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Laminin- and basement membranepolycaprolactone blend nanofibers as a scaffold for regenerative medicine

100%

Neal R. A. , Lenz S. M. , Wang T. , Abebayehu D. , Brooks B. P. C. , Ogle R. C. , Botchwey E. A.

Nanomaterials and the Environment

2014

tom 2

nr 1

Mimicking one or more components of the basement membrane (BM) holds great promise for overcoming insufficiencies in tissue engineering therapies. We have electrospun laminin nanofibers (NFs) isolated from the murine Engelbreth-Holm Swarm (EHS) tumor and evaluated them as a scaffold for embryonic stem cell culture. Seeded human embryonic stem cells were found to better maintain their undifferentiated, colony environment when cultured on laminin NFs compared to laminin mats, with 75% remaining undifferentiated on NFs. Mouse embryonic stem cells cultured on 10% laminin-polycaprolactone (PCL) NFs maintained their colony formation for twice as long without passage compared to those on PCL or gelatin substrates. In addition, we have established a protocol for electrospinning reconstituted basement membrane aligned (RBM)-PCL NFs within 10° of angular deviation. Neuron-like PC12 cells show significantly greater attachment (p < 0.001) and percentage of neuriteextending cells in vitro on 10% RBM-PCL NFs when compared to 1% and 0% RBM-PCL NFs (p < 0.015 and p < 0.001, respectively). Together, these results implicate laminin- and RBM-PCL scaffolds as a promising biomimetic substrate for regenerative medicine applications.

Deconstructing B cell tolerance to basement membranes

86%

Foster M. H. , Zhang Y. , Clark A. G.

Archivum Immunologiae et Therapiae Experimentalis

2006

tom 54

nr 4

Histochemical changes in the testes of lead induced experimental rats

72%

Chowdhury A. R. , Rao R. V. , Gautam A. K.

Folia Histochemica et Cytobiologica

1986

tom 24

nr 3

Concentrations and activities of metalloproteinases 2 and 9 and their inhibitors (TIMPS) in chronic pancreatitis and pancreatic adenocarcinoma

58%

Lekstan A. , Lampe P. , Lewin-Kowalik J. , Olakowski M. , Jablonska B. , Labuzek K. , Jedrzejowska-Szypulka H. , Olakowska E. , Gorka D. , Filip I. , Dranka-Bojarowska D.

Journal of Physiology and Pharmacology

2012

tom 63

nr 6

Changes in the midgut of larvae of the khapra beetle, Trogoderma granarium Everts, induced by gamma radiation

58%

Szczepanik M. , Ignatowicz S.

Bulletin of the Polish Academy of Sciences. Biological Sciences

1995

tom 43

nr 3-4

Cysteine peptidases and their inhibitors in breast and genital cancer

51%

Agrawal A. K. , Ekonjo G. B. , Teterycz E. , Zysko D. , Grzebieniak Z. , Milan M. , Marek G. , Siewinski M.

Folia Histochemica et Cytobiologica

2010

tom 48

nr 3