Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

2 Medycyna Weterynaryjna

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: bialko Ki-67

Sortuj według:

Ogranicz wyniki do:

Porownanie ekspresji receptorow estrogenowych i progesteronowych w gruczolakorakach gruczolu sutkowego u suk z aktywnoscia mitotyczna komorek nowotworowych

100%

Nowak M. , Madej J. A. , Dziegiel P.

nr 10

1211-1215

The study aimed at monitoring the expression of oestrogen (ER) and progesterone (PGR) receptors in mammary gland adenocarcinomas in bitches as compared to the mitotic activity of the neoplastic cells. Material for the study involved samples of mammary tumors obtained during surgery from 48 bitches of various races, aged 7 to 15 years. The obtained preparations were microphotographed and the patterns were subjected to computer-assisted image analysis in a system consisting of a computer linked to an Axiophot light microscope (Carl Zeiss). The expression of ER, PgR and of Ki-67 was evaluated using a semi-quantitative scale which involved evaluating the proportion of positive cells (0-5% no reaction [], 6-25%: weak reaction [+], 26-50%: moderate reaction [++], above 50%: intense reaction [+++]). The evident deficit of ER observed in our studies might indicate an oestrogen independency of the adenocarcinomas in bitches. This, however, does not preclude involvement of the receptors at earlier stages of carcinogenesis. The obtained strong correlation between expressions of Ki-67 and that of PgR (r=0.67), in view of the high prognostic value of Ki-67 expression, confirmed in numerous studies, allow the conclusion that presence of progesterone receptors in the cells of mammary gland adenocarcinoma in bitches is linked to the augmented proliferative potential of the tumors, similarly as it has been demonstrated in mammary glands in females.

Ekspresja białka Ki-67, PCNA i indeksu apoptotycznego w przebiegu nieswoistego zapalenia jelit u psów z oceną ich ich wartości diagnostycznej

80%

Rychlik A. , Nowicki M. , Szweda M. , Kaczmar E.

nr 09

The aim of this study was to determine the expression of Ki-67 and PCNA proliferative antigens and the apoptotic index in dogs with various degrees of severity of inflammatory bowel disease (IBD), and to evaluate the applicability of these markers in diagnosing the disease. The study was performed on 28 dogs of different breeds, both sexes, with body weights of 10 to 20 kg, aged 2 to 10 years. The animals diagnosed with IBD were divided into four groups of 7 dogs each, including three groups characterized by various degrees of severity of IBD and a control group. The expression of Ki-67 and PCNA antigens was determined immunohistochemically, and the apoptotic index was expressed as the number of TUNEL-positive lamina propria cells in the duodenum, jejunum and the colon. Regardless of its form, IBD affected the expression of the analyzed proliferation markers and apoptosis in duodenal, jejunal and colonic mucosa, but most of the analyzed parameters were not correlated with the severity of the disease. The severity of IBD was correlated only with the expression of Ki-67 in duodenal villi. The results of the study point to the limited applicability PCNA and the apoptotic index in differentiating and evaluating the progression of IBD in dogs. The estimation of Ki-67 expression may be useful in the evaluation of the severity of IBD in dogs.