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Surface Energy of Solids: Selection of Effective Substrates for Bioadhesion in Aqueous Media

100%

Boniewicz-Szmyt K. , Pogorzelski S.

Scientific Journal of Gdynia Maritime University

2019

tom nr 112

7--22

Surface wettability of model solids of different hydrophobicity (from hydrophilic to hydrophobic) in contact with an aqueous medium was determined by measuring the dynamic contact angles (CA) using common techniques: sessile drop, inclined plate and captive bubble. The surface wettability energetics parameters: contact angle hysteresis (CAH), 2D adhesive layer pressure, surface free energy (SFE) and work values of cohesion, adhesion and spreading were determined using the formalism proposed by Chibowski [2003]. CA values depended on the technique used and experimental conditions (flow numbers, spatial heterogeneity and roughness of the sample). The most effective substrates for testing bioadhesion on solids submerged in aqueous media were hydrophilic surfaces (SFE ~ 40–58 mJ m-2; CAH ~ 16–20 mN m-1).

Involvement of interfacial instability in morphological changes of some membrane-bound bodies

86%

Coakley W. T.

Cellular and Molecular Biology Letters

1996

tom 01

nr 3

Systems consisting of single membranes or of interacting membranes with an intervening aqueous layer can be modelled as interfacial regions. Treatments of the stability of thin fluid films against bending and squeezing waves give guidance on the behaviour of such regions. Evidence of bending wave instability on erythrocyte membranes when the spectrin component of the membrane skeleton is denatured is presented. There are two alternative outcomes of such instability. In one case a regular surface wave grows on the rim of the erythrocyte at rates of the order of 3 s-1 and vesicles pinch from the wave crests. In the second outcome the cell rounds up and the membrane partially internalises a discoid volume of suspending phase. The rim of that volume becomes unstable and sends vesicles into the cell. The outcome (internalisation or externalisation of membrane) can be controlled experimentally by altering the surface charge or the transmembrane potential or by exposure to amphiphilic drugs. When two interacting membranes of normal erythrocytes at room temperature adhere in the presence of adhesion-inducing polymers or of lectins two distinct adhesion outcomes are also possible. In one case parallel membranes form a continuous seam. In the second outcome localised regions of close contact formed at regular intervals along the membrane surface are separated by regions of larger normal separation. The characteristic lateral spacing of contact regions changes with different modifiers of the membrane interaction profile in a manner consistent with interfacial instability theory.

Rheological and textural properties of hydrogels, containing sulfur as a model drug, made using different polymers types

58%

Wróblewska M. , Słyż J. , Winnicka K.

Polimery

2019

tom T. 64, nr 3

208--215

The preparation technology and the optimal composition of hydrogels with sulfur prepared using various types of polymers – hydroxyethyl cellulose (HEC), Carbopol 980, and sodium alginate – have been developed. Designed hydrogels were evaluated microscopically, for pH, viscosity and mechanical parameters. In addition, ex vivo bioadhesive properties of obtained hydrogels with hairless mice skin model as adhesive layer were estimated. Hydrogels with sulfur based on sodium alginate possessed the most favorable application properties and were stable at different temperature and humidity conditions during 90 days of storage.

Opracowano technologię otrzymywania i optymalny skład hydrożeli z siarką sporządzonych z wykorzystaniem różnych rodzajów polimerów: hydroksyetylocelulozy (HEC), Carbopolu 980 oraz alginianu sodu. Przygotowane hydrożele oceniano mikroskopowo, poprzez pomiary pH i lepkości oraz na podstawie analizy ich właściwości mechanicznych. Ponadto przeprowadzono badanie bioadhezji ex vivo z wykorzystaniem skóry bezwłosych myszy jako modelu warstwy adhezyjnej. Hydrożele z siarką na bazie alginianu sodu charakteryzowały się najlepszymi właściwościami aplikacyjnymi i były trwałe podczas 90 dni przechowywania w różnych warunkach temperatury i wilgotności.