Wyniki wyszukiwania - Biblioteka Nauki

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Humoralna odpowiedz na wybrane antygeny paleczek Yersinia enterocolitica i Yersinia pseudotuberculosis w przebiegu jersiniozy u ludzi. II. Wystepowanie przeciwcial dla wydzielniczych bialek Yop wykrytych odczynem western-immunoblotting

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Rastawicki W.

Medycyna Doświadczalna i Mikrobiologia

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2006

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tom 58

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nr 4

321-328

PL

Oceniono odczynem western-immunoblotting częstość występowania przeciwciał dla poszczególnych wydzielniczych białek Yop w próbkach surowicy osób z różnymi postaciami jersiniozy. Najczęściej w próbkach surowicy osób z objawami bólów brzucha, zapalenia stawów i rumienią guzowatego wykrywano przeciwciała dla białka YopD o masie 36 kDa. Przeciwciała klasy IgG dla białek YopD, YopM i YopH występowały w podobnym odsetku u osób z objawami bólów brzucha i zapaleniem stawów, przeciwciała klasy IgA dla tych białek dominowały u osób z zapaleniem stawów natomiast przeciwciała klasy IgM u osób z objawami bólami brzucha.

EN

The results obtained with the use of the western-blotting showed that antibodies for released proteins YopD (33-36 kDa) were the most frequently detected antibodies in serum samples from patients suspected for yersiniosis. Reactions between serum samples studied and the YopD proteinwere very intense, suggesting that protein is the strongest immunogen among the utilised, released proteins Yop of Yersinia. Antibodies IgM were more often diagnosed in patients with abdominal pain in the contrary to antibodies IgA which were characteristic to patients with reactive arthritis. Detailed analysis of the results of western-blotting on serum samples obtained several times from individuals with yersinosis during the course of infection in this investigation have showed also that antibodies of the IgA class hold longer in serum of individuals with arthritis compared with individuals with yersinosis not complicated by arthritis. In joint-fluid samples obtained from patients with arthritis antibodies for particular released proteins Yop were detected in the same class of immunoglobulins like in serum samples obtained from those individuals.

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Bialka Yop rodzaju Yersinia - mechanizm sekrecji i translokacji

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Gorka A. , Brzostek K. , Hrebenda J.

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tom 38

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nr 2

141-162

3

Humoralna odpowiedz na wybrane antygeny paleczek Yersinia enterocolitica i Yersinia pseudotuberculosis w przebiegu jersiniozy u ludzi. I. Wystepowanie i poziom przeciwcial dla somatycznych antygenow paleczek Yersinia oraz wydzielniczych bialek Yop wykrytych odczynem ELISA

100%

Rastawicki W.

Medycyna Doświadczalna i Mikrobiologia

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2006

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tom 58

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nr 4

303-319

PL

Analiza wyników badań próbek surowicy uzyskanych od osób chorych podejrzanych o jersiniozę wykazała, że humoralna odpowiedź zależna jest od objawów klinicznych, wieku badanych osób, płci i okresu choroby. Najczęściej, bez względu na dominujące objawy kliniczne, wykrywano przeciwciała dla antygenu 03 Y. enterocolitica w badanych klasach immunoglobulin A, G i M, najrzadziej zaś dla antygenu 08 pałeczek tego gatunku.

EN

The antibodies against the somatic antigens of Y. enterocolitica 03, 08, 09, 05,27,Y. pseudotuberculosis I, and released proteins Yop were detected using the ELISA in 1634 serum samples and 84 synovial fluids collected from 1290 persons suspected for yersiniosis, as well as 200 serum samples from healthy individuals (blood donors). The presence of antibody in diagnostically significant titres for somatic antigens of Yersinia were detected by ELISA in 20.5% and 50.6%, antibodies for released proteins Yop in 11.5% and 28.4% respectively of blood donors and patients suspected for yersiniosis. The antibody against the 03 antigen of Y. enterocolitica was the most frequently detected antibody while the most infrequent was the antibody for the antigen from the 08 serologic group. The results of the study showed that the humoral response picture to Yersinia antigens in the course of yersiniosis in humans is dependent on the age and sex of the patient, duration of the infection, and clinical manifestations. Most frequently the elevated antibody levels were detected among patients with erythema nodosum and patients with gastrointestinal symptoms. The frequency of occurrence of antibodies for most antigens of Yersinia, together with age increased reaching its peak, on the average, among individuals aged 21-40 years. Analysis of individual cases showed that by the end of the first week of infection, elevated levels of antibodies for somatic antigens of Yersinia are evident. On the other hand, antibodies for released proteins Yop as a matter of rule appear in the second week from the onset of clinical symptoms. Within this early phase of infection immunoglobulins of the A and M classes dominate reaching their highest level in the second to third week of the infection. In majority of the individuals studied antibodies of the IgG class reached their highest level much later in relation to those of the IgA and IgM classes. Significant differences were found in IgA antibody detection among individuals with clinical manifestations of stomachaches and arthritis. Nevertheless, among individuals with clinical symptoms of stomachaches, these immunoglobulins as a matter of principle disappear with a period of 2-3 months from the onset of clinical symptoms. In individuals with arthritis however the aforementioned immunoglobulins maintained at considerable levels even after a year. In joint-fluid samples obtained from patients with arthritis antibodies for Yersinia antigens were detected in similar levels just as obtained simultaneously serum from those individuals.