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1 Opto-Electronics Review

1 2003

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Low cost lasers challenge ultrafast systems in two-photon excitation applications

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Tirri M. , Vaarno J. , Soini J. , Hanninen P.

Opto-Electronics Review

2003

tom Vol. 11, No. 1

39-44

Although excellent research tools, the ultrafast laser systems are poorly suited for routine laboratory instrumentation, simply due to their high price. Our research group has shown tat application of two-photon excitation need not to be limited to those laboratories that can afford the price and maintenance of ultrafast laser systems. With certain compromises and well-designed experiments, low-cost lasers can be used in utilizing the positive properties of two-photon excitation. The purpose of this publication is to show examples of bioanalytical applications that are possible using low-cost lasers. A bioaffinity assay using microparticles as bioactive carriers and assay for characterization of cell surface antigens are presented. By direct comparison of the example assays in two instrumental set-ups, a quantity is given to the 'compromises' that are made by replacing an ultrafast system with low cost equipment.