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The cytotoxic effect of diphtheria toxin on the actin cytoskeleton

100%

Varol B. , Bektas M. , Nurten R. , Bermek E.

Cellular and Molecular Biology Letters

2012

tom 17

nr 1

Diphtheria toxin (DT) and its N-terminal fragment A (FA) catalyse the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide (NAD) into a covalent linkage with eukaryotic elongation factor 2 (eEF2). DT-induced cytotoxicity is versatile, and it includes DNA cleavage and the depolymerisation of actin filaments. The inhibition of the ADP-ribosyltransferase (ADPrT) activity of FA did not affect the deoxyribonuclease activity of FA or its interaction with actin. The toxin entry rate into cells (HUVEC) was determined by measuring the ADP-ribosyltransferase activity. DT uptake was nearly 80% after 30 min. The efficiency was determined as Km = 2.2 nM; Vmax = 0.25 pmol.min−1. The nuclease activity was tested with hyperchromicity experiments, and it was concluded that G-actin has an inhibitory effect on DT nuclease activity. In thepresence of DT and mutant of diphtheria toxin (CRM197), F-actin depolymerisation was determined with gel filtration, WB and fluorescence techniques. In the presence of DT and CRM197, 60–65% F-actin depolymerisation was observed. An in vitro FA-actin interaction and F-actin depolymerisation were reported in our previous paper. The present study thus confirms the depolymerisation of actin cytoskeleton in vivo.

Divalent cations and protons inhibit Triton X-100 induced ion channels

100%

Rostovtseva T. K.

Biophysics of Membrane Transport

1994

tom 12

nr 2

Effect of different lymphocyte-bacteria ratios and bacterial toxins on apoptosis of bovine mammary gland lymphocytes

84%

Slama P. , Sladek Z. , Rysanek D.

Proceedings of ECOpole

2011

tom Vol. 5, No. 1

109--113

Staphylococcus aureus and Streptococcus uberis delay apoptosis of bovine mammary gland lymphocytes following intramammary infusion and in in vitro studies with lymphocyte-bacteria ratio 1:1. In this study, we investigated the effect of different lymphocyte-bacteria ratios on apoptosis of bovine mammary gland lymphocytes in vitro. We found out that lymphocyte-bacteria (S. aureus or S. uberis) ratios 1:10, 1:50 and 1:100 have different effect on apoptosis of lymphocytes than ratio 1:1. Lymphocyte apoptosis was induced 6 hours following incubation with S. aureus or S. uberis with mentioned ratios (1:10, 1:50 and 1:100). In our previous preliminary experiments focused on exploration of chemical components of bacteria on apoptosis of lymphocytes, we established the effect of muramyl dipeptide and lipopolysaccharide on lymphocyte apoptosis only in vitro. Therefore, in the second part of the present study we focused our experiments on investigation of the effect of Gram-negative bacterial toxin lipopolysaccharide on apoptosis of bovine mammary gland lymphocytes in vivo. The results of these experiments suggest that lipopolysaccharide induces apoptosis of lymphocytes following intramammary application. These data need next exploration to reveal detail effects of bacteria or bacterial toxins on lymphocyte programmed cell death in connection with inflammatory process.

Expansion of the Golgi apparatus in rat cerebral cortex following intracerebroventricular injections of streptozotocin

84%

Grieb P. , Kryczka T. , Fiedorowicz M. , Frontczak-Baniewicz M. , Walski M.

Acta Neurobiologiae Experimentalis

2004

tom 64

nr 4

Review of the phenotypic methods applied to identification of Citrobacter freundii

67%

Pobucewicz A. , Czernomysy-Furowicz D. , Fijalkowski K. , Karakulska J. , Ferlas M.

Advances in Agricultural Sciences

2010

tom 13

nr 1

Emerging food pathogens and bacterial toxins

67%

Bielecki J.

Acta Microbiologica Polonica

2003

tom 52

nr Suppl.

Nowe możliwości zapobiegania chorobie obrzękowej świń

51%

Pejsak Z. , Nalbert T.

2014

tom 09

nr 1

Wplyw kultur starterowych na zmiany poziomu wybranych ksenobiotykow podczas utrwalania surowcow pochodzenia zwierzecego

34%

Steinka I. , Przybylowski P.

Żywność Technologia Jakość

1997

tom 04

nr 4

5-15