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EN
MicroRNAs are 19- to 24-nt-long single-stranded RNAs that are crucial regulators of gene expression which control plant development and response to environmental cues. We have analyzed microtranscriptomes of five barley developmental stages. Generally, during the barley development, miR168-3p and miR1432-5p levels increase while the 5'U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5'U-miR156-5p [20 nt] and 5'UU-miR156-5p [21 nt]), which were expressed differently during barley development. The 5' U-miR156-5p level decreased in 3-week-, 6-week-, and 68-day-old barley, when compared to the 1-week-old plants. Meanwhile, the 5' UU-miR156-5p level increased significantly in the 68-day-old barley plants. Moreover, only the 5' U-miR156 isomiR recognizes and guides unique transcription factor mRNAs from the Squamosa Promoter Binding Protein-Like (SPL) family. We identified many non-canonical microRNAs with changed expression levels during the barley development. Here, we present the profiles of microRNA expression characteristics for particular barley developmental stages. These analyses are accompanied by the experimental degradome analysis of miRNA targets.
EN
Beer consumption constitutes a significant portion of alcoholic beverage consumption in Poland. The growing interest in this product has prompted the exploration of solutions that not only affect production technology and costs but also influence the sensory and physicochemical attributes of the beverage. Incorporating unmalted raw materials is one such solution. This research aims to conduct a comparative analysis of haze formation in malted beers and beers with unmalted barley additions, considering diverse storage conditions and production scales. The results revealed that light has a more significant effect on turbidity formation than temperature. In the variants in which the impact of light on haze formation was investigated, the values of the average number of total particles were in the range of 130 (unsweetened laboratory under ultraviolet) to 1025 (unsweetened commercial under ultraviolet). The effect of temperature on haze formation was significantly less. In most cases, the average number of total particles was no greater than 200, with the highest result obtained being 300 (commercial malted under forced aging). Based on the study, it was concluded that, regardless of the scale of production, ultraviolet radiation causes significant haze formation in beer.
EN
The fundamental aim of this study was to investigate the growth responses of selected Jordanian cultivated barley (Hordeum vulgare L.) genotypes to the salinity stress. Twenty-six landraces and two recent cultivars were subjected to four levels of salinity (0, 50, 100 and 200 mM NaCl). The salt stress was found to influence the majority of germination ability such as germination % which ranged from about 80% to 100% (One-way ANOVA; p ≤ 0.05). Moreover, germination was statistically affected in correspondence to exposure time to salinity and in relation to genotypic composition of studied barley (two-row vs. six-row accessions) (Two-Way ANOVA; p ≤ 0.05). Early seedling growth traits were also found to decline with increasing salinity stress. Moreover, according to the growth parameters genotypes, M’ 1595, M’ 1593, Ir 1558, Ir 1631, Ir 1639, Mf 1545, and Mf 1548 were found to have better performance than others. On the other hand, the genotypes M’ 1593, M’ 1594, M’ 1595, Ir 1558, Ra 1552, Ra 1611, Mf 1616, Mf 1617, and Ma 1592 were most affected genotypes by salinity. The results of this study lead to the conclusion that the response to the salinity stress is complex, yet the comprehensive results found in this study provide a foundation for deeper exploration of diversity as well as the gene–trait relationships and their utilization in future barley improvement.
EN
In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations. Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation. The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants). Plants obtained via embryo callus culture showed good vegetative vigour and well-developed root system. Spike morphology of all plants resembled that of rye. Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The heteromorphic bivalent-like configurations occurred in five plants in 0.01-0.02 per cell. The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with a chiasma frequency of 9.36-17.75 per cell. All plants, with 14 and 28 chromosomes, were completely sterile both in backcrosses and when selfed.
EN
The inheritance of resistance to loose smut (Ustilago nuda) in seven cultivars of spring barley has been examined. The performed studies showed that, resistance to two different groups of U. nuda races in respect of their virulence is determined by a single allele pair in the cvs. Anoidium and Inerme 2-r and by two allele pairs in the cvs. CI 13 662, Dorsett, Jet and OAC 21. In the cv. Abyssinian, resistance to a group of races 2 is determined by a single allele pair, whereas that to a group of races 4 - by two allele pairs. In all studied cultivars (except Anoidium) the resistance dominates over sensitivity. Resistance to the both studied groups of U. nuda races is determined by similar genes in the cvs. Dorsett and CI 13 662, as well as in Dorsett and OAC21, and additionally to a group of races 4 in the cvs. Abyssinian and OAC 21. No similarity was found between resistance genes in the case of two allele pairs in the cvs. Jet, Abyssinian and CI 13 662 (group of races 4) as well as in Jet, Dorsett and OAC 21 (in both groups of races), and in the case of a single allele pair in the cvs. Inerme-2-rowed and Abyssinian (group races 2).
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EN
The usefulness of mutagenic treatment to enlarge isozymic variability of barley and the use of induced mutants for genetic analysis were evaluated. N-methyl-N-nitroso urea, sodium azide and gamma rays were employed as mutagenic agents. Electrophoretic assays of 3848 M₂ seedlings obtained by chemical mutagenic treatment of the spring barley cultivars Dema, Aramir, Bielik and 3100 M₂ seedlings obtained by physical mutagenic treatment of the cv. Dema revealed 70 isozymic mutants, which represent 30 separate mutants in 25 M₁ plants. Most of mutations (27) were induced by chemical mutagen at polymorphic esterase loci. The occurrence of induced mutants at monomorphic loci, Got2 and Lap2, made it possible to perform genetic analysis of those loci in barley including mapping respective genes within chromosomes.
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