseudoacacia do bioindykacji. Oznaczono zawartości wybranych pierwiastków w liściach, pędach, nasionach i łupinach nasiennych R. pseudoacacia z terenów trzech miast województwa śląskiego: Katowic, Chorzowa i Siemianowic Śląskich. W każdym z miast wytypowano po pięć stanowisk pobrania próbek. Stanowiska podzielono na 3 grupy w zależności od ich otoczenia: różniły się one pod względem poziomu zanieczyszczenia (ruch uliczny, zakłady przemysłowe). Stwierdzono statystycznie istotne różnice w poziomie zanieczyszczeń pomiędzy wyróżnionymi grupami stanowisk. Na podstawie analizy skupień oraz PCA dla poszczególnych organów wyróżniono grupy stanowisk ze wzglądu na koncentrację badanych pierwiastków w tkankach R. pseudoacacia. Przeprowadzone badania pozwalają stwierdzić, że najlepiej nadającym się do celów bioindykacyjnych organem R. pseudoacacia są liście.
EN
The aim of this study was to determine the suitability of chosen organs of Robinia pseudoacacia as bioindicators. Content of selected elements in leaves, shoots, seeds, pods and bark of R. pseudoacacia from the area of three cities of Silesia province: Katowice, Chorzow and Siemianowice Slaskie was estimated. In each of the cities five sampling points were selected. Localizations were grouped in three categories differing in terms of the level of pollution (traffic, industrial plants). Statistically significant differences between pollution level of segregated groups in investigated localizations were found. On the basis of PCA and cluster analysis for individual organs sampling points were divided into groups depending on concentration of investigated elements in tissues of R. pseudoacacia. Conducted studies suggest that the organ best suitable for bioindication are leaves of R. pseudoacacia.
EN
The paper presents the results of research to assess the degree of transformation of forest ecosystems in the area of influence of the Zhytomyr landfill (Ukraine) - one of the typical Ukrainian landfills, which is operated with minimal implementation of environmental protection measures. The study was conducted to identify the relationship between the condition of plantations and the functioning of municipal solid waste landfills. In the study of the estimation of the transformation degree of forest ecosystems in the area of influence of the municipal solid waste landfill in Zhytomyr, it was established that in the studied areas there was a decrease in bonitet by I-II classes, completeness by 0.1-0.2 units, growth by 13-35% in comparison with background values. On the basis of the research of the condition of pine plantations by categories of vital activity of trees the integral indicator was calculated – the index of the condition of forest stands, which characterizes the degree of their damage in the event of aerial pollution of soil and groundwater. It was determined that the maximum values of the index of forest stands condition were observed in the area closest to the landfill (2.88 units), i.e, these are severely weakened stands, the minimum in the control (1.28). Based on the results of the studies, it was established that Scots pine is a convenient and acceptable bioindicator for assessing the state of the environment in the area of influence of municipal landfills provided that its distribution is uniform on the territory and it is available for research. The sanitary condition of pine plantations deteriorates with the approach to the source of pollution. According to the established indices of the condition of pine plantations, a regression analysis was performed and a mathematical dependence was determined, which most accurately describes the change of these indicators with the distance from the landfill boundary. The approach used for bioindication studies of conifers can be recommended for implementation in practice as a method for assessing the transformation of the environment in the area of influence of municipal solid waste landfills.
EN
The article presents the results of monitoring the soil mycobiota of sulfur quarries in LvivRegion. The dynamics of soil micromycete complexes as a result of their adaptation to the conditions of technozems, embryozems and zonal soils of sulfur quarries was studied. The increase in the number of colony-forming units (CFU) in the studied soils of the Yavoriv and Podorozhnie sulfur quarries over the years is specified, which indicates the active processes of decomposition of plant remains, the formation of stable processes of decomposition of cellulose and other complex compounds in the detrital block of the ecosystem. The change in the frequency of banding of selected species of micromycetes over the years was studied, depending on the content of SO4-2 in the soil solution. The functional structure of soil micromycete communities after sulfur extraction was established. A decrease in the frequency of occurrence of pathogens and toxin-producing agents and an increase in the frequency of occurrence of species that actively participate in the transformation of organic remains, and also act as plant endophytes and participants in mycorrhizal symbiosis were recorded. The species composition of micromycetes that grew in the presence of SO4-2 content in the soil solution, which exceeded the background level from 3.6% to 50%, was determined, the species-bioindicators of soil pollution with sulfur compounds were defined - Fusarium oxysporum, Paecilomyces lilacinum, P. waksmanii, P. nigricans, P. funiculosum, Trichoderma viride, Cladosporium cladosporioides, C. herbarum, Aureobasidium pullulans, Humicola grisea, Ulocladium consortiale, Alternaria alternate, Mortierella ramanniana var. angulispora. During 10 years of research a decrease in the content of SO4-2 in soils was noted, which is associated with an increase in the biodiversity of soil micromycetes and an enrichment of the phytocenotic cover.
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