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EN
The aim of the present study was to determine the concentration of C-reactive protein (CRP) and serum amyloid A (SAA) in bitches suffering from spontaneously occurring mammary gland tumors. The experiment involved 30 bitches with malignant gland tumors removed surgically (carcinoma complex - 40%, carcinoma simple - 26.7%, sarcoma - 23.3%, carcinosarcoma - 10%) and 10 clinically healthy bitches. Measurements of the CRP and SAA serum concentration were performed using a commercial ELISA test. The concentration of CRP as well as SAA were significantly higher in bitches with sarcomas or carcinosarcomas compared with the animals with carcinomas (complex or simple) and the control group. Histopathological examination showed extensive areas of necrosis and inflammatory reactions in most sarcomas and carcinosarcomas. Most likely these were the main causes of the CRP and SAA increase in the serum of bitches with these type of tumors.
EN
The aim of the present study was to determine the cooperative interactions among some non-specific humoral factors participating in the immune response of healthy suckling piglets and piglets with diarrhea caused by E.coli at different ages. A total of 168 hybrid piglets (aged 12 to 18 days and 19 to 25 days), clinically healthy and suffering from diarrhea, were examined. The serum levels of lysozyme, ceruloplasmin (CP) and C-reactive protein (CRP) were determined in randomly selected sibs (clinically healthy piglets and piglets with diarrhea). Feces samples were taken from piglets with diarrhea to identify E. coli strains. A positive correlation between lysozyme activity and the serum concentration of CP was observed in healthy animals. This shows that over the experimental period the values of both these indices increased along parallel lines at a relatively stable CRP level. Elevated serum levels of lysozyme, CP and CRP in piglets with diarrhea caused by E. coli, as compared with healthy piglets, reflected the participation of these non-specific humoral factors in the immune response against E. coli. The increase in lysozyme activity, resulting from the immune response, was parallel to the increase in CP concentration (a positive correlation), but not parallel to the increase in CRP concentration (a negative correlation). These changes were found to be age-related. In younger piglets, the lysozyme activity was significantly correlated with CRP level but in older ones with level of CP.
EN
Searching for new more accurate biomarkers in order to evaluate the health of bitches with reproductive disorders and disturbances has been a subject of inquiry for many researchers. Biomarkers are measurable indicators, the concentrations of which are dependent on the degree and type of stimulus. Serum C-reactive protein (CRP) has been proven to be a valuable biomarker for health status evaluation of bitches suffering from reproductive disorders, such as pyometra, as well as for treatment monitoring. However, no data are available related to the CRP profile in bitches with ovarian cysts for a better evaluation of their condition and prognosis. Thus, the aim of this study was to determine the possible usefulnessof CRP determination in the serum of bitches with ovarian cysts. Our results suggest that CRP concentrations could be useful indicators of the presence of ovarian cysts, although studies with more animals are needed.
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